| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,273,229 | A→T | 10.5% | intergenic (+27/+17) | chaC → / ← ychN | glutathione‑specific gamma‑glutamylcyclotransferase/DsrE/F sulfur relay family protein YchN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,273,229 | 0 | A | T | 10.5% | 148.5 / 8.8 | 57 | intergenic (+27/+17) | chaC/ychN | glutathione‑specific gamma‑glutamylcyclotransferase/DsrE/F sulfur relay family protein YchN |
| Reads supporting (aligned to +/- strand): ref base A (21/29); new base T (2/4); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
AGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAA > NC_000913/1273087‑1273371 | aGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCa > 2:843290/1‑143 (MQ=255) cTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGAGTCGCCTGAGTAAACGTCCCACATACT‑GCGGCGTCCGACGc > 2:853668/1‑143 (MQ=255) attaaaCTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGcccc < 1:637132/140‑1 (MQ=255) tCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTCCTGCTTGGTCGGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCTGACGcccca < 1:805782/143‑2 (MQ=255) tCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGccccc > 1:378568/1‑143 (MQ=255) tCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGAGCAATGGCTGCTGGCGGAGAATTTGCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCTGACGcccca < 1:804608/143‑2 (MQ=255) cAAACTGGGAATGCAGGACGATGTGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCt < 1:320418/143‑1 (MQ=255) agccTGGGAATGCAGGGCGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGACGTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCTGACGccccact < 2:668827/140‑4 (MQ=255) aaaCTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCTGACGccccact < 1:313727/143‑4 (MQ=255) aaCTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTCCTGGCGAAGAATTTTCCGGATGGTGTGTTACATCCGGACTTCGCCTGAGTAAACTTCCCCCATAGC‑GGGCCGCCAGACGCCCCCTCa > 2:315038/1‑143 (MQ=255) cTGGGAATGCAGGACGATGCGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCaaa < 1:882933/143‑1 (MQ=255) ggAATGCAGGACGATGGGCGGAAGGATTTGCTGGGATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACAt < 2:1043278/143‑1 (MQ=255) aaaTGCAGGACGATGGGCTTAATGATTTGCACGTATCGGTAAAAAAACTGTAGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAATACACAGCCCGGATAGT‑GGGGCGTCAGACGCCCCCTCAAACAtt < 2:393230/142‑1 (MQ=255) aTGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTaa < 1:934714/143‑1 (MQ=255) tGCAGGACGATGGGCTGAATGATGTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTaaa < 1:444124/143‑1 (MQ=255) gaggaggGTGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAAt < 1:493192/142‑1 (MQ=255) aCGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTAAGCCCCGAAGTCGCCTGAGTAAACGTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGa < 1:697452/143‑1 (MQ=255) aCGATGGGCCGAATGATTTGCTGGTACCGGTAAAAACACTGCCGGCGGAGAATTTTCCGGATGGGGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGa < 1:299610/143‑1 (MQ=255) cGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAg < 1:240960/143‑1 (MQ=255) tGAATGATGTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTAt < 2:680406/143‑1 (MQ=255) aaTGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCg > 1:120793/1‑143 (MQ=255) tGATTTGCTGGGATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGc < 2:722380/143‑1 (MQ=255) gATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCTGCGTAGA‑AGCGCGCCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCt > 1:252103/1‑143 (MQ=255) agttGCTGGTAGCGGTAAAAAAACCGCTGGCGGAGAAGTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTg < 2:434941/141‑1 (MQ=255) aTTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTg < 1:1035911/143‑1 (MQ=255) tGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGACAGACGCCCCCTCAAACATTAAAATGTGAGCACTGTATCGGCTGACa > 1:859217/1‑143 (MQ=255) gatGGTATCGGAGAAAAAGCTGCTGGCGGAGAGTTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATACGCTGAccg < 2:193282/141‑3 (MQ=255) tGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGGCAGACGCCCCCTCAAGCGTTAAGATGTGAGGCCGTTGTCGGCTGACAGCg > 1:739392/1‑143 (MQ=255) ggggagcGGCAAAAAGACTGCTGGCGGGGAATTCTCCGGATGGGGTGGTACGTCCGCGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCg < 1:9956/137‑1 (MQ=255) gTATCGATAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCAGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GCGGCGTCAGACGCCCCCCCAAACCTTAAAATGTGAGCACTTTATCGGCTGACAGccac > 2:344753/1‑140 (MQ=255) gTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTgc > 1:820750/1‑143 (MQ=255) aaaaCTGCTGGCGGGGAATTTTCCGGATGGTGTGTGACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACCTTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAg < 2:996876/143‑1 (MQ=255) aaCTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGtt < 2:45340/143‑1 (MQ=255) tgGCAGAGAATGTTGCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACaa < 2:519275/143‑1 (MQ=255) gCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAaga < 2:1006487/143‑1 (MQ=255) gagaATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTa < 1:610416/143‑1 (MQ=255) tttCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCGGTTCCGCGAGAGTACCGAtt > 1:419270/1‑143 (MQ=255) gtgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACCGCGTCCATTCCGCCCGTTCCACAAGAGTACCGATTTCCCCCccacca > 2:148811/1‑143 (MQ=255) tgtTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAACGTCCATTGCGCCAATTCCACAAGAGTACCAAATTCCACCCCatcaa > 2:146150/1‑143 (MQ=255) ttACGTGCGTGATCCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaatc < 2:378568/143‑1 (MQ=255) aCGGCCGGGATGCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCag < 2:236878/143‑1 (MQ=255) tgtggggTTTGCCTGAGTAAACTTTCCGCCTCGT‑GGGGTGTCTGCCGCCCACTCAGACATTGAAATGTGGGCGGTTTATCGGCTGACAGCGTCCATTGCGGCTGTTCCGCTCGAGTGCCGGTTTTCACCGCGTCGATCagagt > 2:241686/4‑142 (MQ=255) ccTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCTTCCATTGCGCCCGTTCGCCAAGAGTACACTTTTGAACCCCGTTGATCAGAGCAGGTGTACtcg > 1:151062/1‑141 (MQ=255) cTGAGTAAACTTCCCGCATAGTGGGGGCGTCAGACGCCCCCTCAAACATTAAACTGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGCTTTCCACCCCATCCATCGGAGGAAGTGTACTaa > 2:347816/1‑143 (MQ=255) tGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATc > 2:181992/1‑143 (MQ=255) gTAAACTTCCCGCATAGT‑GGGGCGTCAGACGACCAACCTAACATGAATATGTGAGCACTTTATCGGCTGACAGCGTCCATGGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCcg < 2:229995/143‑1 (MQ=255) tAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCACCAATCAGAGGAAGTGTACTAATCCcgc > 2:505265/1‑143 (MQ=255) tAAACTACGCGCAGAGT‑GGGGCGGCAGACGCCCTCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCcgc < 2:484757/143‑1 (MQ=255) aaCTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCgcgc < 2:146697/143‑1 (MQ=255) cTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGAGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGccc > 1:807476/1‑143 (MQ=255) ttCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACCGTATCGGCTGACAGCGTCCATCGCGCCAGTTCCACCAGAGTAGCGGCTTCCACCGCATCAATCAGAGGAAGTGGACTAATCCCGCACCCg > 2:999432/1‑143 (MQ=255) ccGCATTGT‑GGCGCGTCAGGCGCCCCCTCAAACCGTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCACTGCGCCAGTACCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCg < 2:423165/143‑1 (MQ=255) cGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAGAATGTGAGCACCTTATCGGCTGACAGCGTCCACTGCGCCAGTTCCACAAGAGTACCGATTTCCCCCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCgg < 2:964259/143‑1 (MQ=255) cGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCgg > 1:48467/1‑143 (MQ=255) gCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCCGTTCCACCCCAGTACCGCTTTCCACCCCCTCGCTCCCAGGCAGTGTACTAATGCCGCGCccgtccgt > 1:69437/1‑143 (MQ=255) agggggCGTCTGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAgg > 1:578407/3‑143 (MQ=255) ggggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGCCAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCCCCCCAACACTCAGCGGCAGTGTACTAAGCCCGCGCCCGACGGTACAGGtc > 2:501883/1‑142 (MQ=255) gggCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGCCGGTACCgggct > 2:809018/1‑140 (MQ=255) aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACaa < 1:1074739/143‑1 (MQ=255) aGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATGGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACaa < 1:109634/143‑1 (MQ=255) | AGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGT‑GGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAA > NC_000913/1273087‑1273371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |