Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,330,399 A→T 6.7% intergenic (+18/+18) sohB → / ← yciN S49 peptidase family protein/protein YciN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,3990AT 6.7% 171.7 / 4.8 62intergenic (+18/+18)sohB/yciNS49 peptidase family protein/protein YciN
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base T (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-02

GTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAC  >  NC_000913/1330259‑1330539
                                                                                                                                             |                                                                                                                                            
gTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATGGATGTAAAAGACAAACGCGAGG‑CTAAg                                                                                                                                            >  2:106877/1‑143 (MQ=255)
           atgCTTTATTCATCGTTATCGTCTCATTGACCGATTAGACGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt                                                                                                                                 <  1:115396/141‑1 (MQ=255)
           aCGCTTGATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt                                                                                                                                 <  2:1044730/143‑1 (MQ=255)
              cTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGAGTGTTGCTACGCTGGTGGCAGCGGGGTCCAAAGCCATTGACGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGc                                                                                                                              >  2:182862/1‑143 (MQ=255)
              cTAGATGCAGGGTAAACGGCGCGTTGACCGATTCACCGGCAGAGGGAATCCGCAGTCCTATCGGTTGTGGCGCCGCGGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGc                                                                                                                              <  2:492412/143‑1 (MQ=255)
                atatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCtt                                                                                                                            <  1:766135/143‑1 (MQ=255)
                atatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTATTGAtt                                                                                                                            >  1:848634/1‑143 (MQ=255)
                  atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTa                                                                                                                          <  1:183172/143‑1 (MQ=255)
                    gCAGCGTGCACGACTCCTTGACCGCTTCGTCGGCAGCGCGGCACAGCGCGCCGATCCATTGTTGGTCCGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTACACGACCAACGCGCCG‑CGGAGACCTCGCGTTTTGCTTTAAt                                                                                                                        <  2:966639/143‑1 (MQ=255)
                        cGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                    >  1:787126/1‑143 (MQ=255)
                           aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATAGATGTAAAAGACAAACGCCAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACAAg                                                                                                                 >  2:689750/1‑143 (MQ=255)
                            aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAga                                                                                                                <  1:110539/143‑1 (MQ=255)
                            aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCCCGCGTTTTGCTTTAATCAACCAga                                                                                                                <  1:353813/143‑1 (MQ=255)
                             aCGTCTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat                                                                                                               <  2:354896/143‑1 (MQ=255)
                                 cTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata                                                                                                           <  2:1054206/143‑1 (MQ=255)
                                             ttCACCGGCAGCTCTGTATATATCATCAATCGATTTTTGCTACGCTTGTTGCATCGGTGTCAAAATCCATTTATGTAAAATACAAACTCGAGT‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATTATATTTTTCTTAAAg                                                                                               <  2:894529/143‑1 (MQ=255)
                                             ttCACCGGCAGCTCTGCACAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCATCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGATG‑CTAAGACCTCTCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg                                                                                               <  2:510286/143‑1 (MQ=255)
                                              tCACCGGCAGCGCGGCACAGATCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGTTCAAAATCCAGTTATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGc                                                                                              <  2:835285/143‑1 (MQ=255)
                                                aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAACGCCATTGCTGTAGAAGACAAACGCGAGG‑CTAAGCCATCGCGTTTTGCTTTAATCAACCAGCTGATATTTTTCTGCAAGcac                                                                                            >  2:936829/1‑143 (MQ=255)
                                                aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGCTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac                                                                                            <  1:988194/143‑1 (MQ=255)
                                                                agagCGCGGATCGATTGTTGCTAAGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGGGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTg                                                                            <  2:690554/143‑1 (MQ=255)
                                                                     gcCGCTCGATTCTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAACACCTCGCGCTTTGCTTCAATCAACCAGATGACATCTTCCTGAAAGCACATCAGCCACGCGCTTGagcct                                                                       >  2:403393/1‑139 (MQ=255)
                                                                       cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACatat                                                                     >  1:254858/1‑143 (MQ=255)
                                                                         atcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACAGATTa                                                                   >  1:368116/1‑143 (MQ=255)
                                                                            gatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCCGGTGTTTGAACATATTAAac                                                                >  1:976097/1‑143 (MQ=255)
                                                                              ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGCACATATTAAacac                                                              >  2:955993/1‑143 (MQ=255)
                                                                              ttgttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTCAacac                                                              >  1:850131/1‑143 (MQ=255)
                                                                                  tgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAGACACcgcg                                                          >  1:837024/1‑143 (MQ=255)
                                                                                     tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTg                                                       >  1:810252/1‑143 (MQ=255)
                                                                                        gCTGGTGGCAGCGGGGTCAAAAGCCATTGGCATAAAAGACAAACGCGAGG‑AAACGACATAATTATTAGATTTTATCAAGAAGATGATATTACTCTGAAAGCACATGGGCCAGGTGTTGGAACGTATTAAACATCGCGGtaatg                                                    >  2:785610/1‑139 (MQ=255)
                                                                                           ggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACACGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTCTTg                                                 <  2:966675/143‑1 (MQ=255)
                                                                                            gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCATGTGTTTGAACATATTAAACACCGCGGTGCTTTTgg                                                <  1:21041/143‑1 (MQ=255)
                                                                                                 aGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGAGGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGtt                                           <  1:837934/143‑1 (MQ=255)
                                                                                                   cGGGGTCAATAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGGTTTGCTTTAATCAACCAGATGATATTTTTCGGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg                                         <  2:681472/143‑1 (MQ=255)
                                                                                                      ggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCaa                                      <  1:666039/143‑1 (MQ=255)
                                                                                                       gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTCGCACCTATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                     >  2:403377/1‑143 (MQ=255)
                                                                                                          aaaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCACTcac                                  >  2:472698/1‑141 (MQ=255)
                                                                                                           aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGCTATTTTTCTGAGAGTACCTGTGCCAGGTGTTTGACTATATTCAGCACTGCGGTGCTTTTTTCTGTTGTCAAtacct                                 >  2:126996/1‑139 (MQ=255)
                                                                                                             aGCCATTGATGTAAAAGACCAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                               <  1:301496/143‑1 (MQ=255)
                                                                                                               ccATTGATGTAAATCACAAACGCAAGG‑CTAATCCCTCGCGTTTTGCTTTAATCATCCAGATGATATTTTTCTGAAAGCTCATGGGCCAGGTGTTTGAACATATTAAACGCCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                             <  1:954742/143‑1 (MQ=255)
                                                                                                                      tgaaaaaGACGAACGCGCGG‑CTAAGACCTCGCGGTTTGCTTTAATCACCCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaa                      <  1:80749/140‑1 (MQ=255)
                                                                                                                        tacaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                    <  1:726206/140‑1 (MQ=255)
                                                                                                                        tAAACGACAAACGCGCGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                    <  1:797222/143‑1 (MQ=255)
                                                                                                                        tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                    <  2:787126/143‑1 (MQ=255)
                                                                                                                        tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                    >  1:892498/1‑143 (MQ=255)
                                                                                                                        tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAATTTTCAGAAAGCACAAGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                    >  1:1047935/1‑143 (MQ=255)
                                                                                                                          aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt                  >  1:25105/1‑143 (MQ=255)
                                                                                                                          aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt                  >  1:552838/1‑143 (MQ=255)
                                                                                                                           aggACGAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATCTTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                 <  2:537011/141‑1 (MQ=255)
                                                                                                                           aaGACAAACGCGGGG‑CTAAGACCTCGGGTGTTGCTTTAATCAACCAGATGATACTTTTCTGAAAGCCCGTGGGCCAGGTGTTTGATCATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                 <  2:301764/143‑1 (MQ=255)
                                                                                                                            aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTATTCATCTAAAAagtac                >  2:688854/1‑142 (MQ=255)
                                                                                                                            aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCCGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                >  2:689041/1‑143 (MQ=255)
                                                                                                                               aaaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg             >  1:1013871/2‑143 (MQ=255)
                                                                                                                                aaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc            >  1:540206/1‑143 (MQ=255)
                                                                                                                                aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc            >  1:728812/1‑143 (MQ=255)
                                                                                                                                aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc            >  1:818461/1‑143 (MQ=255)
                                                                                                                                aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc            >  1:328457/1‑143 (MQ=255)
                                                                                                                                  aCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg          <  1:112394/143‑1 (MQ=255)
                                                                                                                                   ctcgACG‑CTAAGACCTCGCGTTTTGCGTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTTCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg         <  1:314472/141‑1 (MQ=255)
                                                                                                                                        gg‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGGACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTGAAAAGTAGTCGCCGGTGAAt    >  1:127413/1‑143 (MQ=255)
                                                                                                                                           cTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAc  <  1:675210/143‑1 (MQ=255)
                                                                                                                                           cTAAGACCTCGCGTGTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAc  <  2:406690/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAC  >  NC_000913/1330259‑1330539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑