| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 89.2% | 92.6 / 5.3 | 37 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (13/19); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATA > NC_000913/3637503‑3637774 | gAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTCACGTCCTATG‑c > 1:597371/1‑143 (MQ=255) aaGATAATTATTTTTTGAGTAAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTATGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑Atttg < 1:263769/143‑1 (MQ=255) attattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCt < 2:304810/143‑1 (MQ=255) ttattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTg < 1:365457/143‑1 (MQ=255) tttttGAGTGAAATCCAGACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCt < 2:426337/143‑1 (MQ=255) ttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTCTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGCCCTCAAAATGGCGTAACGTCCTATGCC‑T‑AC‑ACTTGTTCGCTGGCCTg > 2:85011/1‑143 (MQ=255) ttGAGTAAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGa < 2:232468/143‑1 (MQ=255) aGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATGTGTTTGCTGGCCTGGAttt < 1:526199/143‑1 (MQ=255) atgcggggggCGACTCAAACAAGGTCTCTATTTCGCTTTGTCCGCGCCGCGAAATGCACTGATAATGCGCCGCGTTCATGTCCGCAAAATGGCGTAGAGTCGTGTC‑C‑A‑ACAATATATGGGCGGGCCTGGCTTTTCATACCggg < 2:1084532/138‑1 (MQ=255) tACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATACTGCGCCGCGTTCATGTCCTCAAAATGGCGCAACGTCCTATG‑CTT‑AC‑ATTTGCTTGCTGGCCTGCATTTGCATACGGGGc > 2:700727/1‑143 (MQ=255) tACAGGGGGCAAATCAAAAAAAGTCTATAGTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGc < 1:26820/143‑1 (MQ=255) aGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCGATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGt > 2:326171/1‑143 (MQ=255) gCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta > 1:765642/1‑143 (MQ=255) aaaTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTattatt < 2:375217/143‑1 (MQ=255) tCAAAAAACGTCTATATTTCACTTTGCCCGCGCCGCGCAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTTCTGACCCGGATTCACCTACCGCACTGTTAGTAttgct > 2:760686/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg > 2:682606/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCAGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGTCTTtgtg > 2:472978/1‑143 (MQ=255) cacgtggcccgcccCGCGAAAGTCGCTTGTAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTg < 2:452635/137‑1 (MQ=255) cACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTg < 1:734658/143‑1 (MQ=255) tGCCCGCGCCGCGAACGTCACGGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTGTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTa < 2:1005851/143‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTGCTGCGaa > 2:573438/1‑143 (MQ=255) ccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCGATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCa < 1:326171/143‑1 (MQ=255) caaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCaa < 1:502636/142‑1 (MQ=255) aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTc > 1:710590/1‑143 (MQ=255) aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGCGCTGGTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTc > 2:956904/1‑143 (MQ=255) cTGATAATGCGCCTCCTTCATGTCCTAAAAATTGCTCAACGTCCTATGTT‑T‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTcc < 2:744800/143‑1 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 1:67244/143‑1 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 2:573487/143‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCCATC‑CGC‑AC‑ATTGGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 2:597212/143‑1 (MQ=255) gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTTTTATTTCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGc < 1:466426/143‑1 (MQ=255) gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCCATGGTTTCCATGACACAGc > 1:3215/1‑143 (MQ=255) gTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTgg < 1:584092/143‑1 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa > 1:963960/1‑143 (MQ=255) tCAAAATGGCGTAACGACCTACG‑C‑TGAC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCCTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAAc < 1:245723/143‑1 (MQ=255) aaTGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCCTGACTGGTTTCCATGCCACAGCCAACGCCGTGGCCACCGtt > 2:286275/1‑143 (MQ=255) aaTGGCGTAACATCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCACTGGTTTCCATGACACGGCCAACGTCGTGGCGACCGtt > 1:844575/1‑143 (MQ=255) tGGCGTAACGTCCTATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCACCCGGGGGATGAATCATGGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 1:1097869/143‑1 (MQ=255) tAACGTCATATG‑CTT‑AC‑ATTTGTTTGCTGGCCTGGATTTGCCTACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCtata < 1:536299/143‑1 (MQ=255) | GAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑AC‑ATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATA > NC_000913/3637503‑3637774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |