| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,868,865:1 | +G | 6.5% | intergenic (+23/‑90) | yeaG → / → yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,868,865 | 1 | . | G | 6.5% | 239.1 / 11.4 | 62 | intergenic (+23/‑90) | yeaG/yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Reads supporting (aligned to +/- strand): ref base . (31/27); new base G (2/2); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01 | |||||||||||
AGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGT > NC_000913/1868733‑1869004 | acaagaaaaAACACAACGACTTTGCCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGcca < 1:482455/140‑2 (MQ=255) cAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTAc > 2:720769/1‑143 (MQ=255) cAGAAGAAACACGACGACTTTGTCGACCGTAAGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTAGTTGCGCGTACGGAAATCGTCTTAACAACCCTGGCCCGGTACACATGG‑TACCGGGCCGAc > 1:816567/1‑143 (MQ=255) aagaaACACGACGACTTTGTCGACCGTCTGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTAGTTGCGCGTCCGTAAATCGTCTTGACAACCCATGCCCGGCACACATGC‑TACGGCGACTAccag > 2:349445/1‑140 (MQ=255) aaaCACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGAc > 1:678068/1‑143 (MQ=255) gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGaa > 1:614901/1‑143 (MQ=255) cTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTg > 1:64243/1‑143 (MQ=255) ggggcGACCGTAGGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTGACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTggg < 1:1083579/139‑1 (MQ=255) tCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCt < 1:842373/143‑1 (MQ=255) cGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGGACACATGT‑TACCGGGCCTACAACGACAGCGACCCGAGGCCCTg > 2:1035570/1‑143 (MQ=255) ggatgatgGAAAAAGGCTACAGCCGGAAACAGGTGCGGTTACTGTGCGAATGGTATTTGCGGGGACGTAGATCGTCGCAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg < 1:930051/141‑1 (MQ=255) gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGTCCCGGTACACATGT‑TACCGAAACTACAACGACAACTAACCGTGTGCCTGAGaaa > 1:764922/1‑142 (MQ=255) gTATGATGGAAAAAGGCTACACCCGTAAACAGGGGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg < 1:391195/143‑1 (MQ=255) tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTCAATCGTCTTAACAACCCTGGCCCGGTAAACATGA‑AAAAAGGACAAAAACGACAGGGAACCGTGGGCCTGAGAAga > 1:620234/1‑142 (MQ=255) tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg < 1:109475/143‑1 (MQ=255) atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGCAGCGGCa > 1:212229/1‑143 (MQ=255) atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACAAATGT‑TACGGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAGGCGGCa > 1:927657/1‑143 (MQ=255) tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 1:694638/143‑1 (MQ=255) tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 1:290074/143‑1 (MQ=255) tgGAAAAAGCCTACACCCGTAAACGGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 1:82385/143‑1 (MQ=255) aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac > 1:169477/1‑143 (MQ=255) cTACGCCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTGGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa < 1:184533/143‑1 (MQ=255) acacCCGTAATCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc < 2:277618/143‑1 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACGTAGGCGTAGCATAc > 1:245912/1‑143 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGCCGGCCTGAGCCGCGGCCACACAGGACAAGCATAc > 2:1005846/1‑143 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGAGAATGGTATTTGCGCGTACGTAAATCGAATTAACAACCCTGGGCCGGTAGACATGT‑TACCGGGAGTAACAGGACAGTGAACGGTCGACATGAGAGGAGGCAACAGAGACGtgcgacac > 2:130393/1‑136 (MQ=255) aCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTCAAACGACAGCGAACAGTGGGCCTGAGAAACGGCAACACAGGCGTAACATACAGTTga > 1:673568/1‑142 (MQ=255) gTTTACTGTTCGAATGGTATATACACGTAAGTAAATCGTCTTAACAACCCTGGACAGGTACACATGT‑TACAGGGCATACAACGACAGCGAACCGCGGGCCTGAGAACGGGCACGACACGAGAAGCATACCGTAGGCaaaacaa > 2:326787/1‑139 (MQ=255) tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagta < 2:614901/143‑1 (MQ=255) tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCTAGTGCCTTCGAAGCGGCAACACAGGCGACGCATACAGTTGGCAAATGAAGTACgg > 2:663443/1‑143 (MQ=255) tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCAGGCCCGGTA‑ACATGTGTACCGGGcca < 2:283786/71‑2 (MQ=25) tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCAGGCCCGGTA‑ACATGTGTACCGGGcca > 1:283786/1‑70 (MQ=25) gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCACATGTAGTACggg > 1:364392/1‑143 (MQ=255) gtcccaaTGGTCTCTGCGCGTACGTAACTCGTCTTAACAACCCTGGCCCTGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg < 2:1001009/138‑1 (MQ=255) aTGGTATTTGCGCGTACGTTAATCGTCTTAACACCCCTGGCCCGGTACGCATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCat < 2:166008/143‑1 (MQ=255) atgcccccgtacgtaAATCGGCCTACCAACCCGGGCCCGGTACCCATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCCGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc < 2:17306/136‑1 (MQ=255) ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt < 2:678068/143‑1 (MQ=255) agcgcGTACGTAACTCGTCTTAACACCCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACTACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg < 1:503199/142‑1 (MQ=255) gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt > 2:462598/1‑143 (MQ=255) ccctccgtaAATCGTCTTTACAACCCTGGCCCAGTACACATGT‑TACCGGGCCTACAACGCCAGCGAACCGTCGGCCTGAGACGCGGCAACACAGGCGTAGCATCTCATTTACTATTGTATTACGGGGGGCATATGACCTGGtt < 2:82084/138‑1 (MQ=255) tccgtaACTCGCCTCAACAACCCTGGCCCGGTACACATGC‑TACCGGGCCTACAACGACAGCGACCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt < 2:698735/141‑1 (MQ=255) tacgtaAGTCGTTTTAACAACCCTGGGCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt < 2:709991/143‑1 (MQ=255) tacaTCGTCGTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAc < 1:421093/140‑1 (MQ=255) gaaaTCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAc > 2:636176/2‑143 (MQ=255) aaaTCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAcc < 1:720769/143‑1 (MQ=255) aaTCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGCAGCGGCAACACAGGCGTAGCATACCGTTGGCATATGTCGGACCGGGGGCCTCTGCCCCGGGTTCTTGAcgg > 2:829449/1‑141 (MQ=255) tCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGCAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACAGGGGTGGTTGACCggg > 1:383145/1‑142 (MQ=255) gTCTGAACAACCCTGGCCCGGTACACAGGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGt < 1:85803/143‑1 (MQ=255) tAACAACCCTGGCCCGGTACACATGT‑TAACAGGCCACGCACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGa > 1:1052579/1‑143 (MQ=255) aacaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGCAGCATACAGTTGGCAAATGTAGTACGGGGGGCCTACGACCCGGTTTATTGACCGGCGTCTGaa > 2:719371/1‑143 (MQ=255) aacaacCCTGGCCCGGGCCGCAGGT‑TACCGGGCCTACCACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGaa < 1:1005846/143‑1 (MQ=255) acaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGagc > 1:492159/1‑141 (MQ=255) aacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGACGCGGCAACACCGGCGTAGCATACAGTTGGCAAACGTAGTACGGGGGGCATATGACCTGGGATATTGACCGGCGTCTGAACgg > 2:323112/1‑143 (MQ=255) aacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAGCACAGGCGTAGCATACAGTTGGCAAATGTAGCACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACgg > 2:558688/1‑143 (MQ=255) ccTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaa < 1:843095/143‑1 (MQ=255) gCCCGGTACACCTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAAc < 2:990969/143‑1 (MQ=255) cccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGCGCACCTGACCTGGTGTATTGACCGGCGTCTGGACGGCAAAAACa > 1:750473/1‑143 (MQ=255) ggTACACATGT‑CACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa < 1:595764/143‑1 (MQ=255) gTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTCTTGCCCGGCGTCTGAACGGCAAAACCAAAAg > 2:513545/1‑143 (MQ=255) tACACATGT‑TACCGGGCCTACAACGACGGCGAACGGCGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGc < 1:1043684/143‑1 (MQ=255) acaTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATg > 1:359728/1‑143 (MQ=255) aTGT‑TACCGGGCCTACAACGACAGCGAAACGAAGGCGTTAGTGTCTTCAACACAGGCGTAGCATACAGTTGGCAAATGTAGGGCGGGGGGCATATGACCTGGTTTGTGGACCGGCGGCTGAACGGGAGATGCAAAAGCGTGGt > 1:520316/1‑143 (MQ=255) | AGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGT > NC_000913/1868733‑1869004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |