| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,778,375 | A→G | 9.0% | intergenic (+28/+15) | ydiO → / ← ydiP | putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,778,375 | 0 | A | G | 9.0% | 128.0 / 3.4 | 55 | intergenic (+28/+15) | ydiO/ydiP | putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP |
| Reads supporting (aligned to +/- strand): ref base A (24/25); new base G (2/3); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01 | |||||||||||
GTCTCCCGCTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAA > NC_000913/1778237‑1778515 | gTCTCCCGCTTCGTGGGGT‑AGGTCCGGTGCGGACGTAGCGCGGGCGGTACAGACGAAATTCTGATTTACGGAGCCGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgc < 2:696095/143‑1 (MQ=255) ctcCCGCTTC‑TGGCGTGGTGTCCGGTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTgcgc < 2:595183/143‑1 (MQ=255) cccGCTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgccg < 1:374783/143‑1 (MQ=255) ccGCTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc > 1:228847/1‑143 (MQ=255) cGCTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgcc > 1:986533/1‑143 (MQ=255) cTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGGAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACACATAATCTGCAGGCGGCGCAGCTGCTTAGCAAGCTGCGCCGCCAg > 1:419942/1‑143 (MQ=255) ttC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGa > 1:453051/1‑143 (MQ=255) c‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGACATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTGCTTAACAGACTGCGCCGCCaacat > 1:595491/1‑139 (MQ=255) gTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGACAGACTATCAGAACAACTATTCTGCCGGCGGCGCAGCTTCTTAACAAACTGCGCCACCAGATTTAtac > 1:319030/1‑141 (MQ=255) tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCGGGCGGCGCAGCTTCTTAACAAAATGACCCCCCAAATCTATcaa > 2:365593/1‑141 (MQ=255) tGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCACCTTCTTAACAAACTGCGCCGCCAGATTTATCca > 1:823520/1‑143 (MQ=255) aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAGGTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac < 2:113417/143‑1 (MQ=255) aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac > 2:801655/1‑143 (MQ=255) aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac < 1:1031072/143‑1 (MQ=255) gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTgcgc < 1:428334/120‑1 (MQ=255) gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTgcgc > 2:428334/1‑120 (MQ=255) gtgAACGTAGCGGCGGCAGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc < 2:291887/143‑1 (MQ=255) gAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTCACCg > 2:842807/1‑143 (MQ=255) aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTCCCgg > 2:205731/1‑143 (MQ=255) aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCgg < 1:144108/143‑1 (MQ=255) aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTCACAAACTGCGCCGCCAGATTTATCCAACAAGACTCAcacga > 2:770558/1‑139 (MQ=255) aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg > 1:341629/1‑143 (MQ=255) cggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTACTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGCAAt > 2:327203/1‑143 (MQ=255) ggcggTACAGACGAAATGATGATCTACGGAGCAGGTCGGCAGACCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACCTACCGGTGGGGGAAAtt < 2:141317/143‑1 (MQ=255) cggTACAGACGAAATTATGCTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCa < 1:529525/143‑1 (MQ=255) cggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGAGGCGCAGCTACTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACGTACCGGTTGAGGCCATTCa > 2:1037648/1‑143 (MQ=255) cgctccAGGCGAAATTAGCTGTTACGTAGGAGGTCGTCAGTTCCGGAAAGTCTAGCTGAACAGATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGTCAGATTGATCCAACTAGACTTACCGGTTGAGGAAATTCa < 2:585182/138‑1 (MQ=255) aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGCGGAAATTCAGATAgg > 2:1033767/1‑143 (MQ=255) aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAt > 1:361364/1‑143 (MQ=255) aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCGACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt > 2:1017975/1‑143 (MQ=255) aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt < 1:734450/143‑1 (MQ=255) aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt < 1:479683/143‑1 (MQ=255) tATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGGGGAAATTCAGATAGGATATCTTGt > 1:1035509/1‑143 (MQ=255) tttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCGTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGc > 2:557370/1‑143 (MQ=255) gcGTAGCAGGCCGGCAGATCCGGAAAGACGACCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACACTCTGCGCCAACAAATTTATCCAACAAGTCTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaaa < 1:42952/142‑1 (MQ=255) agcacttCGGCAGCTCCTGAAATACTCTCAAAACAAATAATCTGCAAGCGCCTCAGCTTCTTAGCAAACTGCACCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAg < 2:348159/137‑1 (MQ=255) gcggTGGGCAGATCCGGAAAGGGTATCTGATCAAGTAGTCTGCAGGCGGCGCAGCTTCTTAACAAACTGGGTCGTTAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt < 2:15043/141‑1 (MQ=255) cAGATCCTGAACGACTATCAGAACAAATACTCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAAtt < 2:869874/143‑1 (MQ=255) aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCCTAACAAACTGCGACGTCAGCTTTATACAACAAGCGCTTTGGATCGATACCGTTCAGATAGGATTTCTTGTTGCGCAAAACAGTTTTTAAATTg > 2:1076655/1‑143 (MQ=255) tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACg < 2:553476/143‑1 (MQ=255) ggAAAGAGTATCAGAACAAATAATCCCCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACGGACCGGTTGAGGAAATGCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACg < 2:883766/142‑1 (MQ=255) aCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATc < 1:706107/143‑1 (MQ=255) aCTATCAGAACAAATAATCCGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATc < 1:361198/143‑1 (MQ=255) tCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg < 1:235380/143‑1 (MQ=255) cAGAACCAATCAGCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACCAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTgg < 2:37805/143‑1 (MQ=255) aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCGACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTggg < 1:1017975/143‑1 (MQ=255) aaCAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTCCCGAAAAACAGTTTTTAAATTGTCTACGGTAATTGCTGGGCg < 1:471210/143‑1 (MQ=255) cgagcctCTGCCGGCGGCGCAGCTTCTTAACAAACTGCGCCGTCAGATTTATCCAACATGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTAATAACGTGGTGAGGGTAATCGCTGGGCGaac < 2:136782/137‑1 (MQ=255) gcggcgCAGCTTCTTAACAGACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGATGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg < 2:619305/143‑1 (MQ=255) cggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcgc > 1:1089450/1‑143 (MQ=255) cAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaaa < 1:964219/143‑1 (MQ=255) gCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACa > 1:837751/1‑143 (MQ=255) tcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTACCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt < 2:341629/143‑1 (MQ=255) aaCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAACTTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTACTCGCTGGGCGAACAACCGACATGGCGCAAAAACAGGTTGGc > 2:690197/1‑143 (MQ=255) cAAACTGCGCCGCCAGATTTNTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaa > 1:342870/1‑143 (MQ=255) aaaCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaaa > 1:537860/1‑143 (MQ=255) | GTCTCCCGCTTC‑TGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAA > NC_000913/1778237‑1778515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |