Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,473,614 C→T 100% W156* (TGG→TGA)  rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,473,6140CT100.0% 152.1 / ‑6.7 50W156* (TGG→TGA) rpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (20/28);  minor base G (0/1);  total (20/30)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTT  >  NC_000913/3473474‑3473753
                                                                                                                                            |                                                                                                                                           
ccGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCa                                                                                                                                           >  2:272210/1‑143 (MQ=255)
  gATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATGTGTTTACTCTTGTAGCTTTTAGGCGTTCAATTGAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAAc                                                                                                                                         <  1:207170/143‑1 (MQ=255)
          ggTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATACTCCACGGTAgggt                                                                                                                                 >  2:253332/1‑143 (MQ=255)
          ggTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAgtgt                                                                                                                                 >  2:263800/1‑143 (MQ=255)
           gTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGtgtg                                                                                                                                <  1:951778/143‑1 (MQ=255)
              gCGTGTGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTGAGGAGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGa                                                                                                                             <  1:463333/143‑1 (MQ=255)
               cGTGCGATTGGGTTTTTACGAGCTATTTGTTTCCTCGTTTATCTTTTAGACGTTCAATTTAAGTAGCCGAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGaa                                                                                                                            <  1:173756/143‑1 (MQ=255)
                   cGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGcc                                                                                                                        >  2:256887/1‑143 (MQ=255)
                    gATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGAATAATCAACGGTAGTGTGCGAACGCCt                                                                                                                       >  2:558041/1‑143 (MQ=255)
                     gggggTGCTGTACGCGGCAGTTGTGTCCTCGGTTATCTTTTAGGCGTTCAATTGAAGTAGGCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCtt                                                                                                                      <  2:401772/141‑1 (MQ=255)
                     ctGGTTGTTGTACGAGCCATTTGTTTCCGCGTTGATCTTTTACGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCtt                                                                                                                      <  2:721706/142‑1 (MQ=255)
                        ggTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCttgtt                                                                                                                   >  1:278062/1‑143 (MQ=255)
                             tgtACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCtt                                                                                                              <  2:1099961/143‑1 (MQ=255)
                                aCGAGCCATTTGTTGCCGCGTTTAGCTTTTAGGCGTGCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                                                                           <  2:448857/143‑1 (MQ=255)
                                  gAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCCTGTTGGCTTCGGcc                                                                                                         >  2:747646/1‑143 (MQ=255)
                                   aGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCa                                                                                                        >  1:1021195/1‑143 (MQ=255)
                                       aTTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                                                                    >  1:641223/1‑143 (MQ=255)
                                        cctGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAATTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTTGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACgg                                                                                                   <  1:204630/141‑1 (MQ=255)
                                           gTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCAGACGCGGGCCGCTTACTGGAAGCGCCCGCCGTGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGa                                                                                                <  1:411833/143‑1 (MQ=255)
                                              tCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACg                                                                                             >  1:128873/1‑143 (MQ=255)
                                                 tCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCt                                                                                          >  1:910170/1‑143 (MQ=255)
                                                 tCGTTTATCTTTGAGGCGTGCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCt                                                                                          <  1:1089847/143‑1 (MQ=255)
                                                    tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                                                                       <  1:272210/143‑1 (MQ=255)
                                                            ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                                                               <  1:216884/143‑1 (MQ=255)
                                                               ggCGTTCAATTTAAGCAGCCCAAAGCGGGCTGCTGACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTTGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAAc                                                                            <  1:282345/143‑1 (MQ=255)
                                                                   ttCAACTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGTGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa                                                                        <  1:379190/143‑1 (MQ=255)
                                                                           aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                                                                <  1:950745/143‑1 (MQ=255)
                                                                                   cAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTg                                                        <  1:700817/143‑1 (MQ=255)
                                                                                        cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagca                                                   <  1:342590/143‑1 (MQ=255)
                                                                                            ctgctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAg                                               >  2:418306/1‑143 (MQ=255)
                                                                                              gctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaa                                             <  2:708376/143‑1 (MQ=255)
                                                                                               ctTACTGGAAGCGCCCGCCTGGTGCCCAAAGCTCCGAAGGGATAAGCAACGGTAGTGTGCGACCGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa                                            <  1:649729/143‑1 (MQ=255)
                                                                                               ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa                                            <  2:379008/143‑1 (MQ=255)
                                                                                               ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGGTTTCTGCAGCATCAGaaa                                            >  1:1003359/1‑143 (MQ=255)
                                                                                                 tACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTGTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgt                                          >  1:666979/1‑143 (MQ=255)
                                                                                                    tGGCAGCGCCCGCCTGGTGGCTAAAACTCCGAAGGGATGATCAACGGTAGTGGGCGAACGCCTTGTTGGCTTCGGCCATACGGTGTACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcg                                       <  2:978057/143‑1 (MQ=255)
                                                                                                    tGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcg                                       <  2:907881/143‑1 (MQ=255)
                                                                                                    tGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTATTCTGCAGCATGCGaaaactcg                                       >  2:1017683/1‑138 (MQ=255)
                                                                                                     ggAAGCGCCCGCCTGGTGACTAAAACTCCGAATGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTACCTGCAGTACCTTTGTTTTCTGCCGCATCAGAAAgttcgt                                      >  2:578201/1‑143 (MQ=255)
                                                                                                        tctgtccgccTGGTGAGTAGAGCTCTGATGTGATAATCTAGCGTAGTGTGCGAACGCCTTGTTTGCTTCGGCCATACGGTGTTCGTCTTCACGTTTCTTATCTGCATTACCTTTGTTTTCTTCAGCATCAGAAAGttcgttcg                                   <  2:322161/138‑1 (MQ=255)
                                                                                                              cgccTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATTCGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGgc                             <  2:278062/143‑1 (MQ=255)
                                                                                                                   tgTGACTAAAGCTGCGCGGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGGTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCaga                        <  2:180890/142‑1 (MQ=255)
                                                                                                                   ccttaccaaaaCTCCGAAGCGATAACCAACCGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCaga                        <  1:554154/136‑1 (MQ=255)
                                                                                                                        cTAAATCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCAt                   <  1:746925/143‑1 (MQ=255)
                                                                                                                        cTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCAt                   >  1:618330/1‑143 (MQ=255)
                                                                                                                           aaaCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGa                >  2:33434/1‑143 (MQ=255)
                                                                                                                           aaaCTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCGTCGGCCATACGGTGAACGTGTTCACGTGGCTGAACTGCAGGACCTGTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGa                <  1:187469/143‑1 (MQ=255)
                                                                                                                                  gAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCa         <  2:145187/143‑1 (MQ=255)
                                                                                                                                  gAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTCTCTGCAGCATCAGCAAGTTCGTTCGCCAGGCGCAGCGCCATCGATTTATCa         >  2:1000686/1‑143 (MQ=255)
                                                                                                                                         tAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCAATAGACAGGCTCTGAGCCATGGATTTATCACCGCGtt  >  1:748621/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CCGATGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTT  >  NC_000913/3473474‑3473753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑