BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,632	T→A	100%	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	141.3 / NA	49	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (24/25); total (24/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041512‑3041770

gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAg                                                                                                                      >  1:835621/1‑143 (MQ=255)

     aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGAGACCATCGCGCCAGACGACGAGCgcgt                                                                                                                 >  2:293640/1‑143 (MQ=255)

     aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                                 >  2:791744/1‑143 (MQ=255)

           aGCACTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTgctgct                                                                                                           <  1:699533/143‑1 (MQ=255)

             cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                         <  1:692837/143‑1 (MQ=255)

                tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                      >  1:319123/1‑143 (MQ=255)

                tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                      >  1:670696/1‑143 (MQ=255)

                    cggcggTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                                  <  2:758268/138‑1 (MQ=255)

                       cTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                                                               <  2:108945/143‑1 (MQ=255)

                       cTGTTCCGCGACGACCGTGTCTTTGCATAGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                                                               <  1:652188/143‑1 (MQ=255)

                         gTTCCGCGACGGCGATGGCTTGGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGAGCCGGTTCTCTATCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                             <  2:581879/143‑1 (MQ=255)

                            ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                          <  1:35207/143‑1 (MQ=255)

                              cccactgCGATGTCTTTGCATGGATTGACCGCCGCACCTTGCGTGAACCGCAGCTGACTGAACTGCAACAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                        <  1:94/137‑1 (MQ=255)

                                gACGGCGATGGCTTTGTGTGGAGTGAACGGCGCCGCGGGCGTGATCCGCAACTGACTGAACTGTAAGAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGTCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                      <  2:266785/143‑1 (MQ=255)

                                    gCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgc                                                                                  <  1:674771/143‑1 (MQ=255)

                                     cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTCCGgcgcgcg                                                                                 >  2:946778/1‑143 (MQ=255)

                                       aTGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGGCTTTCAGgcgcgggcg                                                                               >  2:232899/1‑142 (MQ=255)

                                           cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAACTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGCGCCAGTCTTCAGGCGcgcgccgcgc                                                                           >  2:502907/1‑143 (MQ=255)

                                            tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGGGTGCTGCTTGGTGTTGCCGGCATACAAGCGcgcgccgccgc                                                                          >  2:94924/1‑140 (MQ=255)

                                             ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGTGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                         <  1:258634/143‑1 (MQ=255)

                                             ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                         <  1:421647/143‑1 (MQ=255)

                                               gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTGTTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                                                                       <  1:530938/143‑1 (MQ=255)

                                                 aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTCAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCCTGGTGTTGCCGGTTTTCACGCGCGCGCCGCGCTGGCaa                                                                     >  2:1035869/1‑143 (MQ=255)

                                                  tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                                                                    <  2:77482/143‑1 (MQ=255)

                                                  tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                                                                    >  2:947712/1‑143 (MQ=255)

                                                    gATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGGCGGTTTTCAGGCGCGCGCCGCGCTGGCAGAtc                                                                  >  1:512982/1‑143 (MQ=255)

                                                      ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGACGCGAGCCGCGCGGGCAAGtctc                                                                >  1:941649/1‑143 (MQ=255)

                                                        gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAACTCTCtt                                                              >  1:722927/1‑143 (MQ=255)

                                                         aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                             >  1:672552/1‑143 (MQ=255)

                                                          aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                            <  1:967696/143‑1 (MQ=255)

                                                           cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg                                                           <  1:1028665/143‑1 (MQ=255)

                                                           cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCCAATCTCTTTAg                                                           >  2:100489/1‑143 (MQ=255)

                                                               gcAGCGTGCGTGAACCGCAGCTGACTGAACTGGAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGNGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                       <  1:850403/143‑1 (MQ=255)

                                                                    gttcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGcc                                                  <  1:200399/140‑1 (MQ=255)

                                                                      gcgttAACCTCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACCATCGCGCCAGCCGACGGGCGTGGGCGGCTTGGTGTTGCCGGTGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                                <  2:234623/143‑1 (MQ=255)

                                                                          gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGCCGCGCGCCGCGCTGGCAAAGCTCTGTAGCGAACTGCCTTCgaa                                            >  1:479389/1‑143 (MQ=255)

                                                                              cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa                                        <  1:847249/143‑1 (MQ=255)

                                                                                     gactgaGCTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGGGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                                 <  2:947032/143‑1 (MQ=255)

                                                                                       ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                               >  1:933615/1‑143 (MQ=255)

                                                                                        tgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAAACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCCGGCACATCTCTTTAGCGAACTGCCATCGCAAGTATACCAGGtcg                              >  2:340507/1‑141 (MQ=255)

                                                                                          aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGTAAGAAAAACAGGAAgca                            >  1:654652/1‑141 (MQ=255)

                                                                                             tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTATAGCGAACTGCCTTCGAAAGAAATACAGGTAGGCaaa                         >  1:137828/1‑143 (MQ=255)

                                                                                              gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag                        <  2:804236/143‑1 (MQ=255)

                                                                                               aaaagaTATGGGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga                       <  1:470490/143‑1 (MQ=255)

                                                                                                       tGCGGTAATCTCGAAAGAGACCATCGCGGCAGACGGCGAGCGTGTGCGGGTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCACTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa               <  2:569870/143‑1 (MQ=255)

                                                                                                              ttCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt        >  1:1003913/1‑143 (MQ=255)

                                                                                                               cctctAAAGAGACAATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAACTCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg       <  1:230274/142‑1 (MQ=255)

                                                                                                                   tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt   >  2:572622/1‑143 (MQ=255)

                                                                                                                    aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCGTTAGCGTACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCGATg  >  1:93575/1‑143 (MQ=255)

GTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041512‑3041770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`