| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 699,559 | A→G | 8.2% | intergenic (‑382/+15) | asnB ← / ← umpH | asparagine synthetase B/UMP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 699,559 | 0 | A | G | 8.2% | 144.3 / 3.4 | 57 | intergenic (‑382/+15) | asnB/umpH | asparagine synthetase B/UMP phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (20/31); new base G (4/2); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
TGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAA > NC_000913/699424‑699696 | tGCCGGATGCGGCGTGAACGCCTTATCCCGCCTACAAAAGCATGAAAATTCAATATATTGCAGGCGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg > 1:4889/1‑143 (MQ=255)tGCCGGATGCGGCGTGAACGCCTCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATACGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg < 1:314256/143‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGtt < 1:459324/143‑1 (MQ=255) ctgATGCGGCGTGAACGCGTTCTCCGGCCTACCAAAGCCTGAAAATTCAATATATGGCAGGAGCTGCGTAGGCCCGATCAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg < 1:70797/141‑1 (MQ=25) tGCGGCGTGAACGCCTGATCCGGCCGACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAt < 2:598416/143‑1 (MQ=255) ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGATCCAACCACGTCCGCAGACGTGGTTGCTATTCa < 2:240602/143‑1 (MQ=255) ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGCCCGCAGACGTGGTTGCTATTCa > 2:766270/1‑143 (MQ=255) gTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGAt < 2:925253/143‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAAc > 2:740849/1‑143 (MQ=255) cTTATCCGGCCTACAAAAGCATGAAAATGCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGa < 2:435052/143‑1 (MQ=255) ttATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGGACCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAt < 2:1020306/143‑1 (MQ=255) ccGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCa < 1:318353/143‑1 (MQ=255) tggCCTACAAAAGCATGATACTGCGATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg < 2:758770/142‑1 (MQ=255) ggCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGGCTGCTTCGCCTAGCTCATATGGTGCAGCTAGAGG‑‑AGTCATCAGAGCCAACCACGTCCGCAGACGTAGTTGCTATTCAGATAACGTCGATTTCAGc > 1:515005/1‑143 (MQ=255) ggCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGGTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGCTTTCAGc > 1:438079/1‑143 (MQ=255) gCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCg < 1:868732/143‑1 (MQ=255) ccTACAAAAGCATGAAAATTCAATATATTGCAGGGGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGa < 1:74607/143‑1 (MQ=255) ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGa > 2:752464/1‑143 (MQ=255) tACAAAAGCATGAAAATTCAATATATTGCAGGAGCTACGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACt > 1:716858/1‑143 (MQ=255) aaaGCATGAAAATTCAATATATGGCAGGGGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACg < 2:6744/143‑1 (MQ=255) tGAAAATTGAATAGATTGCAGGAGCTGCGTAGGCCTGATAGGCGTAGGGCATTAG‑‑GCTGTGTGGGGTTTGTCAGCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaa < 2:283180/143‑1 (MQ=255) gAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACGATGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAt < 1:157804/143‑1 (MQ=255) gAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAA‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTCTTCAGATAACGTCGATTTCAGCGACTGCCGGGTAAAt > 1:570897/1‑143 (MQ=255) atatatGGCAGAAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTggg < 2:982136/143‑1 (MQ=255) atatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGc > 1:160148/1‑143 (MQ=255) tcttGCAGGGGGTGCGGGGGCGTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg < 2:1009123/141‑1 (MQ=38) ttGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGCGTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAACTCCGGCTGGGGCGGa > 1:469244/1‑143 (MQ=255) gcgggAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa < 2:746439/140‑1 (MQ=255) cAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGAGAACGCCGATTTCAGCGACTGACGGGTAAACCCCGCTGCGGCGGAAAg > 2:159106/1‑143 (MQ=38) aGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAgg < 1:1035079/143‑1 (MQ=255) cGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCagtcggt > 2:359117/1‑137 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGATACGAGTTTGTTATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTc < 1:462698/143‑1 (MQ=39) aGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGa < 1:218813/143‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGatc > 2:195945/1‑142 (MQ=255) gCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGata > 2:264553/1‑143 (MQ=255) gcgtctcgcATCAC‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGc < 2:160148/137‑1 (MQ=255) gCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGc < 1:264553/143‑1 (MQ=255) gcgcATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATACCGTCGCTATCAGCAACTCACAGGTAAATCCAGCTGGGGCGGAAACGCACACTGTCGAGATCGTCCAGatacga > 2:391193/1‑143 (MQ=255) gcATCAG‑‑GCGGTTTGGCGCTTGCCACCAGAGCCACCCACGTCCGCGGACGTGGTTGCTATTCCGATAACGTCGATTTCAGCGCCTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa < 2:929082/143‑1 (MQ=255) cATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGCGCGACGAAac > 1:1043983/1‑143 (MQ=255) cATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAAGCATACTGTCGATATCGTCGAGCGACGAAac > 1:345284/1‑143 (MQ=255) cAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaa < 2:716858/143‑1 (MQ=255) aGTTGGGCGTGTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaaa < 1:766270/143‑1 (MQ=255) tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATCTCAGCGACTGACGGGTACATCCAGCGGGGGCGGAAAGGCATACTGTCGATATCGTCGAGGGAAGAAACACCAGAAAGc > 2:705252/1‑143 (MQ=255) ttGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAAt < 1:752464/143‑1 (MQ=255) tggcatcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATc < 1:756581/140‑1 (MQ=255) tGTCATCAGTGCGAGTCACCGCCGCGGACGGGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAGCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATc < 2:4889/143‑1 (MQ=255) ggTCATCAGGGCCACCCACGTCCGCAGCCGTGGTTGCCATTCAGATAACGCCGATTTCAGCGACGGACGGGTACACCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATc < 1:210287/142‑1 (MQ=255) tcatcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGt > 1:378794/1‑143 (MQ=255) tcaGATCCACTCGCGTCCGGAGAAGTGGTTGCTATTCAGATAACGTCGATTTCTGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGtctc < 2:438079/143‑1 (MQ=255) aatagcAACCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACAAGAAAGCACCAGAATCGCCGCCa > 2:105109/6‑143 (MQ=255) cAACCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATAGAGATGAGGCTGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcct > 2:963323/1‑143 (MQ=255) aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATCTCAGCGACTGACGGCTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGATCGACGCACCACCAGAAAGCACCAGCATCGTCTCCAGAcgtg > 2:749241/1‑143 (MQ=255) aaCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACCCCAGAAAGACCCAGAATCGTCTCGAGAccgg > 1:1057353/1‑141 (MQ=255) accaccTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctgc < 1:1100255/143‑1 (MQ=255) ccACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctacc > 1:1011194/1‑143 (MQ=255) tctgCAGTCGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGGCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGaa < 1:193778/140‑1 (MQ=255) | TGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAG‑‑GCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAA > NC_000913/699424‑699696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |