| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 97.5% | 104.9 / NA | 41 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/20); minor base C (0/1); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759119‑759389 | cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGCTCCacc > 1:662045/1‑141 (MQ=255)cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCGGAGTATATGCACATGACCAGCACGGAGGAAAAACTCTGGACCCagc > 1:551950/1‑141 (MQ=255)cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc > 1:27601/1‑143 (MQ=255) aaCGTCGGTTCACGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCg < 2:312780/143‑1 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt > 1:799284/1‑143 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt < 2:97819/143‑1 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACAGCGGCACGATCGGCGCCGAGTATATGCACATACCCGTCACCGGAGGAAAACTCGGGATCCAACCGcct > 2:754903/1‑141 (MQ=255) cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTa > 1:272370/1‑143 (MQ=255) cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCGCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGAAGAAAAACTCCGAATCCAAGAGCggc > 2:1015396/1‑141 (MQ=255) gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTAt < 1:520649/143‑1 (MQ=255) tagggtCATTTGCCGGCCGCAAAGAAATCATCAAACTCGGCGAGCTCCTGGAAGACCTCATGCAAACCTACAGCGGCCCGATTGGTGCCGAGTATATGCACATTACTAGCACCGAATAAAAACTCTGGATCCAACAGCGTATc < 2:815304/138‑1 (MQ=255) tttGCCAGCGGCAAAGAACCCATGAACCTCGGCGAGCTGCTGGAGGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg < 1:342475/143‑1 (MQ=255) tGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTc < 1:810692/143‑1 (MQ=255) cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg > 1:814688/1‑143 (MQ=255) gCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcgcg < 1:203356/143‑1 (MQ=255) ccATGAGACTCGGCGAGCCGCTGGAAGCCCTCAAGCAAAACTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc < 1:983939/143‑1 (MQ=255) aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga > 1:224035/1‑143 (MQ=255) tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACCTTCAGTACCGAAGAGaag > 1:952533/1‑142 (MQ=255) cGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACCGCGGCCCGACTGGTGCCGCGCACATGCACATTACCCGCACCGAATAAAAACTCTGGATCCAACAACGAATTGATCATGGACGAGCGACTTTCAATAGCGAAGCGaaaa > 1:1031372/1‑143 (MQ=255) aGCTGCTGTAAGCGCCCAAGCAAACCTACTGCCGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCCAAGAACAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc < 2:44882/143‑1 (MQ=255) ctgctgGAAGCCCTCAAGCAAACCTACTGCTGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt < 1:628987/143‑1 (MQ=255) gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttct < 1:967105/143‑1 (MQ=255) ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt > 2:1008288/1‑143 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATTCAACACCGGATCCAGTCTGGTCGCGCGACTTTCAATAGCGACGAGCAAAAACGCTTGTTAAg > 2:644091/1‑143 (MQ=255) aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 2:439341/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGGAAAACAATGGAGACAAAAGAGCAGAAATTCCTTTCGCGCGACATTCCATAGCGAAGAAAAAAAACGCTTCTTAAGCGGACt > 2:375488/1‑143 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGCGAAAAAACGCTTCTTAAGCGAACt > 1:425264/1‑143 (MQ=255) aagcaagcCTCCTGCGGCCGGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAACACCTCTGGATCCAACCGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:156818/143‑1 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCCATAGCGCAGAGAAACACCGCTTCTTAAGCGAACTGAccgccg > 2:702800/1‑143 (MQ=255) aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 1:795849/143‑1 (MQ=255) aCTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 1:130952/143‑1 (MQ=255) gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 2:799284/143‑1 (MQ=255) gATTGGTGGCGAGTATAGGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc < 1:759757/143‑1 (MQ=255) ggcgCCGAGTATATGCACATTACCAGCACCGAAGAAGAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 1:1038932/140‑1 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAACGCCGCTGAAGGTCTTGAACCTTACCTc > 2:315821/1‑143 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGCCGCGCGCCTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGGCCTGACCGCCGCTGAAGGTCCCGAACGTTACCTc > 2:292969/1‑143 (MQ=255) tGCACATTTCTCGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTACGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa < 2:952533/143‑1 (MQ=255) ttACCAGTACCGAAGAATATCCTTGTATCCAACTGCTTATCTAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACTCTTCTTAAGCGAACTGACCGCCTCTGAAGGTTTTGAACGTTACCTCGGCGCAAAATTCCCt < 2:2466/143‑1 (MQ=255) aagaaAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGCAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCCCTGAAGGTCCTGAACGTTACCTCGGCGTAAAGTTCCCTGGCGCACAACGc > 2:908477/1‑143 (MQ=255) gaaACACTCTGTATCCCACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATCGCGAAGCGAAAAAACGCTTCTTAATCGAACTGACCGCCGCTGAAGGGCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt < 1:235863/143‑1 (MQ=255) aaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGGCGCAAAACGCTTCTCg > 1:495/1‑143 (MQ=255) | CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759119‑759389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |