| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 786,458 | Δ1 bp | 100% | coding (826/1053 nt) | aroG → | 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 786,458 | 0 | A | . | 97.8% | 190.5 / ‑3.2 | 46 | coding (826/1053 nt) | aroG | 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (27/18); minor base C (1/0); total (28/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCATATCATTCTGCGCGGCGGTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTA > NC_000913/786318‑786596 | gCCATATCATTCTGCGCGGCGGTAAAGAGCCTACACGA‑AACGGGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTCaaa < 2:230323/143‑1 (MQ=255) atatCATTCGGCGCGGCGGTAAAGAGCCTC‑ACCGCAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTCaaaagc < 1:298629/143‑3 (MQ=255) cATTCTGCGCGGCGGTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAGTTC‑AAAAGCAgat > 1:40487/1‑143 (MQ=255) ttCTGCGCGGCGGTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGCGTGGAGGGATGTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AGAAGCAgatgg > 1:357280/1‑143 (MQ=255) gcggcgGTAAAGAGCCTA‑ACTACAGCGCGAAGCACGATGCTGAAGTGACAGAAGGGCTGAACAAAGCAGACCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGAAGGAGGtta > 2:214861/1‑142 (MQ=255) ggcggTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTtgt > 1:360124/1‑143 (MQ=255) gcggTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTtgtg < 2:1011206/143‑1 (MQ=255) gCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTg < 1:33246/143‑1 (MQ=255) tA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCca > 1:441563/1‑143 (MQ=255) aCTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCcagc < 1:237345/143‑1 (MQ=255) tACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCcagcag > 1:132336/1‑143 (MQ=255) cgcgAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCAATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGAAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGc > 1:308285/1‑143 (MQ=255) cgcgAAGCACGTTGCAGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTACTGACGTTTGCCAGCAGATTGc > 2:931824/1‑143 (MQ=255) cgCTGAAGTGCGAGAAGGGCTGAACAAAGCACCCCTGCCAGCACCGCTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGaaaa < 2:880612/142‑1 (MQ=255) gCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAagag > 2:55329/1‑143 (MQ=255) gCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAg < 1:1086192/143‑1 (MQ=255) aaaGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGATGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACCATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTACCGGTGGCGAAAAGGCCactatt > 1:716208/1‑143 (MQ=255) aaaGAAGGGCTGAACAAAGCAGGACTGCCAGCACAGGTGATGAGCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGAGGGGAGTTACTGATGACGTTTGCACGCAGATTGCCGGTGGGGAAAAGGCCattatg > 2:560200/1‑142 (MQ=255) agGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGt < 1:951584/143‑1 (MQ=255) agGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGt < 2:441563/143‑1 (MQ=255) cTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCAAGATAACCCATCCATACCATTTGCATTATCACCCGG‑‑GTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAtg > 1:810984/1‑143 (MQ=255) aGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAg > 2:93757/1‑143 (MQ=255) ccggCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAGAGCAGATGGATGTGTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGcc < 2:811264/141‑1 (MQ=255) gCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGACGTTCGTGCTGCAGATCGCCAGCAGATCGCCGGTGGCGAAAAGGCCACTATTGCTCTGATGGTGGAATGCCaac > 2:279403/1‑141 (MQ=255) cTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCAGTGTTGGCGTGATGGTGGAAAGCCATCtg > 1:122568/1‑143 (MQ=255) cTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGATTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATcgg > 2:45426/1‑141 (MQ=255) gcaaGCACAGGTGATGACCGATCTCCGCCACGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATCGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCtggt < 1:559787/140‑1 (MQ=255) gCCAGCACAGGTGATGACCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCtggt < 1:32145/143‑1 (MQ=255) gCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCCTTATTGGCGTGATGGGGGAAAGCCATCGGGTGGaa > 2:69128/1‑143 (MQ=255) gTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCaaa > 1:1107721/1‑142 (MQ=255) gTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATAGTAGAAAGCCAGCTGGTGGAAGGCAAt > 2:334967/1‑143 (MQ=255) tgatgaTCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATc < 1:1101429/143‑1 (MQ=255) tgatCGATTACACCCATGCTAACTCGTCCA‑ACCAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGCTTGCCGGTGGCGAAAAGGCCCTTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCaga < 1:396791/143‑1 (MQ=255) atCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCCGCAGATTGCCGGTGGCGAAAAGGCCATTATAGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGc > 1:56769/1‑143 (MQ=255) atCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGc > 2:863665/1‑143 (MQ=255) atCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAAATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGc > 1:33623/1‑143 (MQ=255) tCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGcc > 2:253724/1‑143 (MQ=255) gATTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AATAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTc < 2:865479/143‑1 (MQ=255) aTTTCAGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGCTGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAAGCAGGGCcgcg > 2:590899/1‑143 (MQ=255) aGCCATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGGGGAAGGCAATCAGAGCCTCGAGAGc > 2:114769/1‑143 (MQ=255) ccATGCTATCTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCgg < 2:752057/143‑1 (MQ=255) ccATGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCgg > 1:1027166/1‑143 (MQ=255) tGCTAACTCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCCGCCGTTGGCCAGCAGATTGCCGGTCGCGAAAAGGCCATTATTTGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGa < 1:95206/143‑1 (MQ=255) ggCTAACTCGATCA‑AACCGTTC‑AAACGCAGATGGATGTTTGTGCTGACGTTTGCCAGGAGCTTGCCGGTGGCGAAAAGGCTATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGa < 2:158839/142‑1 (MQ=255) tCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAtggtg > 1:184550/1‑92 (MQ=255) tCGTCCA‑AACAATTC‑AAAAGCAGATGGATGTCTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAtggtg < 2:184550/92‑1 (MQ=255) ggccA‑AACAATTC‑GAAGGCAGATGGATGTTTGTGCGGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGc < 2:467679/141‑1 (MQ=255) aaTTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTCTTGGCGTGATGGTGGAAAGCCATCTGGTGGCAGGCAATCAGCGCCTCGAGAGCGGGGCGCCGCCGGCCTACGGTa > 2:27514/1‑143 (MQ=255) | GCCATATCATTCTGCGCGGCGGTAAAGAGCCTA‑ACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCA‑AACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTA > NC_000913/786318‑786596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |