Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,394,770 T→C 17.7% T93A (ACT→GCT)  nuoL ← NADH:quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,394,7700TC17.7% 75.0 / 5.0 39T93A (ACT→GCT) nuoLNADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (19/11);  new base C (2/6);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATC  >  NC_000913/2394628‑2394896
                                                                                                                                                |                                                                                                                              
tACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGt                                                                                                                                <  2:278236/143‑1 (MQ=255)
   aTCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTGGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGac                                                                                                                             >  1:747746/1‑143 (MQ=255)
   aTCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGac                                                                                                                             >  1:9103/1‑143 (MQ=255)
       gcagcagGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATAAAAGG‑GGAGTCACATGTCAATAAGG‑AAAGCCACACCCGCGaccacc                                                                                                                         >  2:741910/1‑143 (MQ=255)
         agcagGTTGTCGGCAAGCACCAGAACCCCCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCga                                                                                                                       <  1:52293/143‑1 (MQ=255)
               ttGTCGGCAAGCACCAGAACCACCATGCTGGGGATGAACAGGCTGGTGCAAGCGAAGACGCGAGCGTAGCCCTCTTCACCGCGCACATACCGGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGCGACCACCGAGAGCag                                                                                                                 >  2:886470/1‑142 (MQ=255)
               ttGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCAt                                                                                                                 >  1:603547/1‑143 (MQ=255)
                 gTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTGCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCCGTGACCACCcagcgagcgc                                                                                                               >  2:763291/1‑136 (MQ=255)
                     gCAAACTCCAGAACCCCCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGTATATGG‑TTTACTGAACCGGCGACCACCGAGAGCATGGTCAg                                                                                                           <  2:718942/143‑1 (MQ=255)
                       aaGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACAGCAGTGACCACCGAGAGCATGGTCAGCg                                                                                                         >  1:967083/1‑143 (MQ=255)
                              aGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGcc                                                                                                  <  1:729647/143‑1 (MQ=255)
                                     ccaTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑GAACCAACACCAGGGACCACCGGGAGCATGGCGAGCGGCAGACCGACGAGc                                                                                           >  2:334187/1‑143 (MQ=255)
                                     ccaTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCCTGGTCAGCGACGGGCCGTCCAGc                                                                                           >  1:846231/1‑143 (MQ=255)
                                       aTGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCAc                                                                                         <  1:339729/143‑1 (MQ=255)
                                           tGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAgg                                                                                     <  1:828240/143‑1 (MQ=255)
                                                    aCAGGTAGGTGGAAGCGAAGAAGCGGGGCTGGTCATATCCACCGCACATAGCCCAGGGGGCGT‑ACATGTGAATATGGTTTAATGATA‑CGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCg                                                                            <  2:218244/143‑1 (MQ=255)
                                                     caggggggTGTAAGCGAAGAAGCGAGAGTAGCCCGCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGa                                                                           <  1:445403/137‑1 (MQ=255)
                                                     cAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCCGGTCAAACCCGa                                                                           >  1:748504/1‑143 (MQ=255)
                                                     cAGGTGGGCGTGAGCGAAGAAGCGATGGCAGCCCTTTTCACCCCCCATATACCGGCAGGCGT‑GCATGTCAATTTGT‑TTTAACGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGa                                                                           <  2:925311/143‑1 (MQ=255)
                                                      aGGTTGGTGCAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGAt                                                                          <  1:741910/143‑1 (MQ=255)
                                                                        aagCGTGAGTAGCCCTCTTCACCGCGCATCTACCAGGAGGCGT‑ACATGTGATTAAGG‑AATCCCGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGa                                                        <  2:868362/143‑1 (MQ=255)
                                                                           cGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACAt                                                     >  2:99803/1‑143 (MQ=255)
                                                                                 tAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGt                                               >  1:412357/1‑143 (MQ=255)
                                                                                 tAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGt                                               <  1:747876/143‑1 (MQ=255)
                                                                                    cccTCCGCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCca                                            <  1:602836/143‑1 (MQ=255)
                                                                                       tctTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAg                                         >  1:421735/1‑143 (MQ=255)
                                                                                       tctTCACCGCGCATATACCACGAGGAGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCCGCACCAGGTTAACACCGATGTTAAAGTCGCCTCCCGACACCCCCGTCcaccg                                         >  1:98087/1‑141 (MQ=255)
                                                                                            aCCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCggct                                    >  1:141233/1‑143 (MQ=255)
                                                                                               gctcATATACCAGGTGGCGT‑ACATGTGTTTAATG‑AATCCTGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCgactggc                                 <  2:87102/140‑1 (MQ=255)
                                                                                               gcgcATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCggctggc                                 >  1:757573/1‑143 (MQ=255)
                                                                                                cgcATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGCCAGCGCCAGGCCGTCCAGCACCAGGCCAAAACCGATGATAAAGTCCCCTACCGACATCCACCTCCCGACCggcgggct                                >  2:204049/1‑143 (MQ=255)
                                                                                                      gcccgggagtCGT‑ACGTGTGATTAGGG‑TACCCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGGCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGt                          <  1:334187/138‑1 (MQ=255)
                                                                                                       aCCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGACAGCGACAGGCCCTCCAGCACCAGGTTAAAGCCGATGGCAAAGACGCCCACGGACAGACACGCGCACAGCGGCCGGCCGtcaggc                         >  2:203139/1‑138 (MQ=255)
                                                                                                           ggaggCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCt                     <  1:99803/143‑1 (MQ=255)
                                                                                                               gCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGcc                 >  1:199593/1‑143 (MQ=255)
                                                                                                               gCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGACCAGCACCAGGTGAAAACCGGCGCTAAAGTCGCCTACCGACATACACCTCCACAGCGGCTGGCTGTAGGTCTGCCCGcc                 >  2:939420/1‑143 (MQ=255)
                                                                                                                    aCATGGGAATTAGG‑TTTCCCGCTCCGGCGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTa             <  2:234579/143‑1 (MQ=255)
                                                                                                                           aaTAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGACAGCGACAGGCCGTCCAGCACCCGGTTAAAACCGAGGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGGCTGCTCGCCGCTAGCgaagc      >  2:523330/1‑142 (MQ=255)
                                                                                                                            agaaGG‑ACACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCgaagaa     <  1:502535/141‑1 (MQ=255)
                                                                                                                               aGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATc  >  1:724440/1‑143 (MQ=255)
                                                                                                                                                |                                                                                                                              
TACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATATACCAGGAGGCGT‑ACATGTGAATAAGG‑AAACCCACACCAGTGACCACCGAGAGCATGGTCAGCGACAGGCCGTCCAGCACCAGGTTAAAACCGATGTTAAAGTCGCCTACCGACATCCACGTCCACAGCGGCTGGCTGTATGTCTGCTCGCCGTTAGCGAAGAAATC  >  NC_000913/2394628‑2394896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑