Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 916,968 C→T 10.6% R166C (CGC→TGC)  ybjD → DUF2813 domain‑containing protein YbjD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913916,9680CT10.6% 108.3 / 3.4 45R166C (CGC→TGC) ybjDDUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base T (2/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAA  >  NC_000913/916829‑917106
                                                                                                                                           |                                                                                                                                          
ggAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGAGCAGGCACGCCATCTGATGCGTTACATTCCGGTGCTGCGCTTGAGTGATGCACGTTTTAGGCGcc                                                                                                                                         >  2:891324/1‑143 (MQ=255)
     aCGGCAGCGTGATGACACTGCGCAGTGTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTACCGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTAtt                                                                                                                                    <  1:10416/143‑1 (MQ=255)
         cAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATGCGTa                                                                                                                                >  1:3806/1‑143 (MQ=255)
                                ttCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCt                                                                                                         >  1:741084/1‑143 (MQ=255)
                                         aagaagGACAGACGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGaa                                                                                                <  1:240893/143‑1 (MQ=255)
                                         aaGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGaa                                                                                                <  2:478354/143‑1 (MQ=255)
                                              cgACCTCCGATTGATGTCGAGGATATTAACGACCACGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCAc                                                                                           <  1:37110/142‑1 (MQ=255)
                                                aCATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCCAATGTCCCTAATTTGGACGTCCCcg                                                                                         >  2:32276/1‑143 (MQ=255)
                                                aCATCCGATTGATGTCGAGGATATTAAAGATCAGGCACGCCATCTGGTGCGTTTAATGCAGGTGCTGCGATTGCGTGATGCCCGTTTTATGCGCCGGATTCGTCACGGCACGGTGGCAAATGTGCCTAGTGTGGAAGTCAcca                                                                                         >  1:158103/1‑142 (MQ=255)
                                                   tCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTCTTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGTAAGTCACcgcac                                                                                      >  2:60536/1‑141 (MQ=255)
                                                     tgATTGATGTCGAGGATATTAGCGGTCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCgcgcgc                                                                                    <  2:943149/142‑1 (MQ=255)
                                                           aTGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTc                                                                              <  1:1103741/143‑1 (MQ=255)
                                                              tCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGTGCCGTATTCGTCACAACACACAGCGAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGAt                                                                           >  1:466097/1‑143 (MQ=255)
                                                              tCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGTGCCGTATTCGTAACGGCACGGTGCCGAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGAt                                                                           >  1:1038657/1‑143 (MQ=255)
                                                                       ttAGCGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGcc                                                                  <  2:913441/143‑1 (MQ=255)
                                                                        tAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAACTGTCCCTCATGTGGACGTCACCGCGCGCCAGCTCGATCTCCTCcccc                                                                 >  1:119577/1‑143 (MQ=255)
                                                                        tAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGccc                                                                 <  1:353545/143‑1 (MQ=255)
                                                                         aaCGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCg                                                                >  2:106895/1‑143 (MQ=255)
                                                                                aGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTCTTCGTAACGGGCCGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCCGCTCGATTTCCTCGCCCGTGAGTTa                                                         >  1:254244/1‑143 (MQ=255)
                                                                                aGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCACATGTCCCTAATGTGGAAGTCACCGCGCGCCACCTCGACTTCCCCGCCCGGGAGTTa                                                         >  2:898903/1‑143 (MQ=255)
                                                                                aGGCACGCCATCTGGTGCGTTTAAGCCCGGTGCTGCGCTTGCGTGACGCCCGTTTTATGTGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCTTCGCCCGTGAGTTa                                                         <  2:545197/143‑1 (MQ=255)
                                                                                  gCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCGCCTGAGTTATc                                                       >  2:687656/1‑143 (MQ=255)
                                                                                  gCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATc                                                       <  1:430280/143‑1 (MQ=255)
                                                                                       ccATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTGTCCTcac                                                  >  1:273035/1‑143 (MQ=255)
                                                                                         aTCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACAt                                                <  1:589289/143‑1 (MQ=255)
                                                                                                  gTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAAt                                       <  1:390177/143‑1 (MQ=255)
                                                                                                  gTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGCTACCCTCACATCCGCaaaac                                       >  1:241382/1‑142 (MQ=255)
                                                                                                   tttAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCCAAAtc                                      >  1:757919/1‑143 (MQ=255)
                                                                                                   tttAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAAtc                                      >  1:1021830/1‑143 (MQ=255)
                                                                                                    tccaTGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAAtct                                     <  1:612523/140‑1 (MQ=255)
                                                                                                      aaTGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCACCGTATTCGTAACGGCACGATGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCCCCACCTCTTCCACCTCTACTTACCCTCACATCCACAAAAtccca                                   >  2:829048/1‑140 (MQ=255)
                                                                                                        tGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATctctct                                 <  1:165616/143‑1 (MQ=255)
                                                                                                          ccGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAAGCTCTCTGa                               >  1:935481/1‑143 (MQ=255)
                                                                                                            ggTGCTGCGCTTCCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGCGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATg                             <  2:164131/143‑1 (MQ=255)
                                                                                                             gTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATgg                            >  1:34969/1‑143 (MQ=255)
                                                                                                              tgctgcGCTTGCGTGATGCCCGTTTTATGTGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATggg                           <  2:466097/143‑1 (MQ=255)
                                                                                                              tgctgcGCTTGCGTGATGCCCGTTTTATGTGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATggg                           <  2:1038657/143‑1 (MQ=255)
                                                                                                               cgtgtgcTTGCGTGATGTCCTTTTTAGGCGCCGTCTCCGTACCGCCACGGTGCCAAATGTCCCTAATGTGGAAGGCACTGCCCCCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCTGCAAAATCTCTCTGATGTGc                          <  2:379419/138‑1 (MQ=255)
                                                                                                                 tgcGCTTGCGCGTTGCCCGTTTTATTCGCCGTATTCGTAACGGCACGGTGCCAAAGGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAg                        <  2:61202/143‑1 (MQ=255)
                                                                                                                    gcTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGAtt                     >  2:770874/1‑143 (MQ=255)
                                                                                                                     cTTGCGTCGCGCGCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTc                    <  2:1048813/143‑1 (MQ=255)
                                                                                                                       tGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGt                  <  1:859850/143‑1 (MQ=255)
                                                                                                                         cGTGAGTCCCGGTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCa                <  2:14432/143‑1 (MQ=255)
                                                                                                                                    ttttATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCCCATCCGCACAATCTCCCTGATGGGCAGATTCGTAAGGGGCTTTCCg     >  1:204419/1‑143 (MQ=255)
                                                                                                                                       tATGCTCCGGATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCaa  <  2:637340/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGAAGACGGCAGCGTGATGACACTGCGCAGTTTTCTCGATAAAGACGGACATCCGATTGATGTCGAGGATATTAACGATCAGGCACGCCATCTGGTGCGTTTAATGCCGGTGCTGCGCTTGCGTGATGCCCGTTTTATGCGCCGTATTCGTAACGGCACGGTGCCAAATGTCCCTAATGTGGAAGTCACCGCGCGCCAGCTCGATTTCCTCGCCCGTGAGTTATCCTCACATCCGCAAAATCTCTCTGATGGGCAGATTCGTCAGGGACTTTCCGCAA  >  NC_000913/916829‑917106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑