Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,520,500	Δ1 bp	13.1%	intergenic (‑176/+171)	yjhU ← / ← yjhF	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,500	0	A	.	13.1%	165.6 / 22.5	61	intergenic (‑176/+171)	yjhU/yjhF	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU/KpLE2 phage‑like element; putative transporter YjhF
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base . (4/4);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-02

CATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGA  >  NC_000913/4520358‑4520639                                                                                                                                               |                                                                                                                                             cATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTCTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGgaga                                                                                                                                              >  1:863595/1‑143 (MQ=255)  aTCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                                                             >  2:26540/1‑143 (MQ=255)       aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑Ct                                                                                                                                       >  2:699624/1‑143 (MQ=255)           cTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCgg                                                                                                                                   >  2:748728/1‑143 (MQ=255)            ggTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCACTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                                                                  <  2:317880/142‑1 (MQ=255)              tttACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                                                                >  1:770756/1‑143 (MQ=255)              tttACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                                                                <  2:240047/143‑1 (MQ=255)              tgtaaccGGCGAGTGGATGCAGATGGGCAGTGGCATGATTTTATACAGAGATTTTATTCTTTTACATGAAATAAACCAGTATACTATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                                                                <  1:1012789/138‑1 (MQ=255)                  cctggAGAGTTGATGCAGTTTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                                                            <  1:340474/140‑1 (MQ=39)                  cccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                                                            <  1:377990/143‑1 (MQ=255)                    cGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGt                                                                                                                          >  2:317510/1‑143 (MQ=255)                     ggAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAGA‑CTCGGGCATTACAGTa                                                                                                                         >  2:57757/1‑143 (MQ=255)                            tGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTga                                                                                                                  <  1:321333/143‑1 (MQ=255)                               tGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                                                               <  2:724789/143‑1 (MQ=255)                               tGCACATTGCCAGTCGCATGATGTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                                                               <  1:246404/143‑1 (MQ=255)                                          aGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCACCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                                                                    >  1:558865/1‑143 (MQ=255)                                           gTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAGATAAACCAGCATACCAGTGGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                   <  2:110163/143‑1 (MQ=255)                                             ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGGTCTCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                                                                 <  2:834343/143‑1 (MQ=255)                                                aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTCTACAGTATTACTGAGAATACACCCATCAAGCCTa                                                                                              >  2:328178/1‑143 (MQ=255)                                                  gATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAt                                                                                            >  1:873372/1‑143 (MQ=255)                                                     tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGa                                                                                         <  1:488984/143‑1 (MQ=255)                                                      ttATACAGATATTTCAGTCTTTTACGTGAAATAAACCAGCATACCATTTGCGCGATCGCCCGCTGTTCCCCCAGACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                                                        <  2:589981/143‑1 (MQ=255)                                                              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGATGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                                                                >  2:635872/1‑143 (MQ=255)                                                                   ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCTACTGTAAAACCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCCTCAACCATAATCGACGGGGCAAGATCGc                                                                           >  2:799567/1‑143 (MQ=255)                                                                    tATTGTTTTACATGAAATAAACCAGCATACCATGTGAGCGATCGCCCGCTGGTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                                                          <  1:455038/143‑1 (MQ=255)                                                                       tCTTTTACGTGAAATAATCCAGCATACCATTTGAGCGATCGCCCGCAGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCt                                                                       <  2:191200/143‑1 (MQ=255)                                                                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCACGCATCATCGACGGGTCACTATCGccccgt                                                                      >  1:1055289/1‑141 (MQ=255)                                                                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCTCATACTGTAAAGCCGGAGACATG‑CTCCGGCTCTACAGTATTACTGCGAAGACACCCATCAAGCCTAATCGAAGGTTCAATATCGcgccgtc                                                                     >  2:582809/1‑140 (MQ=255)                                                                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCGTAATCGAACGTTCAATATCGCCCCttt                                                                     >  1:125033/1‑143 (MQ=255)                                                                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttt                                                                     >  1:957995/1‑143 (MQ=255)                                                                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACACATCAAGCATAATCGAAGTTTCAATATCGCCCCttt                                                                     >  1:654359/1‑143 (MQ=255)                                                                          tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                                                    <  1:699624/143‑1 (MQ=255)                                                                            tACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                                                                  <  1:317510/143‑1 (MQ=255)                                                                             aCATGTAATAAACCAGCATCCCATTTGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                                                                 <  2:42959/143‑1 (MQ=255)                                                                              caggAAATAAACCAGCATACCATTTGCGCGATCGACCGCAGAGCACCCAGACTGTAAAGCCTGAGACATG‑CTCCGGCTTTACAGTATTACTGAGTATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                                                                <  2:780424/140‑1 (MQ=255)                                                                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                                                                <  1:173043/143‑1 (MQ=255)                                                                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                                                               <  1:424053/143‑1 (MQ=255)                                                                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATAACTTGCCTTTGTGATc                                                               >  1:684661/1‑143 (MQ=255)                                                                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTACCCCATACTGTAAAACCGGAGACATG‑CTCCGGCTTTACAGTATCACTCAGAATACACCCAGCAAGCATAATCGACGGTTCAATATAAACCCTTTTTGATc                                                               >  2:590245/1‑143 (MQ=255)                                                                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATA‑CTCCGGATTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTGAATATAGCCCCTATATGATCg                                                              >  1:385639/1‑143 (MQ=255)                                                                                 gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGc                                                             <  1:475860/143‑1 (MQ=255)                                                                                        aCCAGCATACCGTTGGAGCGATCGCCCGCTGTTCCCCCCTCCTGTAAACCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                                                      <  2:382316/143‑1 (MQ=255)                                                                                        aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTCATCGCCAGCggt                                                      >  2:394693/1‑141 (MQ=255)                                                                                          cAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                                                    >  1:814544/1‑143 (MQ=255)                                                                                                     ttGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTAAATGCTTTATTTAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa                                         <  1:807411/143‑1 (MQ=255)                                                                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg                                        >  1:372843/1‑143 (MQ=255)                                                                                                               gCCCGCTGTTCCCCCATTCTGTATAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCTGCGGTTCGTCACCGTGCAGACGGTTGcc                               <  2:136073/143‑1 (MQ=255)                                                                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGATCAATATCGCCCCTTTTTGCTCGCCAGCGATTCGTCACCGTGCAGACGGTTAcc                               >  2:2797/1‑143 (MQ=255)                                                                                                                cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCGGACGGTTGGCg                              >  2:284847/1‑143 (MQ=255)                                                                                                                cccGCTGCACCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAGTCGTCACCGTGCAGACGGTTGCCg                              <  1:934775/143‑1 (MQ=255)                                                                                                                 ccGCTGTTCCCCCCTACACCATAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGt                             <  2:823251/143‑1 (MQ=255)                                                                                                                    cTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcac                          <  2:957995/143‑1 (MQ=255)                                                                                                                     tGTTCCCCCATACTGTAAAGCCGGAGACCTG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcact                         <  2:863595/143‑1 (MQ=255)                                                                                                                          ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCACCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTa                    >  1:228807/1‑143 (MQ=255)                                                                                                                             cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCCCCGTGCAGCCGGTTGCCGTCACTCACTAAAt                 >  2:410202/1‑143 (MQ=255)                                                                                                                                aCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAACTTAt              >  2:558556/1‑143 (MQ=255)                                                                                                                                 gtGTAACGCCAGAGACATG‑CTCCGGCTTTACAGTATTACTGAGACTACACCCATCGAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg             <  1:987514/142‑1 (MQ=255)                                                                                                                                  tGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg            <  2:873372/143‑1 (MQ=255)                                                                                                                                    tAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgggg          >  1:1018935/1‑143 (MQ=255)                                                                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCACTATGGGCCCTTTTTGATACCCAGCCAGGCGTCACCGTGCAGACGGTTGCCGTGACTCACTAAATTATggggt         >  1:207392/1‑142 (MQ=255)                                                                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATCTCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa        >  1:304375/1‑143 (MQ=255)                                                                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCGCTCACTAAATTATGGGGGa        >  1:480435/1‑143 (MQ=255)                                                                                                                                            tgtaCATG‑TTCCGGCTGGACCGTCTGACAGAGAATACACCCATCCAGCATCCTCGAAGTTTCATTATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa  <  2:1065828/140‑1 (MQ=255)                                                                                                                                               |                                                                                                                                             CATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGA  >  NC_000913/4520358‑4520639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑