| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,344,033 | A→G | 10.0% | L105P (CTG→CCG) | yciT ← | putative DNA‑binding transcriptional regulator YciT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,344,033 | 0 | A | G | 10.0% | 123.3 / 5.6 | 56 | L105P (CTG→CCG) | yciT | putative DNA‑binding transcriptional regulator YciT |
| Reads supporting (aligned to +/- strand): ref base A (18/30); new base G (4/2); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01 | |||||||||||
GTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTA > NC_000913/1343893‑1344173 | gtgtCCAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCaga < 2:39302/143‑1 (MQ=255) aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACCCGCCACCGAGCAAAATAACTTCACATGTCTCGTCCTTCCGCAGATGCGCGGCGTAGCTGCTGACCGTCCTGACCGTGACATTTTTCTTCTGCTCGCTCCGTGTCCg > 2:660127/1‑143 (MQ=255) aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTACGCAAATTCGCGATGTAGATGCTGAGCGTGATGATAGTGACATTTTTCTTATGCTCGCCCAGAGTCCg > 1:152221/1‑143 (MQ=255) aaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg > 1:229911/1‑143 (MQ=255) ccAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGCGCAAAATAACTTTACACGGCGCTTCTTTCTGCTCACCCCGGATGTAGCTGCTGACCGTGATGCTGGTGTCCTTTTTCTTCAGATCGCTCTGAGCCggggtcag > 2:26952/1‑139 (MQ=255) ccAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg < 1:160273/143‑1 (MQ=255) cAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc < 1:742697/143‑2 (MQ=255) aCCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg < 1:710009/143‑1 (MQ=255) ccATACTTTCGCTTTTTTGCTGACACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAAGGCGCGATGTAGCTGCTGACCGTGATGATAGTGACCTTTTTCTCATGCTCGCCCAGAGTCCGGGCGAGTAgg < 1:160348/143‑1 (MQ=255) aTACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGCGTAGGGc > 1:524814/1‑143 (MQ=255) tttCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg > 1:547336/1‑143 (MQ=255) tCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt > 1:869148/1‑143 (MQ=255) gATACGCGCCACCGAGCAAAATAACTTCCCAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCg < 1:315606/143‑1 (MQ=255) gacaaGCCACTGAGTAAAATAACTTGACAAGGCGCTTCTTTCAGAAAAGGCGCGATGTAGCTGCTGACCGTGATGAAAGTGACATTTTTCGTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt < 1:532916/142‑1 (MQ=255) acacGCCACCGAGCAAAATAACTTCACCAGGTGAGCCTACCGGCAACGGCGAGATGTAGCCGCTGACTCTGGTGAGCTCGGCATTTTGCGTCTGCTCGCCCAGAGTCGGCGCGCTCGGGGCATTGCTGCTGCCCtttttgcta > 2:963923/1‑138 (MQ=255) acacGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa > 1:219419/1‑143 (MQ=255) acGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa < 1:366543/143‑1 (MQ=255) aCCGAGCACAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATgg < 2:627520/143‑1 (MQ=255) aGCAAAATAACTTCACAGGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc < 2:842343/143‑1 (MQ=255) gCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCg < 1:967269/143‑1 (MQ=255) gCAAAATAACGTGACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGCCCGTGATGATAGTGACATTTTTCTTCTGCTAGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCg < 2:550271/143‑1 (MQ=255) gCAAAATAACCTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCg < 1:354421/143‑1 (MQ=255) taccggcacgAGGCGCGTCTTTCTGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt < 2:1101677/137‑1 (MQ=255) tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt < 1:334490/143‑1 (MQ=255) aaCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt < 2:453961/143‑1 (MQ=255) cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa < 2:257403/143‑1 (MQ=255) ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa < 1:993417/143‑1 (MQ=255) ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa < 1:289989/143‑1 (MQ=255) tCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCCCCCAGAGGCTGGGCGAGGAGGTCAGTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc > 2:491626/1‑143 (MQ=255) ccccaAGGCCCGTCTTCCAGCAAATGCGCGATCCAGCTGCTGACCGTGATGATAGTGACATTTTTCCTCTGCCCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc < 1:261963/140‑1 (MQ=255) cacggCGCGTCGTTCAGCATATGCGCGAGGTAGCTGCTGGCCGTGCTGCTGGCGACAGATTTCTTGTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg < 2:234209/140‑1 (MQ=255) aaGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc > 2:925977/1‑143 (MQ=255) cgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCAATTTTCGATAAAGATGGTTTCGCCAGGTTGAACAAACGAcgcg > 2:94651/1‑143 (MQ=255) cTTTCAGCGAATGCGCGATGTAGCTGCTGCCCGTGATGCTAGTGACATGTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAAGGAATTGCCTCTTTGAACCAGCGACGCGGCa < 2:967874/143‑1 (MQ=255) gCAAATGCGCGATGTAGCTGCTGACCGTGATGATGGTGACATTTTTCTTGTGCTCGCCCAAAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg < 1:923867/143‑1 (MQ=255) gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGTGTCGGGGAGAGAAGAACAGTGCTGCTGCCATTTTCGACAAAGATGGTTTCGCCCGGGTGGACCAGCGACGCGGCAAACTCg > 1:514769/1‑143 (MQ=255) gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTGCGCCCGGGTGAACCAGCGACGCGGCaaagacg > 2:852867/1‑139 (MQ=255) gcccgATGTAGCTGCTGACCGTGATGATAGTGACATGTTTCTTCTGCTCGCCCAGAGTGCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACGCGGCGAGt < 2:515777/140‑1 (MQ=255) gTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAACCTCGGCGAGTTCACGCTTCAGCGTATAGtt > 1:815579/1‑143 (MQ=255) gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGc < 2:219419/143‑1 (MQ=255) gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGCATAGTTGc > 1:169846/1‑143 (MQ=255) tgatGGTGACATGTTTCTGCGGCGCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCACATTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtc < 2:11811/143‑1 (MQ=255) tAGGGAAATTTTCCTCGTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatc < 1:979227/143‑1 (MQ=255) aCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGAATTGCTGCTGTCATTTTCGATAAAGATGGTTTCGCCCAGTTGAACTAGCGACACAGCAAACTCGGCGAATTCACTCTTAAGCGTAAAGTTGCTGataataag > 2:241923/1‑141 (MQ=255) aCATTTTTCTTCTGCTCGCCCACAGCCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTGGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACg < 1:694811/143‑1 (MQ=255) aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTCTAGTTGCTCATCATACggg > 1:772647/1‑143 (MQ=255) tcttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCg < 1:660127/143‑1 (MQ=255) cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCAGTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCGGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGa < 2:37975/143‑1 (MQ=255) ttgttcTCGCCCAGAGTCCGGGCGAGTAGCGCATTGCTGCTGCCATTTTAGATAAAGATGGTTTCGCCCGGTTGCACCAGCGACGCGGCAAACTCGGCGAGGTCACGCTTCAGCGTATAGTTGCTCATCATCCGGGTTTCGAc > 2:738717/6‑143 (MQ=255) cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTGTCGACg > 1:853348/5‑143 (MQ=255) ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGt > 1:644305/4‑143 (MQ=255) tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGCTAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCgtcgtc > 1:928981/3‑143 (MQ=255) tGCTCGCGCGTAGGCCGTGCGAGTAGGGCATTGCTGCTTCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtc < 2:104348/143‑1 (MQ=255) tGCTCGCCCAGGGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtc < 2:832235/143‑1 (MQ=255) cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCAGCTCCCGTTTTCGATAAAGATGGTTTCGCCCGGTTGAACCCGCGACGCGGCAAACTCGGCGAGGTCACGCGTCAGCGTATAGTTGCTCACCCTACGGGTTTCGACggcct > 2:579675/1‑139 (MQ=255) cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAGTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcatca < 1:371497/143‑1 (MQ=255) gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGATCATCATACGGGTTTCGACGTCATCAc > 2:413229/1‑143 (MQ=255) cgagagTCAGTGCGAGTAGGGCATTGCTGCTGCCATCTTCGATGTAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTa < 2:848467/141‑1 (MQ=255) | GTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTA > NC_000913/1343893‑1344173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |