Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,330,402	Δ1 bp	6.5%	intergenic (+21/+15)	sohB → / ← yciN	S49 peptidase family protein/protein YciN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,330,402	0	A	.	6.5%	154.0 / 7.2	62	intergenic (+21/+15)	sohB/yciN	S49 peptidase family protein/protein YciN
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base . (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02

ACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGC  >  NC_000913/1330270‑1330543                                                                                                                                      |                                                                                                                                              atgCTTTATTCATCGTTATCGTCTCATTGACCGATTAGACGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt                                                                                                                                     <  1:115396/141‑1 (MQ=255) aCGCTTGATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt                                                                                                                                     <  2:1044730/143‑1 (MQ=255)    cTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGAGTGTTGCTACGCTGGTGGCAGCGGGGTCCAAAGCCATTGACGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGc                                                                                                                                  >  2:182862/1‑143 (MQ=255)    cTAGATGCAGGGTAAACGGCGCGTTGACCGATTCACCGGCAGAGGGAATCCGCAGTCCTATCGGTTGTGGCGCCGCGGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGc                                                                                                                                  <  2:492412/143‑1 (MQ=255)      atatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCtt                                                                                                                                <  1:766135/143‑1 (MQ=255)      atatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTATTGAtt                                                                                                                                >  1:848634/1‑143 (MQ=255)        atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTa                                                                                                                              <  1:183172/143‑1 (MQ=255)          gCAGCGTGCACGACTCCTTGACCGCTTCGTCGGCAGCGCGGCACAGCGCGCCGATCCATTGTTGGTCCGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTACACGACCAACGCGCCG‑CGGAGACCTCGCGTTTTGCTTTAAt                                                                                                                            <  2:966639/143‑1 (MQ=255)              cGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                        >  1:787126/1‑143 (MQ=255)                 aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATAGATGTAAAAGACAAACGCCAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACAAg                                                                                                                     >  2:689750/1‑143 (MQ=255)                  aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAga                                                                                                                    <  1:110539/143‑1 (MQ=255)                  aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCCCGCGTTTTGCTTTAATCAACCAga                                                                                                                    <  1:353813/143‑1 (MQ=255)                   aCGTCTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat                                                                                                                   <  2:354896/143‑1 (MQ=255)                       cTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata                                                                                                               <  2:1054206/143‑1 (MQ=255)                                   ttCACCGGCAGCTCTGTATATATCATCAATCGATTTTTGCTACGCTTGTTGCATCGGTGTCAAAATCCATTTATGTAAAATACAAACTCGAGT‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATTATATTTTTCTTAAAg                                                                                                   <  2:894529/143‑1 (MQ=255)                                   ttCACCGGCAGCTCTGCACAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCATCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGATG‑CTAAGACCTCTCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg                                                                                                   <  2:510286/143‑1 (MQ=255)                                    tCACCGGCAGCGCGGCACAGATCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGTTCAAAATCCAGTTATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGc                                                                                                  <  2:835285/143‑1 (MQ=255)                                      aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAACGCCATTGCTGTAGAAGACAAACGCGAGG‑CTAAGCCATCGCGTTTTGCTTTAATCAACCAGCTGATATTTTTCTGCAAGcac                                                                                                >  2:936829/1‑143 (MQ=255)                                      aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGCTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac                                                                                                <  1:988194/143‑1 (MQ=255)                                                      agagCGCGGATCGATTGTTGCTAAGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGGGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTg                                                                                <  2:690554/143‑1 (MQ=255)                                                           gcCGCTCGATTCTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAACACCTCGCGCTTTGCTTCAATCAACCAGATGACATCTTCCTGAAAGCACATCAGCCACGCGCTTGagcct                                                                           >  2:403393/1‑139 (MQ=255)                                                             cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACatat                                                                         >  1:254858/1‑143 (MQ=255)                                                               atcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACAGATTa                                                                       >  1:368116/1‑143 (MQ=255)                                                                  gatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCCGGTGTTTGAACATATTAAac                                                                    >  1:976097/1‑143 (MQ=255)                                                                    ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGCACATATTAAacac                                                                  >  2:955993/1‑143 (MQ=255)                                                                    ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTCAacac                                                                  >  1:850131/1‑143 (MQ=255)                                                                        tgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAGACACcgcg                                                              >  1:837024/1‑143 (MQ=255)                                                                           tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTg                                                           >  1:810252/1‑143 (MQ=255)                                                                              gCTGGTGGCAGCGGGGTCAAAAGCCATTGGCATAAAAGACAAACGCGAGG‑AAACGACATAATTATTAGATTTTATCAAGAAGATGATATTACTCTGAAAGCACATGGGCCAGGTGTTGGAACGTATTAAACATCGCGGtaatg                                                        >  2:785610/1‑139 (MQ=255)                                                                                 ggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACACGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTCTTg                                                     <  2:966675/143‑1 (MQ=255)                                                                                  gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCATGTGTTTGAACATATTAAACACCGCGGTGCTTTTgg                                                    <  1:21041/143‑1 (MQ=255)                                                                                       aGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGAGGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGtt                                               <  1:837934/143‑1 (MQ=255)                                                                                         cGGGGTCAATAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGGTTTGCTTTAATCAACCAGATGATATTTTTCGGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                             <  2:681472/143‑1 (MQ=255)                                                                                            ggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCaa                                          <  1:666039/143‑1 (MQ=255)                                                                                             gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTCGCACCTATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                         >  2:403377/1‑143 (MQ=255)                                                                                                aaaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCACTcac                                      >  2:472698/1‑141 (MQ=255)                                                                                                 aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGCTATTTTTCTGAGAGTACCTGTGCCAGGTGTTTGACTATATTCAGCACTGCGGTGCTTTTTTCTGTTGTCAAtacct                                     >  2:126996/1‑139 (MQ=255)                                                                                                   aGCCATTGATGTAAAAGACCAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                                   <  1:301496/143‑1 (MQ=255)                                                                                                     ccATTGATGTAAATCACAAACGCAAGG‑CTAATCCCTCGCGTTTTGCTTTAATCATCCAGATGATATTTTTCTGAAAGCTCATGGGCCAGGTGTTTGAACATATTAAACGCCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                 <  1:954742/143‑1 (MQ=255)                                                                                                            tgaaaaaGACGAACGCGCGG‑CTAAGACCTCGCGGTTTGCTTTAATCACCCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaa                          <  1:80749/140‑1 (MQ=255)                                                                                                              tacaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                        <  1:726206/140‑1 (MQ=255)                                                                                                              tAAACGACAAACGCGCGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                        <  1:797222/143‑1 (MQ=255)                                                                                                              tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                        <  2:787126/143‑1 (MQ=255)                                                                                                              tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                        >  1:892498/1‑143 (MQ=255)                                                                                                              tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAATTTTCAGAAAGCACAAGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                        >  1:1047935/1‑143 (MQ=255)                                                                                                                aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt                      >  1:552838/1‑143 (MQ=255)                                                                                                                aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt                      >  1:25105/1‑143 (MQ=255)                                                                                                                 aggACGAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATCTTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                     <  2:537011/141‑1 (MQ=255)                                                                                                                 aaGACAAACGCGGGG‑CTAAGACCTCGGGTGTTGCTTTAATCAACCAGATGATACTTTTCTGAAAGCCCGTGGGCCAGGTGTTTGATCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                     <  2:301764/143‑1 (MQ=255)                                                                                                                  aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTATTCATCTAAAAagtac                    >  2:688854/1‑142 (MQ=255)                                                                                                                  aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCCGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                    >  2:689041/1‑143 (MQ=255)                                                                                                                     aaaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                 >  1:1013871/2‑143 (MQ=255)                                                                                                                      aaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                >  1:540206/1‑143 (MQ=255)                                                                                                                      aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                >  1:328457/1‑143 (MQ=255)                                                                                                                      aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                >  1:728812/1‑143 (MQ=255)                                                                                                                      aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                >  1:818461/1‑143 (MQ=255)                                                                                                                        aCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg              <  1:112394/143‑1 (MQ=255)                                                                                                                         ctcgACG‑CTAAGACCTCGCGTTTTGCGTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTTCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg             <  1:314472/141‑1 (MQ=255)                                                                                                                              gg‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGGACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTGAAAAGTAGTCGCCGGTGAAt        >  1:127413/1‑143 (MQ=255)                                                                                                                                 cTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAc      <  1:675210/143‑1 (MQ=255)                                                                                                                                 cTAAGACCTCGCGTGTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAc      <  2:406690/143‑1 (MQ=255)                                                                                                                                     gACCTCGCGTGTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGc  <  1:106877/143‑1 (MQ=255)                                                                                                                                      |                                                                                                                                              ACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGC  >  NC_000913/1330270‑1330543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑