| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,156,590 | C→A | 7.4% | intergenic (+72/‑33) | yqhD → / → dkgA | NADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,156,590 | 0 | C | A | 7.4% | 156.9 / 4.5 | 62 | intergenic (+72/‑33) | yqhD/dkgA | NADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA |
| Reads supporting (aligned to +/- strand): ref base C (33/24); new base A (2/3); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01 | |||||||||||
CCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAA > NC_000913/3156452‑3156729 | ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGcc > 2:84698/1‑142 (MQ=255)ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTCTCGTTTGCGGGCCTTTCGTCCAGACTGAAGATCACAACACCTCACCGGAGCCAGca > 2:345443/1‑142 (MQ=255) agtGGGCGAAATTCATGACATTATTTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTGTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg < 1:829024/141‑1 (MQ=255) aCTTGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCg < 1:22661/143‑1 (MQ=255) tGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGt < 1:649771/143‑1 (MQ=255) ggCGACAATCATGCCGTTACGTCGGATGTCCGCCGCCGTATACTCTCTGTCGCCCGCTAAGCTTTTTACGCCTCAAACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGa < 2:1021028/143‑1 (MQ=255) gCGAAAATCATGACATTACGGTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTGCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAg < 2:855597/143‑1 (MQ=255) aactCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggtgt < 1:483086/140‑5 (MQ=255) tCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGCGCTCatac > 2:384461/1‑142 (MQ=255) cATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGGGAGTTCAtata > 1:951109/1‑143 (MQ=255) tGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa < 2:532380/143‑1 (MQ=255) tGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa < 1:232730/143‑1 (MQ=255) cATTACGTTGTATGTCAGCCTCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCTTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga < 1:884630/143‑1 (MQ=255) cATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga < 1:1003775/143‑1 (MQ=255) aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggag > 1:519900/1‑143 (MQ=255) gTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACg > 1:587038/1‑143 (MQ=255) atttCAGCCGCCGTACATACGAGGCCGCCCGTTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCAGTTCGTCCAGAGTTAACTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg < 2:451975/140‑1 (MQ=255) tCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTa > 1:890121/1‑143 (MQ=255) cAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTaa < 2:890829/143‑1 (MQ=255) ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGTCATTTCGTCCGGACCTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGGGAGGTCATATAAAGGAGGAACGTAGGGCTGATca > 2:778267/1‑142 (MQ=255) tataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtta > 1:998351/1‑143 (MQ=255) taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCGAATCCAACCGttatt > 1:225480/1‑143 (MQ=255) aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATACAGGAGGAACGTATGGCTAATCCGACCGttatta > 1:779863/1‑143 (MQ=255) cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa > 1:1017708/1‑143 (MQ=255) ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCCTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATCAAGGAGGAACGTATGGCTAATCCAACCGTTATTCAGCtgc > 2:589968/1‑141 (MQ=255) cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa > 1:720825/1‑143 (MQ=255) gcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAg > 1:13711/1‑143 (MQ=255) gcTAAGCTTTTTACGCCTCAAACTTTCGTTGTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg < 1:179112/143‑1 (MQ=255) gcTAAGCTCTTTCCGCCTCAAACTCTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGAGAGTTCATATAACGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg < 1:983424/143‑1 (MQ=255) tAAGCTTTTTACGCCTCAAACTTTCGGTTTCGGGCATTTCGTCCAGACTTAACTTCACAACACCTCACCAGAGCCTGCTCCGGTGAGTTCATATAAACGAGGAACGTATGGCTAATACACACGTACTTGAGCTACAGGaaagc > 2:42108/1‑139 (MQ=255) gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCCTATAAAGCAGGCACGTATGGGTAATCCACCCGCTATTAGACTGGAGGATGGCGAt > 2:501785/1‑143 (MQ=255) gCTTTTGACGCCTCAAACTTTCGTTTTCGGGCATGTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATATAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAt < 2:548183/143‑1 (MQ=255) tttACGCCTCAAGCTTTGGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCa < 2:711410/143‑1 (MQ=255) tACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg < 1:647802/143‑1 (MQ=255) cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGCGAGTTCATATAAAGGCGGCACGTACGGCTCATCCAACCGTTCTTAAGCCCCAGGCTCGCACTGCCATGCCCCa > 2:656855/1‑143 (MQ=255) cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACATCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTGCAGGATGGCAATGTCATGCcaca > 2:51306/1‑143 (MQ=255) tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCACTGTCATGCCCCAg > 1:183356/1‑143 (MQ=255) ccaaCTCTCGTTTTCGGGCATTTCGCCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc < 1:781243/141‑1 (MQ=255) cAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc > 1:1023272/1‑143 (MQ=255) tttCGTGTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa < 1:408401/143‑1 (MQ=255) ttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc > 2:280454/1‑143 (MQ=255) cGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGAGACTGGGCggc > 1:795255/1‑141 (MQ=255) tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaa < 1:540188/143‑1 (MQ=255) tCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagc < 1:946826/143‑1 (MQ=255) ccAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGt > 2:375961/1‑143 (MQ=255) cgtAAGTTCACACCACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCGCCAGCTGGGACTGGGCGGCTGGCAAGCAAGTAATGa < 2:873532/141‑1 (MQ=255) ttAAGTTCACAACACCTCCGCTGAGCCTGCCCCGATCAGATCAGAGAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAg < 2:741380/143‑1 (MQ=255) ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAg > 1:638820/1‑143 (MQ=255) gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAg < 1:84698/143‑1 (MQ=255) aacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTACTCCAACCGTTATTAAGCTACAGGATGGCAATGTCGTGCCCCAGCTGGGACTGGGCGTCTGGCAAGGAAGTAATGAGGAAGTAATCa > 1:380314/1‑143 (MQ=255) aacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCa > 2:784199/1‑143 (MQ=255) tCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa > 1:1429/1‑143 (MQ=255) tCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGGAATCCACGccc > 2:804946/1‑142 (MQ=255) cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGCACTGGGCGTCTGGCAAGCAAGTCATGAGGAAGGAATCACCGCCAt > 2:501962/1‑143 (MQ=255) aCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTCCTGGATTGCAATGTCATGCCCCAGCTGGGACGGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAtt < 1:345443/143‑1 (MQ=255) aCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAtt > 1:451957/1‑143 (MQ=255) aCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAtt > 1:110055/1‑143 (MQ=255) aCCGGAGCAGGCTCCGGTGAGTTCCTATAAAGGAAGAACGTATGGCTAATCCAACCGTTCTTAAGCTCCCGCATGGCAATGCCATGCCCCAGCTGGGACCGGGCGTCTGGCAAGCCAGTAATGAGGCAGTAATCACCGCCagt > 2:198669/1‑141 (MQ=255) ccGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAAATGATTGCAATGTCATGCCCCAGCTGGGACTAGGCGTCTGACAAGCACGTAATGAGGAAGGAAACACCGCCCtta > 2:264474/1‑142 (MQ=255) gAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa < 2:779863/143‑1 (MQ=255) aGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaa < 1:784199/143‑1 (MQ=255) aGCCTGCTCCGGAGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCCAGTAATGAGGAAGTAATCACCACCATTCaaac > 2:534408/1‑142 (MQ=255) | CCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAA > NC_000913/3156452‑3156729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |