Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,515	G→A	6.3%	T247M (ACG→ATG)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,515	0	G	A	6.3%	170.1 / 2.7	64	T247M (ACG→ATG)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (23/37);  new base A (2/2);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

GCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATAT  >  NC_000913/4241379‑4241653                                                                                                                                         |                                                                                                                                           gCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGc                                                                                                                                      >  1:539786/1‑143 (MQ=255) gCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGAAAAGGGGGTGTGCAACCATGAGCCAGGGGGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGc                                                                                                                                      >  1:807647/1‑143 (MQ=255)    gACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCa                                                                                                                                   >  1:649601/1‑143 (MQ=255)       aaaTTGCTGGAAGATGGAGAGCACTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATaa                                                                                                                                <  1:1079952/143‑1 (MQ=255)          ttGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATAAGCAGACGACCACCCGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTCGCAAGCGTGTTACCCaacatgc                                                                                                                             >  2:166763/1‑137 (MQ=255)           tGCTGGAAGATGGCGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAAtttt                                                                                                                            <  2:844075/143‑1 (MQ=255)           tGCTGGAAGATGGAGAGCATTACGCCGATTACAATCCCGCCCAGGCCAACCATCAGCAGACGACCACCGGTTTTGCGGCTATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAAtttt                                                                                                                            <  2:189764/143‑1 (MQ=255)            gCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTCCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                                                                                           >  1:139616/1‑143 (MQ=255)                gAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACACCACCAGACCACCTGCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCa                                                                                                                       <  1:924683/143‑1 (MQ=255)                 aaGACGCAGAGCATTACGCCGATTACTCTCACGCCCACGCCGAACTTCAGCAGACGACCACCGGTCTTGCGGCCATGTTCCAGGTAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATACTTTTGCGCAg                                                                                                                      <  2:781539/143‑1 (MQ=255)                       gagaGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCCTACCACGCCGAGTGGCGTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                                                                                                >  1:509917/1‑143 (MQ=255)                                 cGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGt                                                                                                      >  1:539724/1‑143 (MQ=255)                                  gCCGATTACAATCACGCCCACGGCAAACATCAGGAGCCGACGACCTGTTTTGCGGCGATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGtt                                                                                                     <  2:929073/143‑1 (MQ=255)                                   ccGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTc                                                                                                    <  1:19371/143‑1 (MQ=255)                                      aTTACCATTACGCCCACGCTAATCATCAGCCGACCACCACCGTTTCTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttg                                                                                                 <  1:205330/143‑1 (MQ=255)                                      aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCACGCGTGTTGCCCATAATTTTGCGCAGGATACCCTCCGCCTGTTcttg                                                                                                 <  2:807647/143‑1 (MQ=255)                                       ttACAACCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgc                                                                                                <  1:295857/143‑1 (MQ=255)                                        tACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgct                                                                                               <  2:183860/143‑1 (MQ=255)                                         aCAATCACGCCCACGCCAAACATCAGCAGACGACCAACGGTTTTGCGGCAATGATACAGGGAGTGTTTAATTTCCGTTACTGCCGGAGTTGCAATCGAGTCGCCAACAATCTTGATAAGGAAACGTTCCGCCTGGTcttactt                                                                                              >  2:382941/1‑143 (MQ=255)                                           aaTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgcacgc                                                                                            >  2:412031/1‑139 (MQ=255)                                             tCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGGTACCTTCCGCCTGCTCTTGCCTGCcg                                                                                          >  2:163000/1‑143 (MQ=255)                                               acgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCATGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                                                                                        >  2:1038203/1‑143 (MQ=255)                                               acgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCATGTTGCCCATAATTTCGCGCAGGATACCTTCCGCCTGCTCCTGCTTGCcgcg                                                                                        >  2:1038947/1‑143 (MQ=255)                                                cgcccacgccAAACATCAGCAGACGCCCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcgc                                                                                       <  2:868948/143‑1 (MQ=255)                                                           aaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGGGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagcca                                                                            <  2:271948/143‑1 (MQ=255)                                                               tCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGACGGTTTAATTTCCTGTACTGCCCGAGTTCCAAGCGCGTTGCCCATAATTTCGCGCCGGCTACCTTCCGCCTGTTCTTGCTCGCCCCCCGCCCCCAGCCACCGa                                                                        >  1:518753/1‑143 (MQ=255)                                                               tCAGCAGACCACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGa                                                                        <  2:687160/143‑1 (MQ=255)                                                                 agcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGGTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAgg                                                                      <  2:682230/143‑1 (MQ=255)                                                                   cagACGACCACCGGTTTTGCGGCCATGATCCAGTGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGAc                                                                    <  1:288069/143‑1 (MQ=255)                                                                   cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTCCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTCTTCCTGCTCACCGCGCGACCTccgccagccgacac                                                                    >  2:1001821/1‑137 (MQ=255)                                                                    agACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACt                                                                   >  2:892714/1‑143 (MQ=255)                                                                         acgtcctgTTTTGCGGGCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAAGTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTg                                                              <  2:55123/136‑1 (MQ=255)                                                                          ccaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCCGGATACCTTCCGCCTGTTCTTGCTTACCACGCGACATCAGCCCCCGCGGCCCTTCTgg                                                             >  2:1090642/1‑143 (MQ=255)                                                                            accGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTTCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCACCGAGGACTTTCTGGcc                                                           >  2:373989/1‑142 (MQ=255)                                                                            accGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGCCATCAGCCAGCGAGCACTTTCTGTCa                                                           >  1:864793/1‑143 (MQ=255)                                                                            accGGTTTTGCGGCCATGATACACGGAGTGTTTAATTTCATGTACTGCCTGAGTTGCAAGCGTGTTGCGCATAATTTTGCGCAGGCTACCTTCAGCCTGTTCTTGCTTGTCGCGCGACATCGCCCAGCGAGAGCCTTCTgacg                                                           >  2:1092095/1‑140 (MQ=255)                                                                              cGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGATTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGTGGACTTTCTGGCACg                                                         >  1:70816/1‑143 (MQ=255)                                                                              cGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACg                                                         <  2:753299/143‑1 (MQ=255)                                                                                gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGt                                                       <  2:649601/143‑1 (MQ=255)                                                                                      ctgctaTGATCCAGGGAGTGTTTATTTTCCTGTACTGCCTGCGTTGCAAGTGTGTTGCCCATAATTGTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcag                                                 <  2:556819/138‑1 (MQ=255)                                                                                      cGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcag                                                 <  1:849484/143‑1 (MQ=255)                                                                                        gCCATGATCCAGGGCGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagca                                               <  1:232370/143‑1 (MQ=255)                                                                                          cATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACATTCTGGCACGCTATACagcagc                                             >  2:16875/1‑143 (MQ=255)                                                                                             gATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCATGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCAtt                                          <  1:1038947/143‑1 (MQ=255)                                                                                             gATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCATGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCAtt                                          <  1:1038203/143‑1 (MQ=255)                                                                                               tCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTaa                                        <  1:621364/143‑1 (MQ=255)                                                                                                ccAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAg                                       <  1:450423/143‑1 (MQ=255)                                                                                                  cgggAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGaa                                     <  2:509917/142‑1 (MQ=255)                                                                                                  aGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGAATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGaa                                     <  1:556919/143‑1 (MQ=255)                                                                                                    ggAGTGTTTAATTTCTGGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGTGCTACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAAca                                   <  1:98899/143‑1 (MQ=255)                                                                                                             aggctCCTGTACTGCCTGAGTTGCGAGCGCGGGGCCCATAATGGTGCGCAGGATACCTTCCGCCTGTTCTCGCGCGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAg                          <  2:1084863/139‑1 (MQ=255)                                                                                                              atgtcctgtcctgCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAgg                         <  2:563489/140‑1 (MQ=255)                                                                                                              aTTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAgg                         <  1:163000/143‑1 (MQ=255)                                                                                                               tttCCTGCACCTCCTGTCTTGCATGCGTGTTGCCCATAATTTTGCGCAGGATACCCTCCCCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTCTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAggg                        <  1:61094/143‑1 (MQ=255)                                                                                                                    tGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTACGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGACCGGTATACAGCAGCATTAAGCACAGCAGTGCAgggcgaca                   >  2:1091354/1‑138 (MQ=255)                                                                                                                          gCCTGAGTTGCAAGCGTGTTGCCCATAATTTTCCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGa             <  1:362095/143‑1 (MQ=255)                                                                                                                           ccggAGTTGCGAGCGTGGTGCCCATAATTTTGCGCAGGCTACCTTCCGCGTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAg            <  1:884537/140‑1 (MQ=255)                                                                                                                           ccTGGGTTGCAAGCGTGTAGACCATAGTGTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAg            <  2:268070/143‑1 (MQ=255)                                                                                                                            cTGAGTTGCAAGCGTGTTGCCCATAATTCTGCGCAGGATACCTTCCGCCTGTTCTTGCAAGCAGCACGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAgg           <  1:412031/143‑1 (MQ=255)                                                                                                                               aGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCCTTAAGAACAGCAGAGCAGGGATACATCCCGAGGCac        >  2:928515/1‑142 (MQ=255)                                                                                                                                 ctGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAAc      <  1:250118/142‑1 (MQ=255)                                                                                                                                   gCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGCAATATCTTCCATGGTATACATCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACat    <  1:1101552/143‑1 (MQ=255)                                                                                                                                    cAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACata   >  1:819381/1‑143 (MQ=255)                                                                                                                                     aaGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGAGATACATTCCGAGGCAAACatat  >  2:399012/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                           GCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATAT  >  NC_000913/4241379‑4241653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑