| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,856,333 | T→G | 14.6% | E199A (GAG→GCG) ‡ | ydjH ← | putative sugar kinase YdjH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,856,333 | 0 | T | G | 14.6% | 107.5 / 16.3 | 55 | E199A (GAG→GCG) ‡ | ydjH | putative sugar kinase YdjH |
| Reads supporting (aligned to +/- strand): ref base T (26/21); new base G (5/3); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-02 | |||||||||||
CGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCG > NC_000913/1856191‑1856474 | cgccgGCCCCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAAGCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCt < 2:570457/143‑1 (MQ=255) ccTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTATGCTTCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaaa < 1:1096113/143‑1 (MQ=255) tCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTa < 1:803009/143‑1 (MQ=255) cATCGTCATGCCACCCCGCTCGATACAGCAGCCTGCATTACCCGTTTTAACCACCCCCGTTTTTACGCCGTACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg < 1:369761/143‑1 (MQ=255) aTCGTCATGTCACCACGCTCGATCAAGCACCCCACTCAATCTTGATGTGGCGCCACCGTTTTTACGCCGCTCGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact < 1:248658/143‑3 (MQ=255) gTCATGTCACCGCGCTTGATAAAGCAGCCGGCTTTACCCGTTTTCATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGTTTCCTAATTTTGCCGAGGCAAAATTactctc < 2:739094/143‑6 (MQ=255) tCATGTCACCACGCTTGATAAAGCAGCCGTCTCGACCCGTTTTAATGACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc < 2:662274/143‑1 (MQ=255) caccacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGCGTCATTTTGCCTCGGCAACATTAGGAAGCAGATaa > 1:623038/1‑143 (MQ=255) caccacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa > 1:301954/1‑143 (MQ=255) cgcGCTGGATATTGCAGCCGTCGTGACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCGATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg < 2:430892/141‑1 (MQ=255) cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg > 1:890005/1‑143 (MQ=255) acGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa < 2:37662/143‑1 (MQ=255) acGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTCCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAACTAGTAAACAGAtgaaaga > 2:79703/1‑137 (MQ=255) cGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGCGCGCCAGGTTTCCTCGGCAAAATTAGGCAACACACAATCGAc > 2:486875/1‑143 (MQ=255) gCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACa < 1:1030198/143‑1 (MQ=255) gcagcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGGGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg < 1:1052352/143‑1 (MQ=255) gcagcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAACATTAGGAAACAGATAATCGACACACCTCAATg > 1:757552/1‑143 (MQ=255) gcagcCGTCTTTACCCGTTTTAAGCACCACCGTTTGTACGCCGCACGCAAGAAAGCAGTCAGCAATGTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg < 1:182531/143‑1 (MQ=255) tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAATCAATGCTTGGCAAata > 1:718705/1‑143 (MQ=255) ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCCGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatca < 1:417819/143‑1 (MQ=255) cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAAGTTTGCCTCGTCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCTCAAATatcat > 2:302881/1‑143 (MQ=255) gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTa < 2:858796/99‑1 (MQ=255) gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTa > 1:858796/1‑99 (MQ=255) ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCCAATATCATcc > 1:468041/1‑143 (MQ=255) accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAACATTATGAAACAGATAATCGACATAACTCAATGCATCGCAAATATCAACCAGCGTttc > 2:662469/1‑143 (MQ=255) accGGTGTTACGCCGCCCTCAAGAAATCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt < 2:347000/143‑1 (MQ=255) gTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCaa < 1:72193/143‑1 (MQ=255) tACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCCAAATTAGGAAACAGTTCATCGACACAACGCAACGCTTCGCAAATATCATCCAGCGTTTCCTTCAACcgcg > 1:466229/1‑143 (MQ=255) aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg > 2:97621/1‑143 (MQ=255) cacgcaATAAAGCAGTCCGCAATTTCATCCAGTTGCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAACCAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt < 2:413791/143‑1 (MQ=255) caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGTAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCATTTCATTCACCCGCGGgtttctcttt > 1:773483/1‑136 (MQ=255) aaGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCCACCGCGGTTTGATCagat > 1:865369/1‑143 (MQ=255) gAAAGCAGTCAGCAACTTCATCCAGTGTCTCTTTCCCGCTGAGTAATTTTGCCTCCGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCg < 1:835582/143‑1 (MQ=255) aGCAGTCTGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGca < 1:221209/143‑1 (MQ=255) gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcac > 2:448989/1‑143 (MQ=255) tCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACCTAACTCAATGCTTCGCAAATCCCATCCCGCGCTTCATTGAACCGCGGTTTGCTCAAAGCGACACAGac > 2:437997/1‑142 (MQ=255) tCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATCTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGATGCATTCAACCGCGGGTTGATGATATCGGCACAGAt > 2:362152/1‑143 (MQ=255) aattCAGCCAGTGGCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCACTGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAatcat < 2:232674/141‑1 (MQ=255) tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTg < 1:730812/143‑1 (MQ=255) cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGc < 1:476546/143‑1 (MQ=255) gtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCAGTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCttt > 1:615028/1‑143 (MQ=255) tctcTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGAACAGATAATCATCTGCCGGGCTTTa > 1:681139/1‑143 (MQ=255) ctctTTCCCGGTGAGTACTATTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAg < 2:1011310/143‑1 (MQ=255) ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGGCGGGCTGTAg > 1:32638/1‑143 (MQ=255) tctTTCCCGGTGAGTAATTTTGCCTCGGCGAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCACAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCAAAGATAATCATCTGCCGTGGTTTAgg > 2:45515/1‑142 (MQ=255) tttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCCGACGGGCTTTAGCtt > 2:1019282/1‑143 (MQ=255) ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGt > 2:72041/1‑143 (MQ=255) gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCCATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCCTCTGCCGGGCCTACGCTTGCGTAAAACtt > 2:113076/1‑143 (MQ=255) ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTGTCGCTTGAGTAAAGAtt > 1:32395/2‑143 (MQ=255) ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGAGGTTTGAACATATCGGCAGAGACATTCACCGCCCGGACTTAAGCCTGCGCAACAAta > 2:532379/2‑142 (MQ=255) tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCCTTCAACCGCGGTTTGCTCCCATCGTTACAGATAAGCATCTGCCGGGCTTTAGCTTGCGTCAAAAttt > 2:658693/1‑143 (MQ=255) tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATATGCTTGGCTTTAGCTTGCGTAAAACttt > 1:689934/1‑143 (MQ=255) ttttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTg > 1:81687/1‑143 (MQ=255) ttttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGCCCCAGATAATCATCTCCCGGGCTTTATCTTGCGTAAAAATTTcat > 2:861084/1‑141 (MQ=255) ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgc < 1:191681/143‑1 (MQ=255) cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgcg < 1:97621/143‑1 (MQ=255) | CGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCG > NC_000913/1856191‑1856474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |