| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,224,242 | A→T | 6.2% | intergenic (+335/+37) | ycgI → / ← minE | protein YcgI/Z‑ring positioning protein MinE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,224,242 | 0 | A | T | 6.2% | 162.1 / 2.1 | 61 | intergenic (+335/+37) | ycgI/minE | protein YcgI/Z‑ring positioning protein MinE |
| Reads supporting (aligned to +/- strand): ref base A (27/28); new base T (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01 | |||||||||||
ATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTG > NC_000913/1224102‑1224383 | aTACACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAct < 1:905481/143‑1 (MQ=255) cATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc < 1:383231/143‑1 (MQ=255) tgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGag < 1:414352/143‑2 (MQ=255) ttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTATGCGTTGAAGa > 2:263489/1‑143 (MQ=255) atttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGAGGAAATAGCAAAGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGCAGa > 1:496277/2‑143 (MQ=255) tgggTGGCAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt < 2:683675/140‑1 (MQ=255) tGGGAACAGCCTTAAGGGGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGGATTCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcgc < 2:665376/143‑1 (MQ=255) gAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt < 2:502966/143‑1 (MQ=255) aCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACCACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa < 2:834914/143‑1 (MQ=255) ccTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAGAAATGCGCTTTTACAGc > 2:742315/1‑143 (MQ=255) cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg > 2:7744/1‑143 (MQ=255) cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg > 1:641874/1‑143 (MQ=255) ttAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg > 1:894260/1‑143 (MQ=255) tAAGGTGTACAGGGGGAGGTGGAAATAGCGATGAGGAGTATCAGCACGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg < 2:225701/143‑1 (MQ=255) tAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg < 1:73363/143‑1 (MQ=255) tAAGGTGTAAAGGGGGACGTGGCAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg < 1:342992/143‑1 (MQ=255) aacagcgAGGTGGTCATAGCAATGAGGAGTATCAGCAAGAATACGCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATACCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa < 2:589191/137‑1 (MQ=255) aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa < 1:823906/143‑1 (MQ=255) aGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc < 2:923579/143‑1 (MQ=255) ggTGGAAATAGCGATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttct < 1:475807/143‑1 (MQ=255) gttgAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg < 2:927166/140‑1 (MQ=255) tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc > 1:242979/1‑143 (MQ=255) aaaTAGCAATTAGGAGTATCAGCAAGATTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc < 1:382109/143‑1 (MQ=255) atatCAAGGAGGAGTATGAGCAAGCATTCTCCCGACTTGCGCACAACTTGGATGAGAGGGATGAAAACCTCAAGGCAGAGATAACTCTGCCTTGAAGGTAAATGCGCTCTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg < 2:394887/143‑1 (MQ=255) aGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGCGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTAGAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt > 1:37213/1‑143 (MQ=255) ccaggaggagTATCGGCAAGAATACTCGCCGCTTCACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa < 1:662168/139‑1 (MQ=255) cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCGTGAAGATGAATGCGCTTTTACAGCGGGCTTATTTCAGTTCTTCTGCttacagaaa > 1:38455/1‑136 (MQ=255) tGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGt > 1:122881/1‑143 (MQ=255) gaggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc < 1:199269/143‑1 (MQ=255) ccAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTACCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg < 1:989159/142‑1 (MQ=255) aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTATGCCTTGTAGATAAATGCGCTTTTACAGCGGGCTTCTTTCAGCTCTTCTGCTTCCGGTAAGGTCCCGTTCAGCt > 1:695774/1‑143 (MQ=255) gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCGTCCGGTAAGGTCACGTTCGGCTc > 1:285059/1‑143 (MQ=255) gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTACTTCCGGGAAGGTAGCGGTCAGCTc > 1:104721/1‑143 (MQ=255) gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa > 2:138787/1‑143 (MQ=255) aTACTCGCCGCTTTACCACAACGTGGATGGGAGGGATGAAAGACTCAAGGCAGAGATAACTCTGCCTTGAAGAGAAATGCGCTTTTACAGCGGGCTCATTTCAGCTCTTCTGCTGCCGTTCAGGTCACGTGCAGCTCGAGaac > 2:26076/1‑142 (MQ=255) tACTCGCCGCTTTACCACAACGTGTATGAGAGGGATGAAAAACTCAAGGCAGAGATAGCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa < 2:909702/143‑1 (MQ=255) tACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa < 2:595054/143‑1 (MQ=255) gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCATCTCAAGCATAGAAat > 1:438217/1‑143 (MQ=255) gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCACGGATAGaaag > 1:730963/1‑142 (MQ=255) gcTTTACCGAAGCGTCGATGCGAGGGATGAAAAACTCAGGGCAGAGATAAGTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGTTTAGTTCAGCTCTTCTGCTTCCTGTAAGGTCACGTTTAGCTTAAGAATAGAAATATc < 2:932318/143‑1 (MQ=255) cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCg > 2:397747/1‑143 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGcc < 1:946907/143‑1 (MQ=255) ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATc > 1:742550/1‑143 (MQ=255) caACGTGGATGAGAGGGATGAAAACCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCttt < 2:407986/143‑1 (MQ=255) cGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGATCTTCTGCTCACGGTAAGCTCACGTTCAGCACAAGAATAGAACAATCGCCctctacctt > 2:837108/1‑138 (MQ=255) ggATGAGAGGGATGAACACCTCAAGGCAGAGATAACTCTGCTTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc < 2:888898/143‑1 (MQ=255) tGAGAGGGATGAAGCACGCAAGGCAGAGATAACTCTGCCTTGAAGATAAACGCGCTTTTACAGCATCTTTGCTTCTGTTTGTGTGGGCCCCACAAGGTCACGTTCAGCTCAAGATTAGGATTATCGCCATCTTTTTGCTCAAg < 2:1077129/143‑1 (MQ=255) tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAg < 1:93358/143‑1 (MQ=255) gagaGGGATGAAAAACTCAAGGAAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGGCACGTTCCGCTCAAGAATAGAAATATTGCCATCTTTTTGCTCAAGc > 1:366599/1‑143 (MQ=255) agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGCAATATCGCCATCTTTTTGCTCAAGCTGt > 1:37418/1‑143 (MQ=255) agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGt > 2:594942/1‑143 (MQ=255) gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAACTATCGCCATCTTTTTGCTCAAGCTGTTCGGtt > 1:914092/1‑143 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCACGCATAGAAATATCGCCATCTTTTTGCTCAAGCTGTAGGGCTa > 2:358181/1‑143 (MQ=255) acaaCTCAAGGCAGTGAGAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAc < 1:821884/141‑1 (MQ=255) aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCCGCTCTTCTGCTTCCGGTAAGGTCACTTTAAGCTCAAAAATAAAAATATCACCATATTTTTATTCAAGTTGTACGGTTAc > 1:92014/1‑143 (MQ=255) aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAc < 2:574988/143‑1 (MQ=255) ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGACATATCGCCATCTTTTTGCTCAAGCTGTAGGGTGACCAtc > 2:818548/2‑143 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTCTTTGCTCAAGCTGTACGGTTACCATCTCa > 2:260150/1‑143 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCACCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCa < 2:200983/143‑1 (MQ=255) aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAg > 1:44647/1‑143 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt < 1:7744/143‑1 (MQ=255) aaCTCTGCCTTGTAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTCTTTGCTCAAGCTGTACGGTTCCCATCTCAGGATCAATTTg > 2:90050/1‑143 (MQ=255) | ATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTG > NC_000913/1224102‑1224383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |