Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,142 A→T 7.8% M106L (ATG→TTG)  yhcG → DUF1016 domain‑containing protein YhcG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1420AT 7.8% 177.9 / 3.6 64M106L (ATG→TTG) yhcGDUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand):  ref base A (23/35);  new base T (3/2);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAA  >  NC_000913/3368000‑3368279
                                                                                                                                              |                                                                                                                                          
cAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACaa                                                                                                                                            <  2:304530/143‑1 (MQ=255)
 aGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAAt                                                                                                                                           >  2:246727/1‑143 (MQ=255)
     cTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCt                                                                                                                                       <  1:343653/143‑1 (MQ=255)
       gCGTATGGTGCGCAGCTAATCAAGCGGCTATCAAAGGATTGATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAACAACTTACGCCAAATGAGGCTGTTTTACCTCTTGTTTCAACATGTTGAAATTCACCAGACATTGTCTgg                                                                                                                                     <  2:588197/143‑1 (MQ=255)
        gggaTGGTGCGCAGCGAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACATTGTCTggt                                                                                                                                    <  2:206411/140‑2 (MQ=255)
        cGTATGGTGCGCAGCGAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGAAAAAAACTTACTAACACAGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGc                                                                                                                                    <  2:1025705/143‑1 (MQ=255)
               tccgcAGCTAATCAAGCGACTACCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa                                                                                                                             <  1:360504/141‑1 (MQ=255)
                        aaTCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTgg                                                                                                                    <  1:15066/143‑1 (MQ=255)
                         aTCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTggg                                                                                                                   <  2:647796/143‑1 (MQ=255)
                         aTCAAGCGACTATCAAAGGATTTATGGCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTggg                                                                                                                   <  1:415055/143‑1 (MQ=255)
                          tCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTGTTGCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGa                                                                                                                  <  1:209619/143‑1 (MQ=255)
                               cGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTAGGAATTcc                                                                                                             >  2:214369/1‑143 (MQ=255)
                                gACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                            <  1:146202/143‑1 (MQ=255)
                                 aCTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                            >  2:455392/1‑142 (MQ=255)
                                 aCTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                            <  1:455392/142‑1 (MQ=255)
                                       aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGACTTCCGCAGacac                                                                                                     >  1:79826/1‑143 (MQ=255)
                                        aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAcc                                                                                                    <  1:830824/143‑1 (MQ=255)
                                          ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGt                                                                                                  <  2:776671/143‑1 (MQ=255)
                                           gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTGTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTc                                                                                                 >  1:663039/1‑143 (MQ=255)
                                              ttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                              >  2:1311/1‑143 (MQ=255)
                                                  gTCTACGGTATAAGCGTGGATTATCGGCAAAAAACTTACGCCAAATGAGGCTTTTTTCCGTCTTTGTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                          <  2:789961/143‑1 (MQ=255)
                                                   tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAt                                                                                         >  1:615569/1‑143 (MQ=255)
                                                   tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAt                                                                                         <  1:373984/143‑1 (MQ=255)
                                                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAGCGTCTGCAGAAttg                                                                                       >  1:496971/1‑142 (MQ=255)
                                                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTc                                                                                       >  2:538011/1‑143 (MQ=255)
                                                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTc                                                                                       >  2:76168/1‑143 (MQ=255)
                                                     tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATGAGCACCATTGGGAATTCCGCGGACACCGTCTGCAGAATTc                                                                                       >  1:75601/1‑143 (MQ=255)
                                                      aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTcc                                                                                      >  2:719776/1‑143 (MQ=255)
                                                        ggTATAAGCGTGGGTTCGCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCa                                                                                    <  2:817190/143‑1 (MQ=255)
                                                          tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCAGGCTTACCTCTTTTTTCAACACGCTCAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCCCAGACACCGTCTGCAGAATTCCCATc                                                                                  >  2:637826/1‑143 (MQ=255)
                                                            tatgCGTGGTTTCTCTGCAAAGAACTGACGCCTAATGAGGCTTTTTGAGCTCTTGTTTCAACATGTTTAAAGTGATCATTCAATGTCTGGCGAAATAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                <  2:973701/140‑1 (MQ=255)
                                                            taAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAGTTCCCCTCgg                                                                                >  1:316924/1‑143 (MQ=255)
                                                               gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                             <  2:998646/143‑1 (MQ=255)
                                                                cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGCCaaa                                                                            >  1:536889/1‑143 (MQ=255)
                                                                cGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTCCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                                                            <  1:648160/143‑1 (MQ=255)
                                                                   gggTTCCCTGCAAAAAACGTACGCCAAATGTGGCTTTTTTACCTCTTTTTTCATCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGCATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAt                                                                         <  2:612897/143‑1 (MQ=255)
                                                                    ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAAGTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAtt                                                                        <  1:657/143‑1 (MQ=255)
                                                                     gtgcgcggCAAAAAACTTACGCCAAATGAGGCTTTTTGACCTCTTTTTGCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                       <  2:577091/136‑1 (MQ=255)
                                                                     gTTGTCCGCAAAAAGCTTACGCTAAATGAGGCTTTTTGACCTCTTTATTCAACATGTTGAAAGTCACCAGGCAATGTCGGGCGAATTAACACCAGTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                       <  2:383756/143‑1 (MQ=255)
                                                                     gTTCTCTTCAAAATACTTCCCCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                       <  2:820016/143‑1 (MQ=255)
                                                                              aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑Gc                                                             >  1:408554/1‑143 (MQ=255)
                                                                              aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑Gc                                                             >  1:211178/1‑143 (MQ=255)
                                                                                   aCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATGCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCt                                                        <  1:145065/143‑1 (MQ=255)
                                                                                   aCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTAAAATTCACCAGACATTTTCTGACTATTTATCACCATTGGGAATCACTCAACAACCGTCTGCATAAGTCCCCTCGGCTGAGAGTTGACAGAA‑GCTGTCt                                                        >  2:835215/1‑143 (MQ=255)
                                                                                   aCTTACGCCAAACGAGGCTTTTTTCCCTCTCTTGTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCt                                                        <  1:646726/143‑1 (MQ=255)
                                                                                     ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGGTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGACTTCCGCAGACACCGTCTGCAGACTTACCATCGGCAAAGATTTGGCAGAC‑GCTGTCtcc                                                      >  2:800709/1‑141 (MQ=255)
                                                                                         gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTATGCAGAAAAGGCAATGGCAAAGATTTGGCAGAC‑GCTCTCTGCCaaa                                                  >  2:1019594/1‑143 (MQ=255)
                                                                                          ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACCCAAATGAGTATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGC‑GTCTGCCAAAt                                                 <  1:148997/143‑1 (MQ=255)
                                                                                          ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCCGCCCCCGTCTGCAGATTTCCCATCGGCAAAGATTTGGCAGACAGC‑GTCTGCCAAAt                                                 <  1:1000325/143‑1 (MQ=255)
                                                                                            aaaTGAGGCTTTTTTACCTCTTTTTTCAAGATGTGGGGTTTTGGGAGTGAGTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATCTGGCAGAC‑GCTGTCGGCCGAATcg                                               >  1:777923/1‑142 (MQ=255)
                                                                                            aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCaactgt                                               >  2:565704/1‑139 (MQ=255)
                                                                                                     ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCGAAGGTTTGGCAGAC‑GCTGTCTGCCAGATCTTTGCCCTTg                                      >  1:65507/1‑143 (MQ=255)
                                                                                                      tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGc                                     <  1:770756/143‑1 (MQ=255)
                                                                                                         ttACCTCTTTTTTCAACATGTTTAAATTCACCAGACAATGTCTGGCCAATTAACACCATTGGGAATTCCGCACACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCa                                  <  1:779211/143‑1 (MQ=255)
                                                                                                               ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                            >  2:497163/1‑143 (MQ=255)
                                                                                                                ttttttCAACGTGTTCAAATTCACCAGGCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                           <  2:490825/143‑1 (MQ=255)
                                                                                                                ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                           <  2:241996/143‑1 (MQ=255)
                                                                                                                ttttttCAACAGGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                           <  2:722009/143‑1 (MQ=255)
                                                                                                                          aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                 <  1:719776/143‑1 (MQ=255)
                                                                                                                               gAAATTCACCAGACAATGTCTGGCGAATTAACACCATTAGGAATTCCGCAGACACCGTCTGCAGAATTACCATCAGCAACGATTTGGCAGAC‑GCTCGCTGCCAAATCTTTCCCCTTGACCAGGTCCCCATACGTCCGTTCACt            >  1:775938/1‑143 (MQ=255)
                                                                                                                                aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACtt           <  1:538011/143‑1 (MQ=255)
                                                                                                                                  aTTCGCCAGACATTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAACGATTTGGCAGAC‑GCTGTCTGCCAAATGTTTCCCCTTGCCCAGGTCAACCTCCGTCCGTGTCCTTTc         >  1:337416/1‑143 (MQ=255)
                                                                                                                                   ttCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc        <  1:961825/143‑1 (MQ=255)
                                                                                                                                      gccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCCAATCTTTCCCCTTGCACAGGTCCACATACGGCCGCTTACTATCCGtt     >  2:342054/2‑143 (MQ=255)
                                                                                                                                         aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa  <  2:17683/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
CAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAC‑GCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAA  >  NC_000913/3368000‑3368279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑