Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,373,180	T→C	7.7%	W97R (TGG→CGG)	arnF →	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnF subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,180	0	T	C	7.7%	168.3 / 4.7	65	W97R (TGG→CGG)	arnF	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnF subunit
Reads supporting (aligned to +/- strand):  ref base T (24/36);  new base C (3/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

CTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCT  >  NC_000913/2373039‑2373322                                                                                                                                              |                                                                                                                                               cTGTTATTCGGGCTGCTGGGTTATCTGCTTTGCTTTTTTTTTTGGTATAAAACGCTTCATGAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTg                                                                                                                                               <  2:462237/143‑1 (MQ=255) cTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATATAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCGTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTg                                                                                                                                               <  2:210328/143‑1 (MQ=255) cTGTTACTCGGGCCGCTGGGTTATCTCCTTTCCGTTTTTTGTTCGTATAAATCTCTTCATAATCTTGCTCTAAGCAAAGCCTATGCCTTGCCCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTg                                                                                                                                               <  1:291450/143‑1 (MQ=255)   gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTCTCAAACGCTTCATAAACTTGCGCTAATCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGAATTGCTTCATTGGTACTTCCCGGCTggg                                                                                                                                             >  2:471282/1‑143 (MQ=255)             ctgctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGGTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgtaccatt                                                                                                                                   <  1:1012245/143‑8 (MQ=255)                 tgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCg                                                                                                                               <  1:178479/143‑1 (MQ=255)                 tgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCg                                                                                                                               >  2:220336/1‑143 (MQ=255)                  ggtttATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAATCTTGCGCTATGCAACGCCTATGCCGTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGc                                                                                                                              <  2:309924/140‑1 (MQ=255)                   ggTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCt                                                                                                                             <  2:379893/143‑1 (MQ=255)                     ttATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAATGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTACCGTCTGGGAAGGTACTTTTTCGCTGa                                                                                                                           >  2:996225/1‑143 (MQ=255)                     ttATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGAAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGATGGTATGGATTGCTTCCATCGTACTTCCCGGCTGGGAAGGGACTTTTTCGCGGa                                                                                                                           >  1:636878/1‑143 (MQ=255)                      tATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaa                                                                                                                          <  1:579911/143‑1 (MQ=255)                       aTCTTCTTTCCGTCTCTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATTAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                         <  1:21033/143‑1 (MQ=255)                       aTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                         <  1:547934/143‑1 (MQ=255)                       aTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                         <  1:824194/143‑1 (MQ=255)                       aTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCAGGCTGGGGAGGGGCTTTGTCGCTGaaa                                                                                                                         >  1:227401/1‑143 (MQ=255)                        tCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCTGCTGGGAAGGGACTTTTTCGCTGAAAg                                                                                                                        <  1:294392/143‑1 (MQ=255)                        tCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAg                                                                                                                        <  1:50194/143‑1 (MQ=255)                        tCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTCGGAAGGGACTTTTTCGCTGAAAg                                                                                                                        >  1:167733/1‑143 (MQ=255)                             tttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGCGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGCCTTTTTCGCTGAACGCccta                                                                                                                   >  1:225192/1‑143 (MQ=255)                              ttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCactac                                                                                                                  >  2:586772/1‑143 (MQ=255)                               tCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCCATGGTACTATTTGGTTGTCAAGGGACTTTTTCGCTGAAAGCactact                                                                                                                 <  1:1002194/143‑1 (MQ=255)                                 cGTTTTTGCTTGCTATCAAACGCTTCCTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTgg                                                                                                               <  1:113985/143‑1 (MQ=255)                                 cGTTGTTTGTTGGTATAGACCGCGTCATACCCCTGCGCTAAGCAACGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTgg                                                                                                               <  1:313831/143‑1 (MQ=255)                                     ttttG‑TGGTATTCAACGCCTCATAAACTTGCGCTCAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTa                                                                                                          <  2:647324/143‑1 (MQ=255)                                       tcgttgGTATAGAACGCTGCACACACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGCTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGCCTTTTTCGCTGAAAGCACTACTGGGAGTAg                                                                                                         <  2:572915/141‑1 (MQ=255)                                        tgttgGTATAAAACGCTTCATCAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGc                                                                                                        <  1:637654/143‑1 (MQ=255)                                         gttgGTATAAAGCGCTTCATAGACGTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGcc                                                                                                       <  2:585734/143‑1 (MQ=255)                                          ttgGTATAACACGCTTCATTAGCCTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCGGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCt                                                                                                      <  2:262861/143‑1 (MQ=255)                                                  aaaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCTATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATg                                                                                              >  2:579164/1‑143 (MQ=255)                                                     cGCTTGATAAAGTTGCGCGAGGCAATGCCTATGCCTTGGTCAGTATGAGTGATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTAGTATGAGc                                                                                           <  2:235475/143‑1 (MQ=255)                                                         tCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGt                                                                                       <  1:294297/143‑1 (MQ=255)                                                                  tGCGCTGAGCAAAGCCTATGCCTTGCTCAGTATGAGCTATGTGCTGGTGTGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGACAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGa                                                                              <  2:427847/143‑1 (MQ=255)                                                                     gcTGAGGAAAGCCTATGCCTGGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAttt                                                                           <  2:406357/143‑1 (MQ=255)                                                                         aGCAAACCCTATGCCTTGCTCCGTATGCTGTATGTGCTGGTATTGATTGCTTCAATGGTACTTCCCGGCTGGGAACTGACTTTTTCGCTCAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCt                                                                       <  1:15447/143‑1 (MQ=255)                                                                                 cTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGa                                                               <  1:342741/143‑1 (MQ=255)                                                                                       cTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAGaac                                                         >  1:431090/1‑143 (MQ=255)                                                                                          tcTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCTATGGTACGTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaacaac                                                      <  2:766517/142‑1 (MQ=255)                                                                                          gCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaacaac                                                      <  1:220336/143‑1 (MQ=255)                                                                                              aGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTa                                                  >  2:268003/1‑143 (MQ=255)                                                                                              aGTATGAGTGATGTGCTGGTACGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAACGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTa                                                  <  2:731649/143‑1 (MQ=255)                                                                                                 aTGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTg                                               >  2:579333/1‑143 (MQ=255)                                                                                                      ttATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGCGCGGGTCGATGCTGATTCTCCTGACCACGACCGAACAACGGTACTGAGttc                                          >  2:1043083/1‑142 (MQ=255)                                                                                                         tgtgCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTAATTGGAGTAGCCTGCCATATCGTCTTGTTGATGCTGATTTGCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                       <  2:469262/143‑1 (MQ=255)                                                                                                          gtgCTGGTATGGCTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTccc                                      <  1:100217/143‑1 (MQ=255)                                                                                                          gtgCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGGTTTccc                                      >  2:439906/1‑143 (MQ=255)                                                                                                                gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCAc                                >  2:770044/1‑143 (MQ=255)                                                                                                                     gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTac                           <  1:586772/143‑1 (MQ=255)                                                                                                                           ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGACAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCACTGCCCCTTTACACTccc                     >  2:1029552/1‑143 (MQ=255)                                                                                                                           ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAGAACAACGTTACTGAGTTTTCCCTGCCGCTTTACACTGcc                     >  1:333053/1‑143 (MQ=255)                                                                                                                           ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCCGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                     >  1:717163/1‑143 (MQ=255)                                                                                                                              aaTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCCATACAAAGTTTGGCCTGTATTATGAGCGGCTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGtt                  <  1:718123/143‑1 (MQ=255)                                                                                                                               ctGGTACTGCCCGGCTGGGAAGGGACGTTTTCGCTGAAAGCACTGCTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTc                 <  2:924479/142‑1 (MQ=255)                                                                                                                               aTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTc                 <  1:268003/143‑1 (MQ=255)                                                                                                                                agtcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                >  1:945266/6‑143 (MQ=255)                                                                                                                                  tcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCAGTATTCTGAGCGGGCAGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCa              >  1:663606/4‑143 (MQ=255)                                                                                                                                  gcaCTGCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCa              <  1:471282/141‑1 (MQ=255)                                                                                                                                  gTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGCGTAGCCTGTATTATGAGCGGGTTGATGCTGAGTTTCCTGCCCACGACCAAACAACGTTCCGGAGCTTTCCCTGCCACTCTACACGGCCGTTCCCa              >  2:37545/1‑143 (MQ=255)                                                                                                                                   cccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAttt                                                                           <  1:273428/79‑1 (MQ=255)                                                                                                                                   cccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAttt                                                                           >  2:273428/3‑81 (MQ=255)                                                                                                                                     cTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTc           >  1:518651/1‑143 (MQ=255)                                                                                                                                      ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCa          >  2:96861/1‑143 (MQ=255)                                                                                                                                      ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCACCTTTAGACTGCGGTTCCCAGTCa          >  1:121816/1‑143 (MQ=255)                                                                                                                                         ccGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTAACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCg       >  1:893267/1‑143 (MQ=255)                                                                                                                                          cGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGACAGTGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCACCgg      >  1:149266/1‑143 (MQ=255)                                                                                                                                              tGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCt  >  1:604010/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                               CTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCT  >  NC_000913/2373039‑2373322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑