Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,640,231 C→A 72.0% E26* (GAA→TAA)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,640,2310CA72.0% 84.2 / 31.7 62E26* (GAA→TAA) arcADNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (8/10);  new base A (22/21);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCA  >  NC_000913/4640089‑4640373
                                                                                                                                               |                                                                                                                                              
gCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAACACATCATAGCCTTc                                                                                                                                                >  2:592217/1‑143 (MQ=255)
 ccTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTcc                                                                                                                                               >  2:46604/1‑143 (MQ=255)
   tGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGc                                                                                                                                             <  1:573139/143‑1 (MQ=255)
    gCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGGCATATTCAGAGAGGATCTGATGCATTTCCGCGCCCTCCGTCGCTTC‑GCAACCATCATAGCCTTGCGCt                                                                                                                                            >  1:226146/1‑143 (MQ=255)
       cgcgCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCCATCGCTTC‑GAAAACATCATAACCTTcacctccg                                                                                                                                         >  2:1107068/1‑136 (MQ=255)
         cgcAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGaa                                                                                                                                       <  1:398093/143‑1 (MQ=255)
          gcAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGaaa                                                                                                                                      <  1:195585/143‑1 (MQ=255)
             gTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTA‑CAAAACCTCATAGCCGCACGCCCCGAAAATa                                                                                                                                   >  2:382002/1‑143 (MQ=255)
              ttCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATAc                                                                                                                                  >  2:562862/1‑143 (MQ=255)
                cACGCGCTGACAGAAGACCGTGCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGaatttcgaa                                                                                                                                <  2:161472/143‑8 (MQ=255)
                cACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGaatttcgaa                                                                                                                                <  2:699266/143‑8 (MQ=255)
                 aCGCGCTACCAGAAGACCGTTCTCACCCGGCAGATTGATATCCATGATCACCATGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACttt                                                                                                                               <  2:434487/143‑1 (MQ=255)
                 aCGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACttt                                                                                                                               >  2:405747/1‑143 (MQ=255)
                 aCGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACttt                                                                                                                               >  2:413775/1‑143 (MQ=255)
                  cgcgCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGCTCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATACCCTTCCGCCTCGAAAATACtttt                                                                                                                              >  2:466499/1‑143 (MQ=255)
                         aCAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCCTTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtg                                                                                                                       >  2:551148/1‑143 (MQ=255)
                         aCAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtg                                                                                                                       <  1:932891/143‑1 (MQ=255)
                           agaagaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGtt                                                                                                                     <  1:334061/143‑1 (MQ=255)
                           agaagaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCATCCTCTTCGAAAACACTTGTCTACGTGtt                                                                                                                     >  2:265162/1‑143 (MQ=255)
                            gaagaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGACGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGCAACTACTTTTCAACGTGTTg                                                                                                                    >  1:870869/1‑143 (MQ=255)
                             aagaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc                                                                                                                   <  1:963826/143‑1 (MQ=255)
                             aagaCCGCGCTGACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc                                                                                                                   <  1:218625/143‑1 (MQ=255)
                                aCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCCACGGTTTGCggg                                                                                                                >  1:977962/1‑141 (MQ=255)
                                   gTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTa                                                                                                             >  1:669100/1‑143 (MQ=255)
                                       ttgcccGGCAGATTGATAGCCATGAGGAGCGGGGTGAGGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGGTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa                                                                                                         <  1:898368/140‑1 (MQ=255)
                                           ccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGGCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg                                                                                                     >  1:819937/1‑143 (MQ=255)
                                            cGGCAGATTGATATCCATGATCGCCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGt                                                                                                    <  2:762137/143‑1 (MQ=255)
                                              gCAGATTGATAGCCATGATCACCAGGTTGGTGTCATATTCAGAGAGGCTCTGATGCAGTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTGACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCt                                                                                                  <  2:531649/143‑1 (MQ=255)
                                                  aTTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTGCCCACTCGTCTTCaa                                                                                              >  1:176252/1‑143 (MQ=255)
                                                    ggataggcATGATCACCGGGTTGATGTCGTGTTCAGGGGGGATCTGGTGCATTTCCGCGCCATCTGTCGCTGC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACg                                                                                            <  2:344503/136‑1 (MQ=255)
                                                      atatCCATGATCACCAGGTTGATGTCATATTCAGAGCGGATCGGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGAt                                                                                          <  1:624214/143‑1 (MQ=255)
                                                         tCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTGGTCTTCACCGATAAg                                                                                       >  2:950249/1‑143 (MQ=255)
                                                           cATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGaa                                                                                     <  2:471612/143‑1 (MQ=255)
                                                            aTGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAt                                                                                    <  2:661218/143‑1 (MQ=255)
                                                              gATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTTCCATCTTGTCTTCAACGATAAGAAtgt                                                                                  <  2:396104/143‑1 (MQ=255)
                                                                 cACCAGGTTGATGTCATATTCAGAGAGGATCGGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCg                                                                               <  1:298240/143‑1 (MQ=255)
                                                                   ccAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCggg                                                                             <  1:880967/143‑1 (MQ=255)
                                                                         tGATGTCATATTCAGAGAGGATCTGATGCAGTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTGCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGc                                                                       <  1:892231/143‑1 (MQ=255)
                                                                             gTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt                                                                   >  1:624700/1‑143 (MQ=255)
                                                                             gTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt                                                                   >  2:285414/1‑143 (MQ=255)
                                                                               cATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GACAACATCATAGCCTTACGCTTCGAAAATACTTTTCCACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGACTGTGCGGGCTCTGCATGttt                                                                 >  2:15096/1‑143 (MQ=255)
                                                                                atatTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGTTGGGGACCAACTTGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg                                                                >  1:754763/1‑143 (MQ=255)
                                                                                 tatTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGATATTTCTTTTCTACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGc                                                               <  1:36441/143‑1 (MQ=255)
                                                                                          agGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAACCATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCACCGATAAGACTGTGCGGGGTCCGCATGTTTGCTACCTACAt                                                      >  2:151266/1‑143 (MQ=255)
                                                                                                ttttGCATTTCCGCCTCATCTGTCTCTTCTCGAGTCAT‑ATATCGTTCCGCTTCGAAATTACTTTTCAACGTGTTGCGTGTTACCATCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCaa                                                <  2:778117/140‑1 (MQ=255)
                                                                                                tGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGATGCGTGTTACCAACTCGTCTTCAACGATAACAATGTGCGGAGTCTGCATGTATGCTACCTAAAATGCCaa                                                >  2:291647/1‑143 (MQ=255)
                                                                                                tGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATAC‑TTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAAc                                               >  1:228279/1‑143 (MQ=255)
                                                                                                   tGCATTTCCGAGCCATCTGTCGCTTC‑GAAGACGTCATAGCCTTCCTCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTa                                             <  2:66365/143‑1 (MQ=255)
                                                                                                    gCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaa                                            >  1:476385/1‑143 (MQ=255)
                                                                                                         tCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGa                                       >  1:749193/1‑143 (MQ=255)
                                                                                                           cgcgCCATCTGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                                     >  2:316632/1‑143 (MQ=255)
                                                                                                                    tGTCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTACATTGCCAACTAAATCGAAAAAGGAAGTa                            >  1:704919/1‑143 (MQ=255)
                                                                                                                     gTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTAc                           <  1:643978/143‑1 (MQ=255)
                                                                                                                      tCGCTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACa                          >  2:287105/1‑143 (MQ=255)
                                                                                                                         cTTC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCCACGATCAGAATTTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaaaa                       <  1:416007/143‑1 (MQ=255)
                                                                                                                          ttC‑GAAAACATCATAGCCTTAGGCTGCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGACAACTAGATCGAAACAGGAAGTACAAAAg                      >  2:291963/1‑143 (MQ=255)
                                                                                                                          ttC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAGGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAg                      >  1:529115/1‑143 (MQ=255)
                                                                                                                           tC‑GAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTCCAAAAGt                     >  1:463817/1‑143 (MQ=255)
                                                                                                                                  ccaccatAGCGTTACGCTGCGAAAATACTTTTCAGCGTGTTGCGTGTTACCACCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAACTGCCAACTAAATCGAAACAGGAAGAACAAATGTCCCTGa               <  2:973046/139‑1 (MQ=255)
                                                                                                                                  aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGa               <  1:972341/143‑1 (MQ=255)
                                                                                                                                   catcatTGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAc              <  2:754763/143‑1 (MQ=255)
                                                                                                                                   catcatAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTACGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTCAATCGCAACAGGAAGTACAAAACGCCCTGAc              >  2:903675/1‑143 (MQ=255)
                                                                                                                                       atAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgc          <  1:382002/143‑1 (MQ=255)
                                                                                                                                         aGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGCTTGCTACCTAAATTGCCAACTAAATAGAAACAGGAAGTACAAAAGCCCCTGAcctgcca        >  2:89045/1‑142 (MQ=255)
                                                                                                                                           ccTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGa      <  1:667700/143‑1 (MQ=255)
                                                                                                                                               ccGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatgcc  >  1:695699/1‑142 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTC‑GAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCA  >  NC_000913/4640089‑4640373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑