| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 419,670 | Δ1 bp | 81.1% | coding (80/1320 nt) | brnQ → | branched chain amino acid transporter BrnQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 419,670 | 0 | T | . | 81.1% | 90.9 / 24.5 | 37 | coding (80/1320 nt) | brnQ | branched chain amino acid transporter BrnQ |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base . (17/13); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01 | |||||||||||
CCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAG > NC_000913/419533‑419811 | ccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCCTCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TAtttt > 1:421748/1‑143 (MQ=255) cAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTCTGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTCCGTTCTTCGTCGGCGCAGGTAACA‑TATTTTc > 1:592076/1‑143 (MQ=255) tgcgATGGGCACAAAAATTCAACGTTACATCCCCACATCCACGGGCAGTATGATTTATGACCCATCAATGAAGATCGCGCGATATCATCGCTCTGGGCTTTATGTCATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTcc < 1:519378/140‑1 (MQ=255) ggATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCt < 2:197782/143‑1 (MQ=255) ttGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGAGCAGGTAACA‑TATTTTCCCTCCa < 2:969758/143‑1 (MQ=255) caAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGt < 2:771397/143‑1 (MQ=255) caAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTc < 2:288023/143‑1 (MQ=255) aaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCCATGGTCGGc > 1:494162/1‑143 (MQ=255) aTTTAACGTTACAACACCACATCCCCAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATTACATTTGCGTTGTTCATCGGCCCAGGTAACA‑TATTTTCCCTCAGATCGTCAGC‑tt > 2:790207/1‑143 (MQ=255) ttAGCGTTACAACTTGACATCCACAGGCAGTATGATTTAGGACCCATCAATTAAGCTCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTAc < 2:890820/143‑1 (MQ=255) cGTTACAACACCAAATCCGCAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGC‑TTAcagg < 2:43378/143‑1 (MQ=255) gTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTAcaggca < 2:130986/143‑1 (MQ=255) tACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTAcaggcagg > 1:458628/1‑143 (MQ=255) tACAACACCACATCCACAGGCAGTATGATATATGACCCATCAATTAAGATCAAAAGTGAGCATCGCCCTGTTCATTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTTAcaggca > 2:1077940/1‑143 (MQ=255) caccacATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTAcaggcag < 1:695602/137‑1 (MQ=255) caccacATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTAcaggcag > 2:695602/1‑137 (MQ=255) caccacATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGCac > 2:987289/1‑143 (MQ=255) ccacATGCACAGGCAGTATGATTTATGCCACATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAacac < 2:592076/143‑1 (MQ=255) cacaTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAAGA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGCACACg > 1:278410/1‑143 (MQ=255) caTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCCGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCACGCGAACCCGTc > 2:319996/1‑143 (MQ=255) caTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGACTTTATGACAGTTGCGTTGTTCGGCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTc > 1:697180/1‑143 (MQ=255) tCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTg < 1:679037/143‑1 (MQ=255) cATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGGTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGAGCCGC‑GTACAGGCAGGCGACCACGTCTGGACTCCGGCATGCGGCATcgtgac > 2:830650/1‑138 (MQ=255) aaTTAAGATCGTGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTACTCTCCCCTGGTCCGC‑TCGGTGGCGGGCGCACACGTCTGGACTGCCGCCTCCGCCTTCCTCATTACTg > 2:783952/1‑143 (MQ=255) aTTAAGATCGCGCGATATCATCGCTCTGGGCTTGATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCGCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGc < 1:971583/143‑1 (MQ=255) tAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGTAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTCCCg > 1:142641/1‑143 (MQ=255) aTCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTgg > 2:114904/1‑143 (MQ=255) tCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGGTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCCTTCGCCTCCCTCATTACTGCCGTTGGc > 2:873628/1‑143 (MQ=255) gcgcgATATCATCGCTCTGGGCCTTATGACATTCGCGTTGTTCGTCGGCGCAGGTAACA‑TATCTTCCCTCCAATGGCCGGC‑TTACAGGCCGGCGAACACTCCTGGACTGCGTCATTCGGCTCGCTCGTCACCTCCGTCGGcca > 2:268871/1‑142 (MQ=255) tctGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCt < 2:171504/141‑1 (MQ=255) tGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCt < 1:1077940/143‑1 (MQ=255) tgttCGTCGGCGCAGGTAACA‑TATCTTCCCTCCAACGGTCGGC‑TTACAGGCCGGCGAACACGTCTGGACTGCGGCATCCGCATTCCTCCTTACTGCCGTTGGCCTGCCGGCATTAACGGCAGTGGCGCTGGCAAAAGTTggcg < 1:496609/143‑1 (MQ=255) tttgtcgtcTCAGGTATCATCACTTTCGCTCCAAGTGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGTTGGCAAAAGTTggcgg < 2:462331/140‑1 (MQ=255) cgtctGCGCAGGTAACATTATTTGCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGgcggcg < 2:740373/143‑1 (MQ=255) cgtcgGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGcggcgg < 2:697180/143‑1 (MQ=255) cgcAGGTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCACAGGTTGGCGGCGGTGTTGa > 2:33012/1‑143 (MQ=255) gTAACA‑TATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGAGGCATTCGGCTGCCTCATTACTGCCGTTGGCCTGCCGGTGTTAGCGCTAGGGGAGCTGCGAAGAGTTGGCGGGGGTGTTGACAGcc > 2:33454/1‑143 (MQ=255) tagcttattGTCCCTCCAGTGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCAGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCt < 2:878987/139‑1 (MQ=255) attattTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAGGTTGGCGGCGGTGTTGACAGCCTCAg > 1:912047/1‑143 (MQ=255) ttattTTCCCTCCAATGGTCGGCTTTACAGGCAGGCGAACACGTCTGGACCGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCCAAAGGTGGCGGCGGTGTGGACAGCCCCAg > 2:257599/1‑143 (MQ=255) | CCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGC‑TTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAG > NC_000913/419533‑419811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |