Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 784,012 T→A 8.5% Q271L (CAG→CTG)  zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,0120TA 8.5% 144.2 / 3.2 56Q271L (CAG→CTG) zitBZn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base T (21/30);  new base A (2/3);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

CGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCA  >  NC_000913/783872‑784150
                                                                                                                                            |                                                                                                                                          
cgcTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCAGGACAAGGTTGATATTCCCTCGGAATGGTGGCGTGCGCAATCTGATAGTGACCCATCAGGTCGTGGGGGATCTGa                                                                                                                                          >  2:644555/1‑143 (MQ=255)
cgcTGTCCCATTAACGGCGATGATGTGAATCGCCACACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGa                                                                                                                                          <  1:699615/143‑1 (MQ=255)
  ccgTCTCATGAATGGGGTGGAGGTGATTGGCCAGACCCCCCTTCGTTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATc                                                                                                                                        <  2:329945/141‑1 (MQ=255)
     tCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGAGGCGCGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCcaa                                                                                                                                     >  1:862689/1‑143 (MQ=255)
       ccATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCAGATCcaaca                                                                                                                                   <  2:712959/143‑1 (MQ=255)
       ccATTAATGGTGATGATGTGAATGGCCAGAAACACCCTCATTTATGTGTCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCcaccc                                                                                                                                   >  1:470706/1‑140 (MQ=255)
        cagtgaTGCTGATGATGTGAATGGCCAGACACACCTTCATTCAGGTGGCAGTGTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCAGATCcaacac                                                                                                                                  <  2:675864/138‑2 (MQ=255)
         aTTAATGGTGCTGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAg                                                                                                                                 <  2:902904/143‑1 (MQ=255)
          ttAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAgg                                                                                                                                <  1:174244/143‑1 (MQ=255)
              tGGTGATGAGGTGAATGGACACACACCCCTGCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATGCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCatc                                                                                                                            <  1:901955/143‑1 (MQ=255)
              gggggctgatgTGAGTGGCCAGGCCCCCGTTCGTTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTTAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCatc                                                                                                                            <  2:609532/137‑1 (MQ=255)
                 tgatgaTGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCatcgtg                                                                                                                         >  1:673409/1‑143 (MQ=255)
                     gatgTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACACGGCatcgtgatcg                                                                                                                     >  1:373606/1‑143 (MQ=255)
                       tgtgAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCatcgtgatcgtg                                                                                                                   >  1:242042/1‑143 (MQ=255)
                       tgtgAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCatcgtgatcgtg                                                                                                                   <  1:938880/143‑1 (MQ=255)
                           aaTGGCCAGACACCCCGTCAGTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGc                                                                                                               <  1:465183/143‑1 (MQ=255)
                              ggCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCggg                                                                                                            <  1:793761/143‑1 (MQ=255)
                               gCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGa                                                                                                           <  1:49178/143‑1 (MQ=255)
                                   gACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCAc                                                                                                       <  1:387261/143‑1 (MQ=255)
                                     cacCCCTTCATTCAGCTGGCCTCCTGGCCCATGCCAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATAAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCt                                                                                                     <  1:287394/143‑1 (MQ=255)
                                      acCCCTTCATTCAGGTGGCAGTCTGGCGCATGACAAGGTGGATATTCCATCTGAATGGTGGCGTGCTCAATCTGCTAGTGATCCATCTGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTg                                                                                                    <  1:625830/143‑1 (MQ=255)
                                       ccccTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGc                                                                                                   >  1:331664/1‑143 (MQ=255)
                                        cccTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGCGATCACCTGca                                                                                                  >  2:380417/1‑143 (MQ=255)
                                         ccTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGcac                                                                                                 >  1:609733/1‑143 (MQ=255)
                                           ttcattcaGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGTCGGGATCACCTGAACAt                                                                                               >  1:965311/1‑143 (MQ=255)
                                               ttcaGGTGGCAGTCTGGCCCATGACAAGGTTGAAATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGCTCCAACAAGGCATCGTGATCGTGAGGCGGGGTCACCAGCACATGCac                                                                                           >  2:187247/1‑142 (MQ=255)
                                                tcaGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACGTGCACGTGCAAc                                                                                          >  1:448679/1‑143 (MQ=255)
                                                  cgttGGCAGTCTGGCCCAGGACAAGGTTGCTATTCCATCCGATTGGTTGCGTTCTCAATCTGCTAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGt                                                                                        <  2:307531/140‑1 (MQ=255)
                                                   ggTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGCTCCAACAAGGCATCGTGCTCGTGTGGCGGGATCACCTGCACATGCAACGtc                                                                                       <  1:773438/143‑1 (MQ=255)
                                                   ggTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCGTCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGtc                                                                                       <  1:502996/143‑1 (MQ=255)
                                                          gTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCAGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCg                                                                                <  1:169481/143‑1 (MQ=255)
                                                                   aTGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATCGTGCGCCAACATGTGGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGc                                                                       <  1:1096802/143‑1 (MQ=255)
                                                                               ttatTGCATCTGGATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAta                                                           <  2:760865/142‑1 (MQ=255)
                                                                               atatTCCAACTTAATGGTGGCGTGCTCAATCTGATAGTGATCCAGCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAta                                                           <  2:654248/143‑1 (MQ=255)
                                                                                 cttgcttCTGAGTGGTGGCGTGCTCAATCTGATAGTGATCCGTCAGGTAGTGTTGGATGTGATCCAACAAGGCCTCGTGGTCGTGTGCCGGGATCACCTGCACATGCAACGTCATCATCGTTTTCTCGCCTACCATCCAtaca                                                         <  2:845134/137‑1 (MQ=255)
                                                                                  ttCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCACCCAtacat                                                        >  2:815215/1‑143 (MQ=255)
                                                                                     cATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTGCCCTCCCtccctgta                                                     >  1:71993/1‑143 (MQ=255)
                                                                                          gAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATgg                                                <  2:662909/143‑1 (MQ=255)
                                                                                                   cGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTg                                       <  1:121029/143‑1 (MQ=255)
                                                                                                    gTGCTCAAGCTGATAGTGATTCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGc                                      <  2:560203/143‑1 (MQ=255)
                                                                                                     tGCTCAATCTGATAGGGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCg                                     <  1:586888/143‑1 (MQ=255)
                                                                                                     agCTCAATCGAATAGGGAGTCATCAGGTATTGTTGGACCTGATCCACCAAGGCATGGTGATCGTGTGGCGAGATCAGCTGCACATGCAACGTCATCACCGGCTGCTCGCCTACCAGCCATACATGTACATGGTGCACATTGCg                                     <  2:426998/142‑1 (MQ=255)
                                                                                                        tCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGAc                                  <  2:242042/143‑1 (MQ=255)
                                                                                                            tCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGAGCGTGTGGCGAGCTCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACCCGGTGCACAGTGCGCACTTcc                              >  1:570640/1‑143 (MQ=255)
                                                                                                               gATAGTGATCCATCAGGTAGTGTTGGATCTGAGCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGAGCACATTGCGGACTTCCggg                           >  2:841372/1‑143 (MQ=255)
                                                                                                                attgTGATCCATGCTCTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCCTCCATACATGTACATGGTGCACATTGCGGACTTCCGGGa                          <  2:134880/140‑1 (MQ=255)
                                                                                                                 tAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGAt                         <  2:55236/143‑1 (MQ=255)
                                                                                                                    tGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACCTGCTGCCCATTGCGCCCTTCCGGGATTTc                      >  2:87131/1‑143 (MQ=255)
                                                                                                                        gggtctggTAGTGTTGGATCTGACCCAACTATGCATCGTGATCGTGTGGCGGGAGCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCGACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCgg                  <  2:1066770/137‑1 (MQ=255)
                                                                                                                          aTCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGca                >  1:759627/1‑143 (MQ=255)
                                                                                                                                  ttgtTGGATCAGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCCTCCATACATGTACATGGTGCACATTGCGGCCTTCCGGGCTTTCCCGGCACCTacgac        >  1:242360/2‑143 (MQ=255)
                                                                                                                                  ttgtTGGATCAGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGCCTTCCGGGATTTCCCGCCACATacgac        >  2:79964/2‑143 (MQ=255)
                                                                                                                                     ttGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCGGGAGCATACGACGcg     >  1:39724/1‑142 (MQ=255)
                                                                                                                                      tGGTTCTGTTCCAACGAGGCTTCCTGATCTTGTGGCCGGATCCCCTGCACATGCAACGTCATCACCGGCTTCTCTCCGCCCATCCGTACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCtt    <  2:59129/143‑1 (MQ=255)
                                                                                                                                       ggATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCttc   >  1:736740/1‑143 (MQ=255)
                                                                                                                                        gATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGCCTTCCGGGATTTCCCGGCACATCCGACGCttca  >  2:781692/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCA  >  NC_000913/783872‑784150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑