| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,366,854 | T→G | 6.7% | intergenic (‑81/+37) | dcuA ← / ← aspA | C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,366,854 | 0 | T | G | 6.7% | 151.7 / 4.2 | 60 | intergenic (‑81/+37) | dcuA/aspA | C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base T (29/27); new base G (2/2); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-02 | |||||||||||
CCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACC > NC_000913/4366713‑4366992 | cccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTGCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc < 1:909761/143‑1 (MQ=255) ccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACACGACAAAAGGCAAGTCATCt > 1:612138/1‑143 (MQ=255) ccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCt > 2:446532/1‑143 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTg > 1:144445/1‑143 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTCGTTTTTTAACATGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAACGGCACGTCATCTg > 1:808194/1‑143 (MQ=255) ccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGCTCTACAACTAGCCTATTAGCCTTCCTTGTCTTTTGACAAGTTGAGATTAGATTGTTATTTTTAAGTTACTACTAACACTAAAACAGACACGTCATCAGa > 2:13955/1‑143 (MQ=255) aTCGGGCGACCAGGAAGATCGCCAGCAAAACTATGATGGATTCTACAACGAGCATATTAGCCTTCCTTGTGTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCAAAAGAAAAAAGGCACGTCATCTGACGt < 2:297547/143‑1 (MQ=255) cgcCCAAGAAGCTCGCCAGCAAAACTCTGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAACTTGTTAGTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt < 1:696999/143‑1 (MQ=255) gcCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCtttt < 1:594319/143‑1 (MQ=255) ccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCCTATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCtttttt < 2:554926/143‑1 (MQ=255) cAAGGAGATCGCCTGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCttttttc < 2:315706/143‑2 (MQ=255) cAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGGAAAAAGGCACGTCATCTGACGTGCCTTTTTTa < 2:672377/143‑1 (MQ=255) aagaagATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAt > 1:66067/1‑143 (MQ=255) agaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACACGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTattatt > 2:206005/1‑143 (MQ=255) agaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAtt > 1:618685/1‑143 (MQ=255) agaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCTTTTTTCtt < 1:666874/143‑1 (MQ=255) gCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATCTTAGATTGTTATTTTTAAGTTACTGCTCCCAAGAAAAAAGGCACGTCATGTGACGGGCCGTTTTTTTTTGtactac > 2:825324/1‑143 (MQ=255) gCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtactac > 1:9646/1‑143 (MQ=255) atgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGCAAAAAGGCACGTCATCTGACCTCGCTTTTTTATTGGTAATCCCGTGTACGCTTACTg > 2:167493/1‑143 (MQ=255) atgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTg > 1:382272/1‑143 (MQ=255) ttCTACAACTAGCATATTAGCCTTCCTAGTTTTTTAACAAGATGATATTAGATCGTTATTTTTAAGTTACTCATCACAAGAAAAGAGGCACGACATCTGACGTGCCTCTTTTATTTGTACTACACTGTACGATTACTATTCGc > 2:150360/1‑143 (MQ=255) tCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCt > 1:1028291/1‑143 (MQ=255) cTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACACTGTACGATTACTGTTCGCtt > 2:547586/1‑143 (MQ=255) aCAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtc < 1:927938/143‑1 (MQ=255) cAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtca < 2:302253/143‑1 (MQ=255) aaCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat < 1:574032/143‑1 (MQ=255) aaCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat > 1:26279/1‑143 (MQ=255) aaCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat > 1:563479/1‑143 (MQ=255) cgaGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatca < 2:704305/141‑1 (MQ=255) cgaGCATATTAGCCTGTCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatca < 1:1054583/141‑1 (MQ=255) cTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAAACTATACGATTACTGTTCGCTTtcatca < 2:396364/143‑1 (MQ=255) tAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAg > 1:738334/1‑143 (MQ=255) cATATTAGCCTTCCTTGTTGTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGtat < 2:630911/143‑1 (MQ=255) tccctGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGCACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCt < 1:192579/143‑1 (MQ=255) tGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTATACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGt > 1:78960/1‑143 (MQ=255) tAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTGTAGCGTTTTGCTTTGTAAGCCgg > 1:305412/1‑143 (MQ=255) aaGCTGATCTTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTCGACACGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGc < 1:696700/143‑1 (MQ=255) gTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCAt > 1:1027176/1‑143 (MQ=255) tGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCa < 1:446532/143‑1 (MQ=255) aGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGGGCATCAGATTCTg > 1:464340/1‑143 (MQ=255) gATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCTTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt < 2:239150/143‑1 (MQ=255) ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGga > 1:46786/1‑143 (MQ=255) ttttAAGTTTCTGCTCACAAGAAAAAAGGCACGTCATCTGCCGTGCCTTTTTTATTTGTCCTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACTGAGaaa < 2:547737/143‑1 (MQ=255) tttAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTAGCTTTCATCAGTATAGCGTTTTGCTTTGTACCCCGGGTGCATGAGATGCTGTACGGAGaaaa > 2:774920/1‑143 (MQ=255) tAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGCAAata > 2:1050863/1‑143 (MQ=255) caAGAAAAACGGCACGTCATCTGACGTGCCTTTTGTATTTGTACTGCCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCGTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGc < 2:57482/143‑1 (MQ=255) aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATTTACCGCTATACGGAGAAAATATCGTCAGGTTCCGCt > 2:228728/1‑143 (MQ=255) cgaaaaGGCACCGCCTCTGACGTGCCTTTTTTATTTGTACTAACCTGGACGTGTACTGTTCGCTTTCATCATTATTGCGTTTTTCTCTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCa < 1:206005/141‑1 (MQ=255) aaaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCa < 1:928593/143‑1 (MQ=255) aaaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGATCGGTTTCATCAGTATAGCATTTTGCTTTGTCAGCCGGGTGCATCAGATTATGTACGGAGAAAATGTAGTCAAGTACCgagtaa > 2:635340/1‑138 (MQ=255) aaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTACAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc < 2:563479/143‑1 (MQ=255) aGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCaa < 2:923000/143‑1 (MQ=255) aGGCACGCCATCTGACGTGCCTTTTTTATTTGTGCTACCCGGTGCGATTACCGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGCTTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCaa < 2:178910/143‑1 (MQ=255) ggCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAAc > 1:1029899/1‑143 (MQ=255) ggCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGCGAAAATATCGTCAAGTTCCGCTTCAGTCAAc > 1:648446/1‑143 (MQ=255) gCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCAGCAGATTCTGTACGGAGAAAATATCGTCAAGTACCGCCTCAGTCACCa > 2:947509/1‑143 (MQ=255) gCACGCCATCTTACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGCGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACa < 1:434923/143‑1 (MQ=255) cGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGAc < 2:46786/143‑1 (MQ=255) gTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGAcc < 1:614710/143‑1 (MQ=255) gTCATCTGACGTGCCTTTTATATTAGTACAACCTTGTACGATTATTATTCGCTTTCAACAGTATAGAGTTATGCTTTGTAAGCCGGGTGCATCAGAATCTGTACGGAGAAAATATCGTCAAGTTACGCATCAGTCAACAAAcc > 2:133814/1‑143 (MQ=255) | CCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACC > NC_000913/4366713‑4366992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |