| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,470 | C→T | 100% | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,470 | 0 | C | T | 96.4% | 114.7 / ‑4.2 | 42 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (24/15); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC > NC_000913/696333‑696607 | cGCATCCGACATTGAAGGATACGACGTGGCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGTAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAt < 2:641036/143‑1 (MQ=255) cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATcc > 1:581377/1‑143 (MQ=255) cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg > 1:530632/1‑143 (MQ=255) gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTGGAATCCGGGGc > 1:420552/1‑143 (MQ=255) gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc > 1:630309/1‑143 (MQ=255) aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg > 1:975939/1‑143 (MQ=255) aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg > 1:871358/1‑143 (MQ=255) gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGGGGGTGGGGTGCGAGGATGCGAACCTCGGAATGCCGGCATTCGAATCCGGTGGCGGACCGCtt > 1:373592/1‑143 (MQ=255) aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt > 2:1088089/1‑143 (MQ=255) cGTGTCAACGTCGCATTCGTCATTGAATGATCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa < 2:698819/143‑1 (MQ=255) gtcgagaTCTCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTGGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc < 2:946801/137‑1 (MQ=25) tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAACCCGGTGCCGTACAGCATGGCAAtcgcgc > 2:712627/1‑138 (MQ=37) gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt < 2:1043568/143‑1 (MQ=255) ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGCGGATTCGAACCTCGGAATGGAGGAATTAAAATCCGGTGCCTTGCCGCTTGGCGATACCCCAACTGGGTGCa > 1:42253/1‑143 (MQ=37) tCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCACCGGGGTGCAc > 1:385302/1‑143 (MQ=255) gcgtctttGTATGGACTCAGAAGAGCAATACGTTGGCCGAAGCGAGCTTTTTTAATGCGGGTGGTGTACGAGGGTTCGAGCCTCGGAATGCCGGATTTAGTATCCGGTGCCTTACCGCTTGGCGATACCCCAAATGGGTGCAc < 2:849075/137‑1 (MQ=12) tgaatgagCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg < 2:796179/143‑1 (MQ=255) cgcaggaaCGCACAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg < 2:420552/138‑1 (MQ=255) atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACCAGGTa > 1:152778/1‑143 (MQ=255) atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCGGGTGCACTTACAAGGTa > 2:235804/1‑143 (MQ=255) aCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAGAAGGtgcgcg > 2:1002714/1‑138 (MQ=255) aaaaGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa < 1:781181/143‑1 (MQ=255) aaaGCAAGAAGCTCGCCGAAGCGTGCTTTTTTAATGTGGCTGGGGTTCGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGaa < 2:638239/143‑1 (MQ=255) catggtCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGTTTCGAACCTCGGAATGCCGGGATTAGATTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGCGTGCACTTACTAGGTAAGCGTCTTGAATAAAT‑Tg < 2:334205/138‑1 (MQ=21) tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTgg > 1:434736/1‑143 (MQ=255) gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGTGCACTTGCACTGTAAGCGCCCTGGAGCAAT‑GGGCTggga > 2:583145/1‑142 (MQ=17) gAAGCGATCTGTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGTACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTAc < 2:775295/143‑1 (MQ=255) gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGACTAAAG‑TGGCTGGGGTAc > 1:428709/1‑143 (MQ=255) agctgcgttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGAt < 1:601434/136‑1 (MQ=255) aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGGAGAATCCGGTGCCTGACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGAt < 1:251140/143‑1 (MQ=255) ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa > 1:396536/1‑84 (MQ=255) ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa < 2:396536/84‑1 (MQ=40) actgtgGATGGGGTACGTGGATTCGAACCTCGCAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTc < 2:530632/141‑1 (MQ=255) gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGACCCTCGGAATg > 1:289575/1‑143 (MQ=255) aCGGGGACTCGAACCGCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc < 1:857711/143‑1 (MQ=255) aCGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCCGAGTc > 1:407404/1‑143 (MQ=255) aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGCTTCGACCCGCGGCATGCCGCAACCAGa > 1:401230/1‑143 (MQ=25) atgcGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTGCAAGGTCCGCGCATTGAATAAAT‑TGGCTGAGGTACGAGGATCCGACCGTCGGAATACCGGTGTCCGAATc > 2:400339/4‑143 (MQ=18) gagccTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt < 1:231340/140‑1 (MQ=255) cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg < 1:781757/143‑1 (MQ=255) ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGAGTCTTGAATAAAT‑TGGCGGGGGTACGAGGATACGAACCTCGGAATACCGGAATCCGAATCCGCACACTTACCGccaggc > 2:819818/1‑138 (MQ=25) ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGCTGCACTTACACGGCAAGCGCGTCGAATAAATCTGGC‑GGAGTGCGAGGATACGAACCTCGGAAAGCCGGAATCAGACTGCGGTGACCTCCCGCGTGGc > 2:596548/1‑143 (MQ=16) | CGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAT‑TGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC > NC_000913/696333‑696607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |