| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,621,591 | A→T | 8.2% | Q132L (CAA→CTA) | purM → | phosphoribosylformylglycinamide cyclo‑ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,621,591 | 0 | A | T | 8.2% | 138.0 / 3.7 | 51 | Q132L (CAA→CTA) | purM | phosphoribosylformylglycinamide cyclo‑ligase |
| Reads supporting (aligned to +/- strand): ref base A (26/19); new base T (2/2); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACG > NC_000913/2621450‑2621728 | ttGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGGTGTCTGCaa > 2:1051178/1‑143 (MQ=255) tGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAGAGTGGATGTTGATACCGATT‑GGCGGTGGAGCAGCGGCATTGCGGAAGGTTGTCTGCAAt > 2:216842/1‑143 (MQ=255) aTCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCa > 1:719569/1‑143 (MQ=255) tCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCCATCAg > 2:52683/1‑143 (MQ=255) ggggcggcATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCCGTGTTTCCCCGACTATTACGGCACAGAAAAACCGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGc < 1:149401/136‑1 (MQ=255) tgtgCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGTGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACtg < 2:545386/143‑1 (MQ=255) cGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCACTCAGGCTGTTAACtggtgg > 2:759335/1‑143 (MQ=255) ggtAAGGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTggtggg < 1:202865/141‑1 (MQ=255) tGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAACACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCGATCAGCCTGTGCCCTGGTGGGGGGCg > 2:167653/1‑143 (MQ=255) tGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAACCTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCg < 2:114655/143‑1 (MQ=255) gggggtgCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTGCGCAACCGGAAAACTGGAAGACGTTTTCTATGCGTCTGT‑GATCAGCCTACGTCTGGTGGGGGGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACgg < 1:772438/140‑1 (MQ=255) gtggtgCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAGCGGc > 1:18282/1‑143 (MQ=255) tgCACGGTGCCGACTCGCTGTTTTTCCTCGCCTATTACGCGTCCGCAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGCCATTGCGGAAGGTTTTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGaa < 2:42213/143‑1 (MQ=255) tgCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGaa < 1:2904/143‑1 (MQ=255) tGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGACACGGCAGAAAGGCCgg > 2:896267/1‑143 (MQ=255) gCAGAGCCGCTGTGTTTCCGCGCCGATTACGCAAGCGGAAAACTGGAGGTTGATACGGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCggg < 1:432508/143‑1 (MQ=255) gagCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGAt > 1:884848/1‑143 (MQ=255) gCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGGGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAGATGCCGGGGATgg > 1:103222/1‑142 (MQ=255) cGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GCTCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGGACCGCCAGGTAGGCCGGGGCTGTAt > 1:811242/1‑143 (MQ=255) gCTGTTTTTCCTCGACTCATACGCCCCCGGAGAAGTGGAAAATTAAACCTTGTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATc < 1:1039807/143‑1 (MQ=255) gCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCGGTGACATTGCGGAAGGTTGTCTGCAACCCACCCGCTCGCTGGTGGGTGGCTTAACGGCAGAAATGCCGGGGATGTATc > 2:626239/1‑143 (MQ=255) gTTTTTCCTCGACTATTACGCAACCGGGATACTTGATTTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGGGGGTGGCGAAACGGCAGACATGCCGGGGATGTATCACg > 2:640521/1‑143 (MQ=255) ttgtgccTCGACTCTTACGCATACCGAAATCTGGATGTTGAGTCCGTGGCCGCGTG‑GGTCAGCCGCCTTGCGTATGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACgg < 2:854161/138‑1 (MQ=255) tCCTCGCCTATTACTCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAg < 1:674192/143‑1 (MQ=255) tCCTCGACTATTGCGCAACCGGAAAACTGGATGTTGAGACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAg < 1:141203/143‑1 (MQ=255) tCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGAt > 1:701824/1‑143 (MQ=255) tCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGAt > 2:599485/1‑143 (MQ=255) cGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCACTCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGtt > 2:1001593/1‑143 (MQ=255) cTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGt > 2:348890/1‑143 (MQ=255) tGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCTATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc > 2:234230/1‑118 (MQ=255) tGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCTATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:226962/118‑1 (MQ=255) tGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCTATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc < 1:234230/118‑1 (MQ=255) tGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCTATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTAc > 2:226962/1‑118 (MQ=255) ttGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACgg < 2:399923/81‑1 (MQ=255) ttGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACgg > 1:399923/1‑81 (MQ=255) ttGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTg < 1:467825/143‑1 (MQ=255) tGACACCGCTTCAGCGGT‑GAGCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTgg < 1:478906/143‑1 (MQ=255) aTACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTa > 2:151551/1‑143 (MQ=255) tACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAg > 2:299406/1‑143 (MQ=255) aCCGCTTCAGCGGT‑GATCAGCGGCATTGCGGACGGTTGTCTGCAATCCGGCCGTTCACTGGTGGGTGGCGAAACGGCAGACATGCCGGGGGTGTATCCCGGGGAGGATTACGCTGTCGCGGGTTTCTGCGTTGGCGCGGGAgc > 2:478066/1‑142 (MQ=255) cGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGaaa > 1:10089/1‑143 (MQ=255) cGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTCTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTGTCACGGTGCGCATTACCATGTCGCGGGTTTCTGCGTGGGCGTGGTAGaga > 2:597448/1‑141 (MQ=255) gcgtCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGaaaa < 1:696258/140‑1 (MQ=255) gCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGAc > 1:246120/1‑143 (MQ=255) gCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACGGGTGGGTGGCGAACCGGCAGAAATGCCGTGGATGTCTCGCGGTGAGGATTACGATGTCGCGGGTTGCTGCGTTGGCGAGGGAGCAAAATCCGAACGCCTCGAc > 2:638112/1‑143 (MQ=255) cagCATTGCGTAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGATGCTGCGTTGGCGTGGTAGAAAAATCAGAACTCATCGACg > 2:183315/3‑143 (MQ=255) cggtGCGGAAGGTGGACGGCAAGCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGct < 1:617289/140‑1 (MQ=255) aTTGCGGAAGGGGGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGCAATGCCGGGGATGTGTCACGGTGAGGATTACGATGTCGCGGGATTCTGCGTTGACGTGAGAGAAAAATCGGACACCCACGACAGctc > 2:520560/1‑143 (MQ=255) aGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGTGGATGTATGACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGGAGAAAAATCAGAAATCATCGACGGCACTAAAGTCa > 2:225246/1‑143 (MQ=255) aGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGGGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCa > 2:1016298/1‑143 (MQ=255) aGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCa < 1:889977/143‑1 (MQ=255) tCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACg < 1:446754/143‑1 (MQ=255) | TTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTCCTCGACTATTACGCAACCGGAAAACTGGATGTTGATACCGCTTCAGCGGT‑GATCAGCGGCATTGCGGAAGGTTGTCTGCAATCAGGCTGTTCACTGGTGGGTGGCGAAACGGCAGAAATGCCGGGGATGTATCACGGTGAGGATTACGATGTCGCGGGTTTCTGCGTTGGCGTGGTAGAAAAATCAGAAATCATCGACGGCTCTAAAGTCAGCGACG > NC_000913/2621450‑2621728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |