| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 546,991 | G→C | 10.0% | Q104H (CAG→CAC) | fdrA → | putative acyl‑CoA synthetase FdrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 546,991 | 0 | G | C | 10.0% | 89.3 / 3.1 | 36 | Q104H (CAG→CAC) | fdrA | putative acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base G (11/21); new base C (2/2); total (13/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
ATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATCGCAACCTCAACGTGATGATGTTCTCCGAT > NC_000913/546849‑547126 | agaaCGCCACGCCGAACGATGTTTGCGGGGCAAGTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATGATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGCCGAAg < 2:193485/141‑1 (MQ=255) cgcccggccGACCGATATTTGCGTGCAAATTAGTATCGGGGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGc < 1:203019/137‑1 (MQ=255) cgccacgccGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCACGTGc > 2:102539/1‑143 (MQ=255) acgccGAACGAGATGTGCGTGGGAATTCGGAGCGAAGCGGCGGATGGGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCg < 1:764445/143‑1 (MQ=255) ggccGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCACCTGCGTcaa < 1:604722/142‑3 (MQ=255) cGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTgg < 1:661282/143‑1 (MQ=255) cGAACGATAGTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGAGTATGCAGCAGCTTGAAGAGGCGCTAAAGCAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTgg < 2:761369/143‑1 (MQ=255) gAACGATATTTTCGTAGCAAAACGTAGCGAAGCCGCGGATGCGGGGATCGCCCAGCCGATTATACATCAGCTCGCAGAGGCGCTAAAACAACTGCCCCACGGGTCAAGCAGCACACCCCCCTTGACGCAGGGCCGGCGCTggc > 2:75520/1‑142 (MQ=255) aaCGATATTGGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCACCTGCGTcaacgcct < 2:1092247/143‑8 (MQ=255) ttGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCt < 2:66990/143‑1 (MQ=255) tggCGTGGCAATTGGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCt < 2:1085104/141‑1 (MQ=255) gCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGaa < 1:1034434/143‑1 (MQ=255) gCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGaa < 1:351504/143‑1 (MQ=255) cAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGaaa < 1:69719/143‑1 (MQ=255) aaTTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCGTGACCGaaag > 2:130153/1‑142 (MQ=255) gggcgcAGCGGCGGAGGCGGGGATCGCGCAGGCGACTAGGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCg < 2:329353/141‑1 (MQ=255) ggcgcgtGCGGGGATCGGGCAGGCGAGTATGAAGCAGCTTGAGGAGGCGGTAAAGCAACGGCCGAAGGGGTCAGGGCGCAGGCGGGCGTTGACGCAGGTGCGGCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATc < 2:802628/137‑1 (MQ=255) cggATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTg < 1:550883/143‑1 (MQ=255) cGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTg > 1:664167/1‑143 (MQ=255) ggggATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGa > 1:27061/1‑143 (MQ=255) gATTATGCAGCAGCTTGAAGAGGCGCTAAAACACCTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTTACGTTGGTGTGTCGCTAAGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCg < 1:819681/143‑1 (MQ=255) gttgtGCAGCAGCTTGAAGCGGCGCCAAAGCAAGTGGCGCGGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGa < 2:490837/139‑1 (MQ=255) aTTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGa < 1:711659/143‑1 (MQ=255) tGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGCCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTAt > 1:991189/1‑143 (MQ=255) gcagcTTGAAGAGGCGCTAAATCAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATgcgg < 2:913539/143‑1 (MQ=255) ggagGGGCCAGAACTACTGGCGCGGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACATTGGCTGTCAGGAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGTCGAGTATGCGGCGGAGCTgg < 2:977074/142‑1 (MQ=255) agGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCa > 1:553747/1‑143 (MQ=255) gcgcTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCaaa > 1:453303/1‑143 (MQ=255) tAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAg > 1:888960/1‑143 (MQ=255) aaaaCAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGCCTGGCGAGTATGCGGCGGAGCTGGCAAACCAgg > 2:26669/1‑143 (MQ=255) ctgccccAGGGGTCAGGCAGACGCCAGGCGTTGACGCAGGTGCGTCGCGGGGACAGTGCCTGTCAGAAATTACCCCATGCAACTCTGGCTTTGATTTCAGTGGCTGGCGAGGATGCGGCGGAGCTGGCAAACCAGGCGCTGGa < 1:304646/137‑1 (MQ=255) ggCGCAGGGGTCAGGCAGCAGCCAGGCGCTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATc < 1:81419/143‑1 (MQ=255) cAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTAGGCGGCGGAGCTGGCAAACCAGGCGCTGGACCGCaa > 2:185726/1‑143 (MQ=255) cagcagCCAGGCGTTGACGCAGGGGCGTCGCTGGGACAGTGCCTGTCAGCAATTACCCGATGCCCATATGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGATGGCACACACGGCGAAGGAGCGCAACCTCAACGtga > 2:9312/1‑143 (MQ=255) agcagcCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATCGCAACCTCAACGtgat < 1:662573/143‑1 (MQ=255) ttGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATCGCAACCTCAACGTGATGATGTTCTCCGa < 1:353652/143‑1 (MQ=255) cgACGCACCTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATGTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATCGCAACCTCAACGTGATGATGTTCTCCGAt > 2:943986/2‑143 (MQ=255) | ATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCAGGGGTCAGGCAGCAGCCAGGCGTTGACGCAGGTGCGTCGCTGGGACAGTGCCTGTCAGAAATTACCCGATGCCAATCTGGCGCTGATTTCAGTGGCTGGCGAGTATGCGGCGGAGCTGGCAAACCAGGCGCTGGATCGCAACCTCAACGTGATGATGTTCTCCGAT > NC_000913/546849‑547126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |