Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,260,904 A→G 7.9% intergenic (‑101/+24) dauA ← / ← prs aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,260,9040AG 7.9% 126.0 / 2.7 51intergenic (‑101/+24)dauA/prsaerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base G (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-01

CGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCA  >  NC_000913/1260766‑1261039
                                                                                                                                          |                                                                                                                                       
cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                     <  1:737933/143‑1 (MQ=255)
ccgAAAGGCATCACATGTGAGGAAAATATTTTGCTCACACGAATGTCTCAGCCGTGAGCGCCTCATGCGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                     <  2:533282/141‑1 (MQ=255)
   aaaGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTc                                                                                                                                  >  1:586990/1‑143 (MQ=255)
    aaGGCACCACATGGGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCt                                                                                                                                 <  1:191596/143‑1 (MQ=255)
      ggCATCCCATGTGAGGAAAATATTTTGTTCCCAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGTtttt                                                                                                                               <  1:554034/143‑1 (MQ=255)
         aTCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAg                                                                                                                            >  1:783814/1‑143 (MQ=255)
            gcaggggAGGAAAATATTTTGTTCACAATAATGTTTCATCCGGGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCg                                                                                                                         <  1:767842/137‑1 (MQ=255)
            acaTGTAATGAAAATATTTTGTTCACAATAATGGGTCATGCGCGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGTGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGTttttttgtct                                                                                                                         <  2:531985/143‑7 (MQ=255)
                    ggAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt                                                                                                                 >  1:709553/1‑143 (MQ=255)
                    gcaaaaTATTTTGCTCACAATAATGCTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt                                                                                                                 <  2:936342/141‑1 (MQ=255)
                     gAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCACCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                                                <  1:678701/143‑1 (MQ=255)
                     gAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGCGCCGGGCTCGact                                                                                                                >  2:325244/1‑141 (MQ=255)
                      aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                                               >  2:336148/1‑143 (MQ=255)
                      aaaaTATTTGGTTCACACGAAGGTTTCAGCCGTGAGCGTATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATTGATATGACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                                               <  2:416953/143‑1 (MQ=255)
                          tatTTTGGTCACAGGAATGTTTCATCCGTGAGCGCATCGTATGTCAACTAACTGGCAGGTGAAGGAGGCATAGGTCATACAAGTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtgt                                                                                                           <  2:429378/143‑1 (MQ=255)
                                     caATAATGTTTCATCCGTGAGCGCATCGTCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCGGCCGGAGCGGGCCTTTGAGCCGGGTTGGATTAGTGTTCGAACCTGGc                                                                                                >  2:854949/1‑143 (MQ=255)
                                      aataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGGCTTTGAGCCGGGTTCGAGTAGTGTTCGAACATGGCa                                                                                               >  1:999163/1‑143 (MQ=255)
                                      aataatGTTTCATCCGTGAGCGCATAATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATCCAAATGAATATTACAGACAAAAAAACGCGCCGCAGCGGGTCTGTGAGCCGGGTTCGATTAGAGTTCGAACATGGCa                                                                                               >  2:360602/1‑143 (MQ=255)
                                                  tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAgtct                                                                                   >  2:719638/1‑143 (MQ=255)
                                                    cGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGCttcttc                                                                                 >  1:846997/1‑143 (MQ=255)
                                                     gTGAGCGCAGCATCTGTCAACTAAATGGCCGATGAAGGTGTCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCg                                                                                <  1:857944/143‑1 (MQ=255)
                                                      tggtcgcACCATCTGTCAACTAAAGGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGt                                                                               <  1:370277/139‑1 (MQ=255)
                                                             atcatcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGGTGAt                                                                        >  1:683888/1‑143 (MQ=255)
                                                                 tcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga                                                                    <  2:86241/143‑1 (MQ=255)
                                                                  cTGTCAACTAAATGGCAGGTGATGGAGGCATAGGTCATACAAATGGATATGACAGACAAAAAAACTCGCCGCAGCCAGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgac                                                                   <  2:835937/143‑1 (MQ=255)
                                                                     gcAACTTACTGGCAGGGGAAGGAGGCATAGGTAATACAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGaa                                                                <  1:796017/142‑1 (MQ=255)
                                                                       aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACACATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCCGAGATCGATTCTTCGTTGCTGCTACGACGAATc                                                              >  2:838664/1‑143 (MQ=255)
                                                                          tAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGCATAATACAGACAAAAGAACCCGCCGCAGCGGGTCTTTTAGCCGGGTTCGATTAGTGTTCGAACACAGCACAAATCGAATCTGCGTTACTGAGaagacgcaacggt                                                           >  2:608702/1‑136 (MQ=255)
                                                                                gCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGccc                                                     >  1:644835/1‑143 (MQ=255)
                                                                                        aggaggCATAGGTTATACATATGGATATTCCTTATCTAAGATCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAcc                                             <  2:777740/143‑1 (MQ=255)
                                                                                         ggaggCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                                            >  1:952761/1‑143 (MQ=255)
                                                                                               aTAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAgg                                      <  1:195980/143‑1 (MQ=255)
                                                                                                 aGGTCATACAAATGGATATTACCGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGTGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                                    <  2:88908/143‑1 (MQ=255)
                                                                                                   gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCCTACCTGCCAGGGTcc                                  >  2:446668/1‑142 (MQ=255)
                                                                                                   gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                                  >  1:913142/1‑143 (MQ=255)
                                                                                                   gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGAGACGACGAATCGCTGCGGCCAGCATACCTGAGAGGGTCa                                  >  1:497377/1‑143 (MQ=255)
                                                                                                     cATACAAATGGTTATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCaga                                <  2:353399/143‑1 (MQ=255)
                                                                                                     cATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCaga                                <  1:336148/143‑1 (MQ=255)
                                                                                                     cATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCaga                                <  1:1106378/143‑1 (MQ=255)
                                                                                                          aaaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCGTACCTGACAGGGTCAGAGTACg                           >  1:279224/1‑143 (MQ=255)
                                                                                                                   ttACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCCTACCTGACCGGGTCAGCGTACGCACGTTCgc                  >  2:251617/1‑142 (MQ=255)
                                                                                                                   gtACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCgg                  <  2:127598/142‑1 (MQ=255)
                                                                                                                           aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt          >  1:615799/1‑143 (MQ=255)
                                                                                                                           aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGcgatt          >  2:753156/1‑143 (MQ=255)
                                                                                                                            aaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgattt         >  2:64353/1‑143 (MQ=255)
                                                                                                                             aaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGAGCGATTAGTGTTAGAACATGGCAGAGATCGGTTCTGCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACCGGGTCAGAGTCCGCACGGTCGGCAGtgatttg        >  1:425384/1‑143 (MQ=255)
                                                                                                                               agaCCCGCTGCGGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgct      >  1:1017717/3‑141 (MQ=255)
                                                                                                                               acaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgat      <  1:446668/141‑1 (MQ=255)
                                                                                                                                  cccGCCGCAGCGAGTCTTTGAGCCGGGTACGATTAGTGTGCGAACATGGCAGAGATCGAGTCTTCGTTGCTGATACGACGAAGCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc   <  1:355355/143‑1 (MQ=255)
                                                                                                                                   ccgccgCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCGGTGATTTGATTTCa  >  1:457196/1‑143 (MQ=255)
                                                                                                                                   ccgccgCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTCCGAACATGGCAGAGATCGATTCTTCGTTGGTGGTACGACGAAGCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa  <  2:537043/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCA  >  NC_000913/1260766‑1261039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑