Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 114,507 A→T 9.9% intergenic (+20/+15) guaC → / ← hofC GMP reductase/inner membrane protein HofC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5070AT 9.9% 122.7 / 5.6 52intergenic (+20/+15)guaC/hofCGMP reductase/inner membrane protein HofC
Reads supporting (aligned to +/- strand):  ref base A (26/18);  new base T (2/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

ATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCG  >  NC_000913/114365‑114649
                                                                                                                                              |                                                                                                                                              
atTTTGGGCTGCTCGCGCTCAGCTGTTACATACGTTGGGGCTTCACGCCTGAAAGAGCTAACCAAGCGCTCCACGTTTATTCGTGTGCAGGAACAAGCAAACCGCATCTTCAACAACCTGTTATCTCCCATCGCTGGCGTGGa                                                                                                                                                <  2:164338/143‑1 (MQ=255)
atTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGa                                                                                                                                                <  1:546150/143‑1 (MQ=255)
     gggTGGCGTGCGTTGAGCCTGTACTTACGTTGGCGCTTAACGCGGGTATGTGCTGACCAAGCGCACCACTTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAac                                                                                                                                           <  2:781309/143‑1 (MQ=255)
           ccTGCGTTCAGCTTGTACCTACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCa                                                                                                                                     <  1:704625/143‑1 (MQ=255)
           ccTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCGGTAATCTCCCAACGCTGGCGTGGAGCAACACGcaa                                                                                                                                     >  2:999031/1‑141 (MQ=255)
             tGCGTTCAGCTTGTACATGCGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGc                                                                                                                                   <  2:766976/143‑1 (MQ=255)
             tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACACGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGGGGCGCCACACGCCAGc                                                                                                                                   >  2:613502/1‑143 (MQ=255)
             tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGc                                                                                                                                   >  2:565659/1‑143 (MQ=255)
               cGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGt                                                                                                                                 <  2:123680/143‑1 (MQ=255)
                gTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCAGACCCGATACATTTTTGTTCTTCGTGGGCAGTAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGtt                                                                                                                                <  2:784404/143‑1 (MQ=255)
                 ttCAGCTTGTACATTCGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGttg                                                                                                                               <  1:860854/143‑2 (MQ=255)
                   cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatc                                                                                                                             >  1:126251/1‑143 (MQ=255)
                       ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACCACCTGTAATCTCCCAAAGATGGGGGCCTGCATCACGCCAGCGTTatcccat                                                                                                                         >  1:984415/1‑143 (MQ=255)
                                 gTTGGGGCTTCACGCCTGAAAGAGCTGCCCAAGCGGACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatc                                                                                                               <  2:10528/143‑1 (MQ=255)
                                      ggCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAACCCGCAGCTTCAACAACCTGTAATCTCCCAACGCCGGCGTGGAGCACCACGCCAGCGTTATCCCATCACCCCcaccgcatc                                                                                                          >  2:1099276/1‑143 (MQ=255)
                                            aCGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTCTCCCATCCCACTCATCGCATCGCCTaa                                                                                                    >  1:92644/1‑143 (MQ=255)
                                            aCGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAAACTGTAATCTCCCAACGCAGGCGTGCATCAACACGCCAGCGTTATCCCATCCCACTCATcgcctcgcctca                                                                                                    >  1:1046348/1‑141 (MQ=255)
                                                cTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTCAATgg                                                                                                >  1:712036/1‑143 (MQ=255)
                                                  gAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTACATGGaa                                                                                              >  2:706543/1‑143 (MQ=255)
                                                     agagCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGCAAAt                                                                                           >  1:838019/1‑143 (MQ=255)
                                                       agCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTg                                                                                         >  1:3787/1‑143 (MQ=255)
                                                       agCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTg                                                                                         <  2:126251/143‑1 (MQ=255)
                                                            aCCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCCACATGGAAAAATGGCAgc                                                                                    >  1:177523/1‑142 (MQ=255)
                                                              cAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATa                                                                                  >  1:769724/1‑143 (MQ=255)
                                                                 gcgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAt                                                                               <  2:598870/143‑1 (MQ=255)
                                                                  cgcACCGCGTGTCTTCGTGGGCAGGAACCAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:170106/143‑1 (MQ=255)
                                                                  cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:907642/143‑1 (MQ=255)
                                                                  cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  1:829508/143‑1 (MQ=255)
                                                                  cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:560303/143‑1 (MQ=255)
                                                                     tgcacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTGCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACGCATCGCATCGCCTAAATGGAAAATTGGCAGATACACTGcc                                                                           <  2:25794/141‑1 (MQ=255)
                                                                     accacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGACGTGGAGCAACACGCCAGCGTCATCCCATCCACCAGAAACCAGCGCCTAAACGGAAAATTGGCCGATCCATTGcc                                                                           >  2:779173/1‑143 (MQ=255)
                                                                          gTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccac                                                                      >  1:980725/1‑143 (MQ=255)
                                                                              aTTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGc                                                                  >  2:135545/1‑143 (MQ=255)
                                                                                  gtgtGCAGGAACAAGACAACCGCAGCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTAc                                                              <  1:32254/143‑1 (MQ=255)
                                                                                    gtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCAGAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCACCTAAATGGAAAATTGGCAGGTACATTGCCACCACCAGCGTACCa                                                            >  1:306819/1‑143 (MQ=255)
                                                                                      gCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaat                                                          >  2:954386/1‑143 (MQ=255)
                                                                                       cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAACGGAAAATTGGCAGATACATTGCCACCCCCCGCGTAGCaatc                                                         >  2:1028817/1‑142 (MQ=255)
                                                                                        aGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaataa                                                        <  1:847776/143‑1 (MQ=255)
                                                                                               aGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATtcctcc                                                 >  2:436124/1‑143 (MQ=255)
                                                                                                gAAAACCGCATCTTCAACAACCTGTAATCTCCCAGCGCTGGCGTGGAGCAACACGCCAGCGTTACCCCATCACACTCATCGCATCGCGTAAAGGGCAAATTGGCAGATGCATTGCCACCACCAGCGTACCACTAGTTCCTccc                                                >  1:132882/1‑143 (MQ=255)
                                                                                                       gCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAACTGGAAAATTGGCAGATACATTGCCACCACCAGCGTCCCCCTACTCCCCCCCGTTCTGa                                         >  1:762686/1‑143 (MQ=255)
                                                                                                        cATCTCCCACGACCTGGAATCTCCCCCCGCTGGCGTGGCGGAACGCGCCAGCGGTATCCCATCCCACTCGTCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGAt                                        <  2:1059237/143‑1 (MQ=255)
                                                                                                         aTCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATc                                       <  1:1000510/143‑1 (MQ=255)
                                                                                                          tCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                      <  1:565659/143‑1 (MQ=255)
                                                                                                              caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATAATCAGCaa                                  >  1:708219/1‑143 (MQ=255)
                                                                                                                    cctgccaTCGCCCACCGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAAGTCCTCCCGTTATGATCAGCAACGCCgg                            <  2:736532/137‑1 (MQ=255)
                                                                                                                        taaccccccAGCGCCGGCGGGGAGCAACACGCCAGCGTTATCCCACCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATTCCTCCCGTTATGATCAGCAACGCCGGTTcc                        <  1:817795/137‑1 (MQ=255)
                                                                                                                              cccAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAg                  >  2:928376/1‑143 (MQ=255)
                                                                                                                                cAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGc                >  1:421744/1‑143 (MQ=255)
                                                                                                                                     cTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCCGATACATTGCCACCCCCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGCCTGcaa           >  1:523063/1‑141 (MQ=255)
                                                                                                                                     cTGGCGTGGCGCAACACGCCAGCGGTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGa           <  1:999031/143‑1 (MQ=255)
                                                                                                                                      tGGCCTGGACCCACCCGCCCGCGTTATCCCTTCCCACTCCTCGCATCGCCTAAATGGAAAATTGTCATATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAg          <  2:894302/143‑1 (MQ=255)
                                                                                                                                           tGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTAt     >  2:351277/1‑143 (MQ=255)
                                                                                                                                             gAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGGTCCAGTCAGGCTGCGAGGTCATcc   >  2:368998/1‑143 (MQ=255)
                                                                                                                                              aGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCGAAATGGAAAATTGGCAGATACATTGCCACCACCACCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTACGGCTTCGATGttcaccg  >  2:187422/1‑138 (MQ=255)
                                                                                                                                              |                                                                                                                                              
ATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCG  >  NC_000913/114365‑114649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑