Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 12.6% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT12.6% 106.1 / 11.9 51I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base T (4/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03

AAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAA  >  NC_000913/2081549‑2081826
                                                                                                                                           |                                                                                                                                          
aaGGGTATATTTGGCTTTGATATGTGGAATTGCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAttt                                                                                                                                         <  2:67113/143‑1 (MQ=255)
   ggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCCTACCCGATGGGTACCCCTCCGCCCTTTTTCCCACGGTGCAAACTTTGcc                                                                                                                                      >  2:778607/1‑142 (MQ=255)
    gTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                                                                     <  1:972750/143‑1 (MQ=255)
        ctttGGCTTTGGTCTGTGGGATTTCGGCTTTCGGGAGCACCATCCAGGACTCGTGTTCCGTTCCCGCGGCGATCTTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgta                                                                                                                                 <  2:1019256/142‑1 (MQ=255)
        atTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                 <  1:656543/143‑2 (MQ=255)
        atTTGGCTTTGATATGTGGAATTTCGGCTTGCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                 <  2:211028/143‑2 (MQ=255)
               tttGGTATGTGGAATTTCGGCTTTCGGTAGCCCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGt                                                                                                                          >  1:761105/1‑143 (MQ=255)
               tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCACAATTTCCACCctacttgagt                                                                                                                          >  1:685481/1‑136 (MQ=255)
                ttGATATGTGGAATTTCTGCTTTTGGTAGCACTATTCGGTATACGTGTTGTTTTCCCCGGTCGATATTTTCTTTATCGCCATATCATATGCGTTCCGTTCCGACCTTTTTCCCATGGTGCAAAATTTGCACCGTAGTAAAGtt                                                                                                                         <  2:230253/143‑1 (MQ=255)
                   atatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                                                                      <  1:287043/143‑1 (MQ=255)
                    tatGTGGCAGTTCGGCTGTCGGTAGCACCATCCAGACCTCTTGTTCCGTTCCCCGGTCGATCTTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                     <  2:1105502/143‑1 (MQ=255)
                    tatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                     >  1:738484/1‑143 (MQ=255)
                    tatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTCTTTCACACAGTGCCACATTCGCCCCGCAGTAAACGTttgt                                                                                                                     >  1:1068675/1‑143 (MQ=255)
                       gtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATACATTCTGTTCCGACCTATATCCCACGGTGCAAAATTTGCACCTTAGTAAAGTTTTGTTGc                                                                                                                  >  1:566469/1‑143 (MQ=255)
                            aTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCa                                                                                                             <  1:334633/143‑1 (MQ=255)
                             tctCGTCTTTCAGTAGCACCATCCAGAACTCTTGTTCCGTTCCCATGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCaa                                                                                                            <  2:1014842/141‑1 (MQ=255)
                              ttCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAAc                                                                                                           >  2:449131/1‑143 (MQ=255)
                                  gCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCCCCATACCAGATGCGTTCCGTTCGGACCTTTTTCCTAAGATGCAAAATTTACACAGTAGTAAAGTTTTGTTGCCACCAACGCAg                                                                                                       >  1:98824/1‑143 (MQ=255)
                                   cTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCCCGCTGCAAAATTTGCACCGTAGTAACGTTTTGTTGCCCCCAACGccct                                                                                                      >  1:485963/1‑140 (MQ=255)
                                        ggTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTGCCTCGGTGCGGAATTTGCCCCGTGGTGATGTTTTGTTGCACCCGTCGGAGTAAttt                                                                                                 >  1:65402/1‑143 (MQ=255)
                                            tgaCCATCCAGAGCGCCTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGCCCTCTTTCCCACGGTGCAAAATTGGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                             <  2:835425/141‑1 (MQ=255)
                                              aCCATCCAGAACTCTTGTTCCGTTCCCAGGTTGGTATTTTCGTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                                                           <  2:1042938/143‑1 (MQ=255)
                                                   ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                                                                                      >  1:596607/1‑143 (MQ=255)
                                                    taGATCTCTTTTTCCGTTCCCAGGTCGTTATTTTCTTTATCGCCATACGAGATGCGTTCCGTTCTGCCGGGTGGCCCAGCGTGGACAAGTTTTTCCGTAGTAAAGTTTTGTTGCCCCGGACGCAGTAATTTTCTTGCCTCTTc                                                                                     <  2:234923/142‑1 (MQ=255)
                                                        tctctCGTTCCGTTCCCAGGTCGATATGTTCTTGGTCGCCATCCGAGGTGCGTTCCGTTCCGAGCTTTTTCCCACGGTGCAGAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                                                                 <  1:886595/142‑1 (MQ=255)
                                                                    ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                     <  2:596607/143‑1 (MQ=255)
                                                                              gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACAGTAGTCAAGTTTTGTTGCCCCCAACGCAGTAATTATCTCGCCTCTTCCTCACGACCTTTGACACTCTCAGccc                                                           >  2:1055626/1‑143 (MQ=255)
                                                                               atatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc                                                          >  1:140144/1‑143 (MQ=255)
                                                                                  ttttCTTTACTGCCCTTCCAGCTGCGTTCCGTTCCGATCTTTTTCCCACGGTGCAAACTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt                                                       <  2:677128/143‑1 (MQ=255)
                                                                                  tctgcTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt                                                       <  1:768097/139‑1 (MQ=255)
                                                                                         tctCGCCACACCAGATGCGTGCTGTTCCGACCTCTTTCCCACGGTGCAAAGTGTGCACCGTAGTGAGGTTTTGGTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCGTTTGCACTGTCAGCCCCCATTACCACt                                                <  1:929684/141‑1 (MQ=255)
                                                                                             gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCaa                                            <  2:231811/143‑1 (MQ=255)
                                                                                              ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAAt                                           <  1:7797/143‑1 (MQ=255)
                                                                                                     aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTTCGCCCCCATTCGCACTGCAAGGAgacga                                    >  2:483362/1‑143 (MQ=255)
                                                                                                     aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacga                                    <  1:746523/143‑1 (MQ=255)
                                                                                                       aTGCGTTGCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGacgacg                                  <  2:315427/143‑1 (MQ=255)
                                                                                                         gCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCt                                <  1:788690/143‑1 (MQ=255)
                                                                                                          cgttccgtacCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg                               <  1:405537/143‑1 (MQ=255)
                                                                                                           gttccgtgccGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGc                              <  1:679459/143‑1 (MQ=255)
                                                                                                            ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTCGCCTCTTCCTCACGACCTTTTGCACTGTCCGCCCCCCTTACCTCTGCAATGAGCCGAAGcgggc                             >  2:729438/1‑139 (MQ=255)
                                                                                                                     gACCTTTTTCCCACGGTGCAAAATTAGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTCCCACCGCAATGAAACGACGCATCCCACCTACAg                    >  2:841625/1‑143 (MQ=255)
                                                                                                                         tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCgc                >  2:868018/1‑142 (MQ=255)
                                                                                                                         tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGa                >  1:643137/1‑143 (MQ=255)
                                                                                                                         tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTGTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCCGCCCCCATTACCACTGCAATGAGACGCCGCGGCCCATCTACAGCCgc                >  2:281565/1‑142 (MQ=255)
                                                                                                                                taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTACTTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTCCAGCCGAAGCAATg         >  1:609908/2‑143 (MQ=255)
                                                                                                                                taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTGCCCATCTCCCGCCGAAGCAATg         >  1:485168/2‑143 (MQ=255)
                                                                                                                                taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATg         >  1:968250/2‑143 (MQ=255)
                                                                                                                                taCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATg         >  1:1030787/2‑143 (MQ=255)
                                                                                                                                 aCGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga        >  1:276625/1‑143 (MQ=255)
                                                                                                                                   ggTGCAAAATTTGCACCGCAGTAACGTTTTGTTGCCCCCCACGCAGTAATTTTCTTGCCTCTTCCTCACGACCCTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgaga      <  2:267188/143‑1 (MQ=255)
                                                                                                                                       cAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTGCCCCTCTCCAGCCGACGCAATGAGATTaa  >  2:574818/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAA  >  NC_000913/2081549‑2081826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑