Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,883,027 C→G 5.5% intergenic (+30/‑161) dmlA → / → yeaV D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,883,0270CG 5.5% 195.3 / 2.7 68intergenic (+30/‑161)dmlA/yeaVD‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base G (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

GCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGT  >  NC_000913/1882890‑1883167
                                                                                                                                         |                                                                                                                                            
gcagcaATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAACATTATTTTGCGTTAAGGTCAAACCCGTTTATTTGCACCGCGTCACTGTcg                                                                                                                                         >  2:365576/1‑143 (MQ=255)
  agcaATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgcg                                                                                                                                       >  1:468488/1‑143 (MQ=255)
  agcaATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCCCGTCACTGAcgcg                                                                                                                                       >  2:822250/1‑143 (MQ=255)
       ttGAAGAAGTGATTGCTCACGGGCCGACAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCGGttca                                                                                                                                  <  2:601821/143‑3 (MQ=255)
        tGAAGAAGTGATTGCTCACGTGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTACGGTCCAACCAGTTTATTTGAACCGCCTCACTGACGCGGgtttt                                                                                                                                 >  2:911560/1‑143 (MQ=255)
        tGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGttttt                                                                                                                                 <  1:702129/143‑1 (MQ=255)
        cgaaggaGGGATTGCTCACGGGCCGAAAACACCTGAGATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGttttt                                                                                                                                 <  2:43563/142‑1 (MQ=255)
           agaggTGAGTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTa                                                                                                                              <  1:892360/143‑1 (MQ=255)
           agaagTGATTGCTCACGGGCCGAACACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTCTTTTGCGTTAAGGTCAAACCCGTTTATTTGAACCGCGTCACTGCCGCGGATTTTTTa                                                                                                                              >  2:194964/1‑143 (MQ=255)
                    tGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCtt                                                                                                                     <  1:848905/143‑1 (MQ=255)
                      cTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTg                                                                                                                   <  2:517818/143‑1 (MQ=255)
                       tCACGGGCCGAGAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGc                                                                                                                  <  2:203135/143‑1 (MQ=255)
                       tCACGGGCCGAAAACACGTGATATGAAAGGCAATGCCACCGCGCCACAGGTTGCCGACGCGATTTGCAAAATAATTTACCGTTAAGGTACGAGCGGGTTCTTTGAACCGCGTCACTGAAGCGGTTTTTTTATTGGTTGTTTGc                                                                                                                  >  2:663297/1‑143 (MQ=255)
                        cACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCa                                                                                                                 <  1:1021032/143‑1 (MQ=255)
                         aCGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAg                                                                                                                >  1:786905/1‑143 (MQ=255)
                           gggCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTa                                                                                                              >  1:1099396/1‑143 (MQ=255)
                            ggCCGTCAACACCTGATAGCTATGGCAACGCCACCGCGCCACAGGTTGCCGAGGCGATTTGCAAAATTATTTTGCGTTAAGGTCACACCAGTTTATTTCAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTaa                                                                                                             <  1:838497/143‑1 (MQ=255)
                             gCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGAGTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTCATTTGAACCGCGTCAATGACGCGGTGTTTTTATTCAGTCTTTGAAGGaac                                                                                                            >  2:336113/1‑142 (MQ=255)
                              ccGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTaagt                                                                                                           >  2:287688/1‑141 (MQ=255)
                              ccGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGGCACTGACGCGGTTTTTTTATTCGTTGTTTGCAGTAAAt                                                                                                           >  2:900375/1‑143 (MQ=255)
                                gAAAACACCTGATATGAAAGGCAATGCCACCTCGCCACATGTTGCCTTTGCGTTTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATaa                                                                                                         <  1:79968/143‑1 (MQ=255)
                                     cacCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGc                                                                                                    <  1:667207/143‑1 (MQ=255)
                                      acCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCCAGCAAGTACGTCGAAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCg                                                                                                   >  1:253930/1‑143 (MQ=255)
                                      acCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACGTGCg                                                                                                   >  1:975189/1‑143 (MQ=255)
                                      acCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCg                                                                                                   >  1:1086107/1‑143 (MQ=255)
                                         tGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATATGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGAAGTAAATAACCGACGTCa                                                                                                >  2:62785/1‑143 (MQ=255)
                                              tGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGATCGTGTTGTTGGTTCTTTGCAGTAAATAACCTGCGTCAGTTCa                                                                                           >  1:562758/1‑143 (MQ=255)
                                                   ggCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCttt                                                                                      <  1:545788/143‑1 (MQ=255)
                                                    gCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCGAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCtttt                                                                                     >  1:315651/1‑143 (MQ=255)
                                                     cAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCttttt                                                                                    >  2:1084810/1‑142 (MQ=255)
                                                       aTGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTAtt                                                                                  >  1:300038/1‑143 (MQ=255)
                                                           caccacGCCACAGGTTGCCGACGCGATTTGCAAAATTATCTTGCGTTCAGGTCAAACCAGTTTATTTGAACCTCGTCACTGACGCGGTATTTTTACTCGTTCTTTGCAGTCACTAACCTGCGTCATTTCACCTTTCATTGttc                                                                              >  2:13943/1‑142 (MQ=255)
                                                                  ccACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTc                                                                       >  2:311103/1‑143 (MQ=255)
                                                                   cacaGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCg                                                                      >  1:15704/1‑143 (MQ=255)
                                                                        gTTTCCGCCGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGATTTTTTTATTAGTTCTTTGCAGTAAATAACCTGCGTCATTTCACATTTTATTGTTTACGTTTCGAGttt                                                                 >  2:344155/1‑143 (MQ=255)
                                                                           gCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGACCCGCGTCACTGACGCGGTTTTTTTATTCGGTCAATGCCGTAAATAACCTGCGCCATTTCACCTTTTATTGTTTCCGTTTCGTCTTTTAt                                                              >  1:1098823/1‑143 (MQ=255)
                                                                           gCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTAt                                                              >  1:1094336/1‑143 (MQ=255)
                                                                            ccGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                                             >  1:609539/1‑143 (MQ=255)
                                                                            ccGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                                             >  1:565355/1‑143 (MQ=255)
                                                                                   gATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGCTTTATGGCTTTcg                                                      >  2:150248/1‑142 (MQ=255)
                                                                                      ttGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTa                                                   >  2:193637/1‑143 (MQ=255)
                                                                                        gCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTAtt                                                 <  1:822250/143‑1 (MQ=255)
                                                                                        gCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCCTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTAtt                                                 <  1:188158/143‑1 (MQ=255)
                                                                                          aaaaTTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCt                                               >  1:606889/1‑143 (MQ=255)
                                                                                           aaaTTATTTTGCGTTAAGGTCAAGCCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCtt                                              <  1:1073203/143‑1 (MQ=255)
                                                                                             atcattTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTaa                                            <  2:294340/140‑1 (MQ=255)
                                                                                               tcttttGCGTTAAGGTCAAATCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAtt                                          <  2:104066/141‑1 (MQ=255)
                                                                                               tattTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAtt                                          <  1:729445/143‑1 (MQ=255)
                                                                                                  tttGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGtt                                       <  1:329096/143‑1 (MQ=255)
                                                                                                    tGCGTTAAGGTCAAACCAGTTTATATGAACCGCGTCACTCACGCGTTTTTTTTATTCGTTCTTTGCAGTAAAGAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTa                                     <  1:802080/143‑1 (MQ=255)
                                                                                                      cGTTAAGGTCAAACCAGTTTATTTGATCCGTGTCATTGTCGTGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAAt                                   <  1:143368/143‑1 (MQ=255)
                                                                                                      cGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAAt                                   <  1:693784/143‑1 (MQ=255)
                                                                                                          aaGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt                               <  1:592360/143‑1 (MQ=255)
                                                                                                          aaGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt                               <  2:362381/143‑1 (MQ=255)
                                                                                                             gTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTa                            >  2:795023/1‑143 (MQ=255)
                                                                                                              tCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTaa                           >  2:166744/1‑143 (MQ=255)
                                                                                                                         tATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAAttt                <  2:606889/143‑1 (MQ=255)
                                                                                                                          aTTTGAACCGCGTCACTCTCGTGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAAtttt               <  1:685792/143‑1 (MQ=255)
                                                                                                                           tttGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTCACATGCAAATTTTg              >  1:87075/1‑143 (MQ=255)
                                                                                                                           tttGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTg              <  1:193637/143‑1 (MQ=255)
                                                                                                                            ttGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGt             <  1:311103/143‑1 (MQ=255)
                                                                                                                             tGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTAGTGTTTCCGTTTCGTGTTTTATGGCTTTCCGGATTCTTAAGTGTTTACTTTATGTAACATGCAAATTgtgtg            >  1:390664/1‑142 (MQ=255)
                                                                                                                              aaaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAATTTTTGTTa           >  2:413947/2‑143 (MQ=255)
                                                                                                                               aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTTTGGCACCCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAc          >  1:1096315/1‑143 (MQ=255)
                                                                                                                                aCCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAcg         <  1:385178/143‑1 (MQ=255)
                                                                                                                                 ccGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATATCCTGCGTCATTTCTCCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAcgc        <  2:721520/143‑1 (MQ=255)
                                                                                                                                  cgcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAcgcg       >  2:329492/1‑143 (MQ=255)
                                                                                                                                       taCTGGCGCGGTTTTTTTATTCGTTCTTTGCAGTAAATATCCTGCGTCTTTTCACCTTTTATTGTTTCCGGTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGt  <  2:280220/142‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCGTACGT  >  NC_000913/1882890‑1883167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑