Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,929,722 G→A 8.5% V49V (GTG→GTA)  sdaB → L‑serine deaminase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,929,7220GA 8.5% 122.1 / 2.9 49V49V (GTG→GTA) sdaBL‑serine deaminase II
Reads supporting (aligned to +/- strand):  ref base G (18/26);  new base A (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

TAGCGTATTCGATATTTTCAAAATCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTG  >  NC_000913/2929581‑2929863
                                                                                                                                             |                                                                                                                                             
tAGCGTATTCGATATTTTCAAAATCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACgtgt                                                                                                                                              <  2:964421/143‑1 (MQ=255)
     tATTCGATATTTTCAAAATCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGAAGTGTACGgc                                                                                                                                         >  1:360736/1‑143 (MQ=255)
                ttCAAAAGCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGctctctct                                                                                                                              <  1:216273/143‑1 (MQ=255)
                    aaaTCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGAcc                                                                                                                          <  2:541004/143‑1 (MQ=255)
                        cGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTATACGGCTCGCTCTCTCTGACCGGTa                                                                                                                      >  2:437689/1‑143 (MQ=255)
                        cGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTATACGGCTCGCTCTCTCTGACCGGTa                                                                                                                      >  2:436026/1‑143 (MQ=255)
                          gCATTGGCCCTTCCAGTTCTCATACCGTTGGCCAGAAAAGATCGGGGAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTaaa                                                                                                                    <  2:741767/143‑1 (MQ=255)
                          gCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGTTCGCTCGCGCTGACCGGTaaa                                                                                                                    >  2:500445/1‑143 (MQ=255)
                          gCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTaaa                                                                                                                    <  1:924937/143‑1 (MQ=255)
                             ttGGCCCTGCCACTTCTCATACCGGTGCACCAATGAAAGCGGGTAAACAATTTCCCGACGATCTGATTGCCCGTAACCTTCTTAAAGACGTGGCCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGc                                                                                                                 <  2:387821/143‑1 (MQ=255)
                                          ttCTCATACCGTTGGACCAATGAAGGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACTGCTCGCTCTGTGTGACCGATAACGGCCACCACACTGcta                                                                                                    >  2:167158/1‑143 (MQ=255)
                                           tctcATACCGTTGGACCAATTAAAGCGGGTAAACAAGTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGatat                                                                                                   <  2:214270/143‑1 (MQ=255)
                                            ctcATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATc                                                                                                  >  2:738550/1‑143 (MQ=255)
                                               aTACCGTTGGCCCACTGAAAGCGGGTAAACAATTCACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGcc                                                                                               <  1:122401/143‑1 (MQ=255)
                                               aTACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTCAAGGCCCCCACACTGATCTCGcc                                                                                               >  2:826451/1‑143 (MQ=255)
                                               aTACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGcc                                                                                               >  2:504182/1‑143 (MQ=255)
                                                 aCCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATGGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCat                                                                                             <  1:517820/143‑1 (MQ=255)
                                                  ccGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCggcctt                                                                                            >  1:927524/1‑138 (MQ=255)
                                                   cGTTGGAACAAGGTAAGCGGGTAAACAATTGCCCGACGATCTGATTGCCTGTAACCTGCTTAAAGACGTGCCCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCatta                                                                                           <  1:63759/143‑1 (MQ=255)
                                                      tGGACCAATGAAGGCGGGTAAACAATTGCCCGACGATCGGATTGCCCGTAACCTGCGTAAAGACGTGGCCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCAttatta                                                                                        <  2:337914/143‑1 (MQ=255)
                                                       ggACCAATAAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCCTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATtattat                                                                                       <  1:256442/143‑1 (MQ=255)
                                                        gACCAATGAAGGCGGGTAAACAATGTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATg                                                                                      <  2:581004/143‑1 (MQ=255)
                                                           cAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACATGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTCTTATGGGc                                                                                   >  1:530698/1‑143 (MQ=255)
                                                             aTGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCAGTCTTATGGGccc                                                                                 >  2:723084/1‑142 (MQ=255)
                                                              tGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTGTTATGGGCCTg                                                                                >  1:529549/1‑143 (MQ=255)
                                                               gAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTgg                                                                               <  2:181527/143‑1 (MQ=255)
                                                                   gCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTGAAGACGGGGCCCGGATGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCggg                                                                           <  1:144356/143‑1 (MQ=39)
                                                                   gCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGCCCGGTAACAGCCACCACACTGATATCGCCCTTATTATGGGCCTGGCggg                                                                           >  2:629447/1‑143 (MQ=255)
                                                                         aaaCAATTGACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCt                                                                     <  1:85968/143‑1 (MQ=255)
                                                                           aCAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGc                                                                   >  1:85014/1‑143 (MQ=255)
                                                                           aCAACTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGc                                                                   <  1:926894/143‑1 (MQ=255)
                                                                            cAATTTACCGACGATCTGATTGCCCGACACCCGCTTAAAAACGTGACCCGCGTGGTGGTTGACGTCTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGCTATCGCCACTATCATGGGCCTGGCGCGAAACCTGcc                                                                  >  2:641537/1‑143 (MQ=255)
                                                                             actctACCGACCGTGTGATTGCCCGTAGCCGTCTCAAAGCCGTGCCCCGCGTGGGGGGTGACGCGTACGGCCCGCTCTCTCTGACCGGGAACGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCg                                                                 <  2:870976/139‑1 (MQ=255)
                                                                             aaTTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCg                                                                 <  1:738550/143‑1 (MQ=255)
                                                                              atgtACCGACGATCGGATTGCCCGTAGCCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCAGGTTACCCGccag                                                                <  1:749405/140‑3 (MQ=38)
                                                                                      acgaTCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGGAACCTGCCGGATACCGTg                                                        >  1:48684/1‑143 (MQ=255)
                                                                                        gaTCTGATTGCCCGTAACCTGCTGAAGGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGa                                                      <  1:962580/143‑1 (MQ=255)
                                                                                          tCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCACGTGGTGGTTGACGTGTACGGCACGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGAAACCCGCCGTATACAGTGGcta                                                    >  2:518576/1‑143 (MQ=255)
                                                                                              aTTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGa                                                >  1:908562/1‑143 (MQ=255)
                                                                                               ttGCCCGTAACCTGCTCAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCGCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGAt                                               <  1:250081/143‑1 (MQ=255)
                                                                                                tgaccGTAACCTTCTTAAAGACGTGACCCGCGTGGTTGTTGACGTGTACCGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGAtt                                              <  1:1003952/140‑1 (MQ=255)
                                                                                                    cgtctgcTGCTTCAAGCCGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCAGCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCAt                                          <  2:237104/137‑1 (MQ=39)
                                                                                                    cGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCAt                                          <  2:731705/143‑1 (MQ=255)
                                                                                                       aaCCTGGTTAACGTCGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATccc                                       <  2:866003/143‑1 (MQ=255)
                                                                                                         ccTGCGTACAGGCGGGAGCCGCGGGGCGCTTGACGCGTCGGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTAGGGGCCGGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCa                                     <  1:167158/143‑1 (MQ=255)
                                                                                                                     cGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTCACCTGTCGGATACCGTGGGTATCGATTCCATCCCCCGTCTTTTTCAgg                         >  2:258965/1‑143 (MQ=255)
                                                                                                                           ccGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGa                   >  1:70623/1‑143 (MQ=255)
                                                                                                                                  gtggtTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTCATTCAGGATGTGacacctca            >  2:392323/1‑136 (MQ=255)
                                                                                                                                   tggtTGACGTATACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCAt           <  1:436026/143‑1 (MQ=255)
                                                                                                                                   tggtTGACGTATACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCAt           <  1:437689/143‑1 (MQ=255)
                                                                                                                                            tgtACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGGTGTGAATACTCATGGTCGGCTg  >  1:685302/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                             
TAGCGTATTCGATATTTTCAAAATCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAAACAATTTACCGACGATCTGATTGCCCGTAACCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTG  >  NC_000913/2929581‑2929863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑