| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,004,228 | G→T | 8.1% | T119T (ACG→ACT) | fliD → | flagellar filament capping protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,004,228 | 0 | G | T | 8.1% | 137.2 / 2.9 | 58 | T119T (ACG→ACT) | fliD | flagellar filament capping protein |
| Reads supporting (aligned to +/- strand): ref base G (29/23); new base T (2/3); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAA > NC_000913/2004087‑2004370 | caccagcaccaccagcaGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACAATCAGCGACACCCATCTGGCGCAGGCGCAAACCCTGACGACGCGCACGAAGAGAGAAGATAGGAAAAggg > 1:255008/1‑143 (MQ=255) agcaccaccagcaGCACCACCGCGTTCAGTGCCACCACTCCGGGAAACGCCATCGCCGGGAAATACACCATCAGCCTCACCCGTCTCGCGCAGGCGCAAACCCAGACCACGCGCCCCACCAGAGACAAGACGAAAACAGCgca > 2:705030/1‑141 (MQ=255) caccagcaGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGCGACGCTACGAAACCGGCGCTCGcca > 1:923174/1‑143 (MQ=255) ccagcaGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCGCCACCCGAGACGCTACGCAAACGGCGAGCgaaagc > 2:161029/1‑138 (MQ=255) ccagcaGCACCACAGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGACGCAAACCCAGACCACGCGCACCACCAGAGACGCAACGAAAACGCAGACCGccaca > 2:286324/1‑142 (MQ=255) cagcagCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGccacca > 1:2554/1‑143 (MQ=255) cgcagcaCCACCGCGTGCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAg < 2:740637/142‑1 (MQ=255) agcagcACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAACACGGCGATCGCCACGAg > 1:268203/1‑143 (MQ=255) caccacCGCGTTCAGTGCCACCCCTGCGGGTAACGCCACCGCCGGGAAATACCCCATCAGCGTCACCCAGCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACa < 2:265292/143‑1 (MQ=255) ccGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGTGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTaaa < 2:557137/143‑1 (MQ=255) ccaccaCTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTcaa > 1:468617/1‑143 (MQ=255) ccaccaCTGCGGGTAACGCCACCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGCCAGCCACCAGCGATAGTAAACTCACCATTcaa < 2:673880/143‑1 (MQ=255) ggTAACGCCATCGCCGGGACACACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGCCCACGCGCACCACCAGAGACGATACGAAAACTGTGTTCGCCACCAGAGACAGTAAACTCACCATTCAACAAGGCGGCGa < 1:160415/143‑1 (MQ=255) ggTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATGTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCGGCGACAGTGAACTCACCGTTCAACAAGGCGGCGa > 1:419754/1‑143 (MQ=255) ggTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCACACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGa < 1:1085395/143‑1 (MQ=255) accGCCATCGCCGCGAAATTGCCCATCAGCGTCACCCATCTGGCGCAGGCGCAAGCCCTGACCACTCGTCCCACCACCGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACaa < 2:835557/141‑1 (MQ=255) cGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAg < 2:798633/143‑1 (MQ=255) cGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAg < 1:887763/143‑1 (MQ=255) gCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAAAGACGATATGTAAACGGCGCTCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACACAGa > 1:302632/1‑143 (MQ=255) gCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTCGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATCCGAAACCGGCGATCGCCACCAGCGACAGTAAACTAACCAGTCAAGAGGGCGGCGACAAAGa > 1:293549/1‑143 (MQ=255) ccGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACTGCGATCGCCACCAGCGACAGTAAACTCACCAtt > 1:58234/1‑116 (MQ=255) ccGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACTGCGATCGCCACCAGCGACAGTAAACTCACCAtt < 2:69656/116‑1 (MQ=255) ccGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACTGCGATCGCCACCAGCGACAGTAAACTCACCAtt > 1:69656/1‑116 (MQ=255) ccGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACTGCGATCGCCACCAGCGACAGTAAACTCACCAtt < 2:58234/116‑1 (MQ=255) cGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCAGTCAACAAGGCGGCGACAAAGATACgcgta > 1:813186/1‑139 (MQ=255) gAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGCCGATACGAAAACGGCGATCGCCACCAGCGACAGAAAACTCACCATACACCAAGACAACGCAAAAGATCCGagcaccc > 2:529198/1‑137 (MQ=255) aaTACACCATCAGCGCCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGATAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCAtt < 1:659938/143‑1 (MQ=255) tCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCACTCAACAAGGCGGCGACAAAGATCCGATGACCATTGATATCAgc > 2:135871/1‑143 (MQ=255) gggcgctcATCTCGCGCAGGCGCAGAGCCTGTGCGGGGGCAGCACTGGAGACGATAAGAAAGCGGCGGTGGCCACCGGCGACAGTAAACTCACCATTCAACAAGGCGGCGACGAAGATCCGATTACCATGGATATCAGCGCgg < 2:422224/136‑1 (MQ=255) aCCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTaa < 1:1024161/143‑1 (MQ=255) ccATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCCCCAGCGGCGATGCGAAAGCGGCGATCGCCACCAGCCACAGTACACTCACCCATCAACAAGGCGGAGACAAACAAGCGGTCGCCATTGAGAACAGCGCGGCGaaag > 2:331560/1‑141 (MQ=255) ccATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCCCCAGAGACGATACGAAAACGGCGATCGCCACCAGCGCCAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGGTATCAGCGCGGCCaatt > 1:743277/1‑141 (MQ=255) ccATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATCCGCAAACGGCGATCGGCACCAGCGAGAGTGGACGAGCCACTCAACAAGGAGGCGACACAGAAGCGATGACAACTGATATCAGCGCGGcgacgt > 2:331564/1‑138 (MQ=255) tCTGGCGCAGGCGCAAACCCTGCCCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCAt < 2:1017761/143‑1 (MQ=255) tCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGCTATCAGCGCGCCTAACGCAt > 1:644594/1‑143 (MQ=255) cTGGCGCAGGCGCAAACCCCGACCACGCGCCCCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCTACAAAGATCCGATTACCATTGAGATCAGCGCGGCTAACTCATc < 1:103902/143‑1 (MQ=255) tGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCg < 1:456091/143‑1 (MQ=255) ggcgcaggcgcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCAATGATATCAGCGCGGCTAACTCATCGt > 1:898923/1‑143 (MQ=255) gcaggcgcaAACCCTGACCACGCGCACCCCCCGAGACGACACCAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTaa < 1:461955/143‑1 (MQ=255) aggcgcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGCAAACTCACCAACCAACAAGCCGCCGACAAACATCCGACTACCACCGACAACAGCGCACCTACCTCATCGATCAGc > 1:496414/1‑143 (MQ=255) ggcgcaAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCg < 1:87050/143‑1 (MQ=255) cccaAACCGTGATCACTCGCACCGCCAGAGACGCTACGAAAACGGCCATCGCCACCATCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAATATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCggg < 2:118982/141‑1 (MQ=255) cTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGa < 2:2554/143‑1 (MQ=255) tGACGACGCGCACCACCAGAGACGATACGACAACGGCGATCGCCACCAGCGCCAGCAAACTCACCATCCAACAAGGCGGCGACGCAGATCCGCGTACCATTGATATCAGCGCGCCACACCCATCGTCAAGCCAGACCCCTGAt > 2:1030455/1‑143 (MQ=255) aCCCCGCTCACCGCCAGAGACGATACGAAAACGGCGATCGCCGCCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGc < 2:268852/143‑1 (MQ=255) ccACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGcc > 1:347049/1‑143 (MQ=255) ccACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATCACCATTGATATCAGCGCGGATACCTCAACGTTAAGCGGGATCCGTGATGcc > 1:201202/1‑143 (MQ=255) caccccCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATcaaca < 2:268203/143‑1 (MQ=255) caccaGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACg > 1:599791/1‑143 (MQ=255) gagaCGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCaaaa < 1:1015302/143‑1 (MQ=255) aCGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATGACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGAGCCGTGATGCCATCAACAACGCAAAAGCa > 2:1057606/1‑143 (MQ=255) ggacaCGGAAACGGGGACCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAg < 1:705030/139‑1 (MQ=255) gATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAgg > 1:887790/1‑143 (MQ=255) tACGAAAACGGCGATCGCCACCAGCGACAGAGAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATGTCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGAAGGCg > 2:401320/1‑143 (MQ=255) caCGAAACCGGCGACCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCg < 1:1057809/142‑1 (MQ=255) gAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGGGATGCCCTCACCAACGCAAAAGCAGGTGTaa > 1:138632/1‑143 (MQ=255) gAAAACGGCGATCGCCACCAGAAACAGTAAACCCACCACTCAACAAGGCAACGACGAACATCCGAGCACCAATGATAACACCGCACCTCACACAACGGTAAACGGCAACCGTGAACGCAAGAACAACGCAAAAGgcggcggcg > 2:274353/1‑140 (MQ=255) aaaaCGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACGAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAg > 1:578995/1‑143 (MQ=255) aaaCGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGAAAAAGCAGGCGAAAga > 2:352750/1‑142 (MQ=255) ggCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCaa > 1:527167/1‑143 (MQ=255) | CACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCATCGCCGGGAAATACACCATCAGCGTCACCCATCTGGCGCAGGCGCAAACCCTGACCACGCGCACCACCAGAGACGATACGAAAACGGCGATCGCCACCAGCGACAGTAAACTCACCATTCAACAAGGCGGCGACAAAGATCCGATTACCATTGATATCAGCGCGGCTAACTCATCGTTAAGCGGGATCCGTGATGCCATCAACAACGCAAAAGCAGGCGTAAGCGCAA > NC_000913/2004087‑2004370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |