Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,432,990	G→C	9.7%	intergenic (‑48/+22)	folC ← / ← accD	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase/acetyl‑CoA carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,990	0	G	C	9.7%	171.5 / 12.0	62	intergenic (‑48/+22)	folC/accD	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase/acetyl‑CoA carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base C (2/4);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

GCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGC  >  NC_000913/2432850‑2433129                                                                                                                                             |                                                                                                                                            gCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTg                                                                                                                                           >  2:820426/1‑143 (MQ=255)    gagaTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCTACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTAAGGTGAGAATAGCACAAGGGCAGAACCAGTGgcc                                                                                                                                        >  2:231150/1‑143 (MQ=255)    gagaTCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATCATCATGGTATCCGCTGATTCGTTACGGTGGGAATAGCAAAAGGGCAGAGCCAGTGgcc                                                                                                                                        >  1:7682/1‑143 (MQ=255)          gATAGTTTCACCGTGCAGGTGTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgccc                                                                                                                                  <  2:4008/143‑1 (MQ=255)           aTAGTTTTCCTGTGCAGGTTTGCCAGATAAGAAGGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgccct                                                                                                                                 <  2:607062/143‑1 (MQ=255)           aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgccct                                                                                                                                 >  2:304249/1‑143 (MQ=255)            tAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtt                                                                                                                                <  1:803074/143‑1 (MQ=255)            tAGGTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtt                                                                                                                                <  2:857974/143‑1 (MQ=255)              gTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttat                                                                                                                              <  1:837976/143‑1 (MQ=255)                tttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                            <  2:592998/143‑1 (MQ=255)                tttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATAATCGTATTCTCGGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                            >  1:241597/1‑143 (MQ=255)                   acggggCAGGTTGTCCAGAGAAGAAAGCCACGGAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTCCGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagtt                                                                                                                         <  1:458679/138‑1 (MQ=255)                       tgCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCTCTGCCCttatcagttatca                                                                                                                     >  2:534673/1‑143 (MQ=255)                       ggCAGGTTTTCGCGCTAAGACAGTCACGAGGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTAGATAATCGTGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatca                                                                                                                     <  2:875056/142‑1 (MQ=255)                             ttttCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCACCGCCCTTATCAGTTATCAGGCCTc                                                                                                               >  2:477435/1‑143 (MQ=255)                                  cAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGCTTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGtt                                                                                                          >  1:893686/1‑143 (MQ=255)                                  cAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGACAGAGCCAGTGGCCCTGCCCTTATGAGTGATCATGCCTCAGGtg                                                                                                          >  1:209948/1‑142 (MQ=255)                                   aGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGGTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCGGCCCTTATCAGTTATCAGGCCTCAGGGTc                                                                                                         >  1:1028961/1‑143 (MQ=255)                                    gATAGGAAAGCCACGAAGCCATAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTcc                                                                                                        <  2:953943/143‑1 (MQ=255)                                        aGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGAt                                                                                                    <  2:959220/143‑1 (MQ=255)                                        aGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGAt                                                                                                    <  1:423530/143‑1 (MQ=255)                                          aaaGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATcc                                                                                                  >  1:171806/1‑143 (MQ=255)                                           aaGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCg                                                                                                 <  1:748727/143‑1 (MQ=255)                                            aGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATACTCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCgg                                                                                                >  2:73313/1‑143 (MQ=255)                                            aGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCgg                                                                                                >  1:843855/1‑143 (MQ=255)                                                   aaGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCggg                                                                                         <  2:716881/143‑1 (MQ=255)                                                     gcccgtggCGACGCGGCTGGAGGAGTGCGTGTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAGGGGGCAGGGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCggggg                                                                                       <  2:1037904/137‑1 (MQ=255)                                                       cAGAGGCGACGCGGCTTGAGGAGTGCTTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTa                                                                                     <  1:334071/143‑1 (MQ=255)                                                       cAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACGGGGGGTa                                                                                     >  1:847094/1‑143 (MQ=255)                                                        agagGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTAc                                                                                    <  1:126956/143‑1 (MQ=255)                                                            gCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAGGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGTCCTCAGGTTCCTGATCCGGCACCGGGGGTACCACt                                                                                >  2:543354/1‑143 (MQ=255)                                                            gCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACt                                                                                >  2:535866/1‑143 (MQ=255)                                                                 gcgGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGATCCTGATCCGCCACCGGGGGTACCACTAcccc                                                                           >  2:839048/1‑143 (MQ=255)                                                                  cgGCTGGAGGAGTGCGTCTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAAGAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCt                                                                          <  2:503907/143‑1 (MQ=255)                                                                   ggCTTGAGGAGTGCGTTTGATAATCATGGTATACGCTGATTCGTTACGGTGAGAATAGCAAAAGGACAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGGTCCTGATCCGGCACCGGGGGTACCACCACGCCtt                                                                         >  2:924713/1‑143 (MQ=255)                                                                    gCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCGGAGGTTCCTGATCCGGTCGCGGGGGTACCCCTACGCGTTc                                                                        >  1:509273/1‑143 (MQ=255)                                                                      ttGAGGAGTGCGTTTGACAAGCAGGGGAACCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCAc                                                                      <  1:113874/143‑1 (MQ=255)                                                                          ggagTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGCACCACTACGCCTTCACgcgg                                                                  >  1:135468/1‑143 (MQ=255)                                                                             gcgCGTTTGACAATCATGCTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGCGCAGAGCCAGTGGCCCTGCCCTTATCAGTTCTCAGGCCCCAGGTTCCTGATCCGGTCCCGGGGGTACCACTACGCCTTCACGCGgcgc                                                               <  1:458623/141‑1 (MQ=255)                                                                               gCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCtt                                                             >  1:16116/1‑143 (MQ=255)                                                                                   tgcaTAATCATCGCACCCGCTAATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAgg                                                         <  1:736948/140‑1 (MQ=255)                                                                                          tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAGGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGAGCTTCAGGATTCGgc                                                  >  2:881575/1‑143 (MQ=255)                                                                                          tCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTGAGGTTCCTGATCCGGTACCGGGGGTACCACTAGGCCTTCACGCGGAGCTTCAGGATTCGgc                                                  >  2:881660/1‑143 (MQ=255)                                                                                               gTATCCGCTGATGCGTTACGGTGAGAATAGCAAAAGGGCAGAGCTAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTgg                                             <  1:888163/143‑1 (MQ=255)                                                                                                      ttGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCGGGGCGATtc                                      >  1:212640/2‑143 (MQ=255)                                                                                                        gATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcat                                    <  1:304249/143‑1 (MQ=255)                                                                                                              ttACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGCTTCGGCGCTGGCAGATTCATCAACtt                              >  1:1010464/1‑143 (MQ=255)                                                                                                               tACGGTGGGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACGACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTc                             <  2:108848/143‑1 (MQ=255)                                                                                                                       gaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTGCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGagt                     >  1:79050/1‑141 (MQ=255)                                                                                                                           aGCATAATGTCAGATCCAGTGGCCGTGCCTGTATCAGTTATCAGGCCGCAGGTTCGTGAGCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGAGTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTc                 <  2:88918/143‑1 (MQ=255)                                                                                                                           aGCAGACGGGCAGAGCCAGTCGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTc                 <  2:410597/143‑1 (MQ=255)                                                                                                                           aGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTc                 <  1:982719/143‑1 (MQ=255)                                                                                                                            tgataaGGGCAGGGCCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg                >  1:254274/5‑143 (MQ=255)                                                                                                                            gCAAAGGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACGACGCCTTCACGCGGCGCGTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg                <  2:230190/143‑1 (MQ=255)                                                                                                                                aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGTTACCGGGGGTACCACTACGCCTTCACGCGGCGCGTCAGGCTTCTGCGCCGGCACATTCATCCACTTCGCCACAATGcctcgcgc            >  1:1049728/1‑136 (MQ=255)                                                                                                                                aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAg            >  2:1003030/1‑143 (MQ=255)                                                                                                                                   ggCAGGGCCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTAACGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCGTCAGCTTCGGCGGAGTGGTCGCCCGttt         >  1:669862/1‑143 (MQ=255)                                                                                                                                      agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGa                                                        >  2:412950/1‑93 (MQ=255)                                                                                                                                      agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGa                                                        <  1:412950/93‑1 (MQ=255)                                                                                                                                      agagCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg      <  2:873334/143‑1 (MQ=255)                                                                                                                                        agCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgc    <  1:820426/143‑1 (MQ=255)                                                                                                                                          ccAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcgc  <  1:556201/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                            GCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGC  >  NC_000913/2432850‑2433129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑