Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG100.0% 127.0 / NA 40V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/21);  total (19/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA  >  NC_000913/2661834‑2662105
                                                                                                                                       |                                                                                                                                        
ccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCa                                                                                                                                   <  2:393444/143‑1 (MQ=255)
  tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt                                                                                                                                 >  1:644529/1‑143 (MQ=255)
      aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCCCGTACGCTGGAAAGCAGACCATTTTTa                                                                                                                             <  1:281226/143‑1 (MQ=255)
      aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTa                                                                                                                             <  1:784433/143‑1 (MQ=255)
         cgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGc                                                                                                                          >  2:44429/1‑143 (MQ=255)
                aCCCTGACAACGGGTGGCATCGACAGATTCGTCAACGGCGCTAATTACTTCGCCCACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGCAAAGCAGACCATCTTTACCAAGACgac                                                                                                                   >  2:642956/1‑141 (MQ=255)
                 cccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCCGGACAGCAGACCATTTTTACGCAGGCggcc                                                                                                                  >  2:902740/1‑141 (MQ=255)
                  ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCCCGTACGCTGGAAAGCAGACCATTCTCACGCCGACGGGaa                                                                                                                 >  2:819473/1‑143 (MQ=255)
                        caCGGGTGGAATCTACAGATTCGTCAACGGCGGTGATTACTTCGCGAGCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCGTTTTTACGCAGACGGGAAAACAgt                                                                                                           <  2:800445/142‑1 (MQ=255)
                         aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                           <  2:786581/126‑1 (MQ=255)
                         aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                           >  1:786581/1‑126 (MQ=255)
                            ggTGGCATCTCCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgttgtt                                                                                                       <  2:386937/143‑1 (MQ=255)
                            ggTGGCACCTACGGATTCGTCACCGGCGCTAATTACTCCGCCAATGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCAgttgtt                                                                                                       <  2:805205/143‑1 (MQ=255)
                                     tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGGAAGCAGACCATTTTTACGCCGACGGGAAAACAGTTGTTACCGCTAAg                                                                                              >  2:757481/1‑143 (MQ=255)
                                        aggttCCTCAACGGCGCTAAGTACGTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAGCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                           <  2:29266/140‑1 (MQ=255)
                                        aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                           >  1:136155/1‑143 (MQ=255)
                                            tCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa                                                                                       <  1:777705/143‑1 (MQ=255)
                                               tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                    <  1:551883/143‑1 (MQ=255)
                                                  aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc                                                                                 >  1:678256/1‑143 (MQ=255)
                                                    ggCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                               <  1:44429/143‑1 (MQ=255)
                                                            ctactaggCCAGCGGCGGTGGTGCTGGCATGTGTGCCTAACAGATAACCGCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                       <  2:956812/136‑1 (MQ=255)
                                                               ctgcGCCAACGGCGATGCTGGTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa                                                                    <  1:902740/140‑1 (MQ=255)
                                                                ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                   >  1:843114/1‑143 (MQ=255)
                                                                     cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAGCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                              <  2:56131/143‑1 (MQ=255)
                                                                           cGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAAc                                                        >  1:550142/1‑143 (MQ=255)
                                                                                            ttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTc                                       >  1:739966/1‑143 (MQ=255)
                                                                                             tGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTCTACCCGATGCGAAGGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                      <  2:469628/143‑1 (MQ=255)
                                                                                               ccgaaCAGATAACCACCGCCGGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                    <  1:479949/140‑1 (MQ=255)
                                                                                               ccTAACAGATAACCACCGCATGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                    <  2:965990/143‑1 (MQ=255)
                                                                                                 tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                  <  1:819473/143‑1 (MQ=255)
                                                                                                 tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTCTTACGCAGACGGGAAAACAGTTGTTCCGGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCAGGCCCGCCTCagagc                                  >  2:767633/1‑142 (MQ=255)
                                                                                                  aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCGGCCAACGGTACCGGGCCCGCTTCAGAGtt                                 <  2:541359/143‑1 (MQ=255)
                                                                                                           ccaccGCCAGGTCCACGTGCGCTGGAAAGCAGACCAATTTTACGCAGGCGGGAAAACAGTCGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                        <  1:190873/143‑1 (MQ=255)
                                                                                                             accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTGCCGAGCTGAGCGCAGCgc                      >  2:461021/1‑142 (MQ=255)
                                                                                                              ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                     >  2:135932/1‑143 (MQ=255)
                                                                                                                     gcccACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt              <  2:924588/141‑1 (MQ=255)
                                                                                                                         aCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTAACGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCgg          >  2:439985/1‑143 (MQ=255)
                                                                                                                                cTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGc   >  1:1023127/1‑143 (MQ=255)
                                                                                                                                 tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa  >  2:123402/1‑143 (MQ=255)
                                                                                                                                 tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGCTCACGGCa  >  2:621513/1‑143 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA  >  NC_000913/2661834‑2662105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑