| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,689,821 | T→G | 9.8% | intergenic (+70/‑31) | manA → / → ydgA | mannose‑6‑phosphate isomerase/conserved protein YdgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,689,821 | 0 | T | G | 9.8% | 133.5 / 3.3 | 63 | intergenic (+70/‑31) | manA/ydgA | mannose‑6‑phosphate isomerase/conserved protein YdgA |
| Reads supporting (aligned to +/- strand): ref base T (29/25); new base G (3/4); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
CGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGG > NC_000913/1689685‑1689960 | cgccAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCAGCCt > 1:1040017/1‑143 (MQ=255)cgccAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCt < 2:454981/143‑1 (MQ=255) cAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaa < 1:971187/143‑2 (MQ=255) aaCGAATCACCGTTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGAtt < 2:540219/143‑1 (MQ=255) cGAATCACCTGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTc < 2:995789/143‑1 (MQ=255) cACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCAttta > 2:605271/1‑142 (MQ=255) gTGACTGTCAACGGCCACGGCCTTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCGTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGa < 2:646933/143‑1 (MQ=255) tGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGAtcacc > 2:170607/1‑139 (MQ=255) tCAAAGGCCACGGCCGTTTAGCGCGTGCTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCAt > 1:778256/1‑143 (MQ=255) aaaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTa < 2:40877/143‑1 (MQ=255) aaaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTa > 2:156280/1‑143 (MQ=255) aaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTAt < 1:1049710/143‑1 (MQ=255) aaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCACGTTAGGGATAATCATTAt > 2:717576/1‑143 (MQ=255) cggCCACGGGCGTTTGGCGCGTGGTTACAACTCACTGTATGCTCTTACTGAAAAAATTAACATCTCTTGCTAAGCGTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATg < 2:1069359/142‑1 (MQ=255) cACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGACCACCAAACCTCCCGATTTCATCTTAATGCTAATCATTATGAATa > 2:780323/1‑143 (MQ=255) cGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATCTTATGGATAATCATTATGAATaaa > 2:199060/1‑143 (MQ=255) gCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATc > 1:469801/1‑143 (MQ=255) tttAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTg < 1:343814/143‑1 (MQ=255) tttAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCACGCGGCCAGTCCGCCTGCGTTCATTTCATCCATTATCATTCTAAATAAATcccgg > 2:1011748/1‑139 (MQ=255) cgcgTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGTATAATCATTATGAATAAATCGCTGGTAGc < 1:700633/143‑1 (MQ=255) tgtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCGCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTa < 1:873577/143‑1 (MQ=255) caacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATGACACCTTCAGGCGGCCAGCCCGACTGATTTCATTTGATGGATCATCACTATGAACAAATCGCTGGTAGCGGAAGGCggt > 2:280256/1‑141 (MQ=255) caacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTc > 2:703187/1‑143 (MQ=255) aacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCa > 2:209909/1‑143 (MQ=255) aacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCACTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCa > 2:544659/1‑143 (MQ=255) gccgcaaGAGCTTACTGAAAAAATTAATATCTCTTCCTATGCTTATGAAAGTCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttttt < 2:723751/138‑1 (MQ=255) gCTGTACGGCCTTACTGAAAACATTAACATCTCTTGCTAGGCTTATTAAAGGCTTATAACTCCTTCAGGCGGCCAGTCCGCCTGATTTCATCTTCTGGATCATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgtt < 1:520054/143‑1 (MQ=255) cgttAACAGCTTACTGAAAAAACTAACATCTCTTGCTAATCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgttg < 2:168813/140‑1 (MQ=255) cTGTAAGAGCTTACTGAAAAAAGTAACATCTCTTGCTAAGCTTATTCCGGGCTGATAACACCTTCAGGCGGGCAGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgttg < 1:1047659/143‑1 (MQ=255) tAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgcgc > 1:11005/1‑143 (MQ=255) tAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgcca > 2:554831/1‑141 (MQ=255) nagacCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTACAGGCTTATCACACCTTCAGGCGGCCAGTCCGCCAGCTTTCATTTTCTGGATAATNATGATGTATACAGCGCCGGTACCGGTCGCCGTCATTGTTGGGCt > 2:373920/2‑143 (MQ=255) agagCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCCGCTAGCGGTAGGCGTCATTGTTCCACTa > 2:33419/1‑143 (MQ=255) taacgAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTa < 1:435075/139‑1 (MQ=255) acacCTCTTGCTAAGCTTATTAAAGGCGTATAACTCCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcgg < 1:717286/143‑1 (MQ=255) cATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcggc < 2:904392/143‑1 (MQ=255) ctTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATg < 1:192924/143‑1 (MQ=255) ctTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATg < 1:192992/143‑1 (MQ=255) ctTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATg < 1:680433/143‑1 (MQ=255) gggatgCTTGTTAAGGGGTTATATGGCCTTCAGGCGGCCAGTGCGCCTGATTTCATTTTATGGATAATCATTATGAATAGATGGCTGGTAGCGGTAGGCGGCATTGTTGCGCTAGGCGTAGTCTGGACAGGTGGCGCATGGta < 2:878123/138‑1 (MQ=255) gCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGta > 1:650103/1‑143 (MQ=255) agcgtgttaAAGGGTGAGAACACCCTCAGGCGGCCAGGCCGCCCGGCTGCAGGTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAGTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATAcn < 1:373920/137‑2 (MQ=255) gCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTGGGCTGGACAGGCGGCCCATGGTATagag > 2:270722/1‑143 (MQ=255) attcAAGGCTTATAACTCCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCATCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCaa < 2:570516/143‑1 (MQ=255) taAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGCCAGGCGGCGCATGGTATACGGGCaaga > 1:263484/1‑143 (MQ=255) aaaGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATGCAGGCaagaa > 1:80105/1‑143 (MQ=255) aGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAagaaga > 1:672414/1‑143 (MQ=255) aGGCTTAGATCACCTTCAGGCGGCCAGTCCGCCTGAGTGCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAagaaga < 2:657360/143‑1 (MQ=255) gCTTATAACGCCTTGAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAtt < 1:363797/143‑1 (MQ=255) ttATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTAATGGATAATCATTATGAATAAATGGCAGGTAGCGATAGGCGACATTGATGCGCAAGACGTGGTCTGGACAGGCGGCGGACGGGATACAGGCacgaagctaga > 2:520016/1‑138 (MQ=255) cacCTTCAGGCGGCCCGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTTGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCa < 2:177661/143‑1 (MQ=255) cacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCa > 2:275183/1‑143 (MQ=255) cacCTGCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCAGTAGGAAGCAGTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCa < 1:156280/143‑1 (MQ=255) aaatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGACTGAAGccccgc > 1:426978/4‑140 (MQ=255) cTTCAGGCGGCCAGTCCGCCTGACTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATCGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCacca > 2:318892/1‑140 (MQ=255) aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTAGTACCGTGTTTCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCAtct > 1:416510/3‑143 (MQ=255) ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCAtctc > 2:115063/1‑143 (MQ=255) ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAATGAAACCTAtctc > 1:683547/1‑143 (MQ=255) tCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCg > 1:607351/1‑143 (MQ=255) aGGCGGCCAGTCCGCCTGATGTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGaa < 1:199060/143‑1 (MQ=255) gcggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGa > 1:570130/1‑143 (MQ=255) ggcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATCCATGCAAGAAGTTTGACACCCATCTCGAAGCCa > 1:920516/1‑143 (MQ=255) cAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATgg > 1:637516/1‑143 (MQ=255) | CGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGG > NC_000913/1689685‑1689960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |