Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 114,511 A→C 8.4% intergenic (+24/+11) guaC → / ← hofC GMP reductase/inner membrane protein HofC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5110AC 8.4% 125.0 / 3.4 52intergenic (+24/+11)guaC/hofCGMP reductase/inner membrane protein HofC
Reads supporting (aligned to +/- strand):  ref base A (27/19);  new base C (2/3);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

GGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCG  >  NC_000913/114370‑114649
                                                                                                                                             |                                                                                                                                          
gggTGGCGTGCGTTGAGCCTGTACTTACGTTGGCGCTTAACGCGGGTATGTGCTGACCAAGCGCACCACTTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAac                                                                                                                                           <  2:781309/143‑1 (MQ=255)
      ccTGCGTTCAGCTTGTACCTACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCa                                                                                                                                     <  1:704625/143‑1 (MQ=255)
      ccTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCGGTAATCTCCCAACGCTGGCGTGGAGCAACACGcaa                                                                                                                                     >  2:999031/1‑141 (MQ=255)
        tGCGTTCAGCTTGTACATGCGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGc                                                                                                                                   <  2:766976/143‑1 (MQ=255)
        tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACACGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGGGGCGCCACACGCCAGc                                                                                                                                   >  2:613502/1‑143 (MQ=255)
        tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGc                                                                                                                                   >  2:565659/1‑143 (MQ=255)
          cGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGt                                                                                                                                 <  2:123680/143‑1 (MQ=255)
           gTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCAGACCCGATACATTTTTGTTCTTCGTGGGCAGTAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGtt                                                                                                                                <  2:784404/143‑1 (MQ=255)
            ttCAGCTTGTACATTCGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGttg                                                                                                                               <  1:860854/143‑2 (MQ=255)
              cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatc                                                                                                                             >  1:126251/1‑143 (MQ=255)
                  ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACCACCTGTAATCTCCCAAAGATGGGGGCCTGCATCACGCCAGCGTTatcccat                                                                                                                         >  1:984415/1‑143 (MQ=255)
                            gTTGGGGCTTCACGCCTGAAAGAGCTGCCCAAGCGGACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatc                                                                                                               <  2:10528/143‑1 (MQ=255)
                                 ggCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAACCCGCAGCTTCAACAACCTGTAATCTCCCAACGCCGGCGTGGAGCACCACGCCAGCGTTATCCCATCACCCCcaccgcatc                                                                                                          >  2:1099276/1‑143 (MQ=255)
                                       aCGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTCTCCCATCCCACTCATCGCATCGCCTaa                                                                                                    >  1:92644/1‑143 (MQ=255)
                                       aCGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAAACTGTAATCTCCCAACGCAGGCGTGCATCAACACGCCAGCGTTATCCCATCCCACTCATcgcctcgcctca                                                                                                    >  1:1046348/1‑141 (MQ=255)
                                           cTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTCAATgg                                                                                                >  1:712036/1‑143 (MQ=255)
                                             gAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTACATGGaa                                                                                              >  2:706543/1‑143 (MQ=255)
                                                agagCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGCAAAt                                                                                           >  1:838019/1‑143 (MQ=255)
                                                  agCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTg                                                                                         >  1:3787/1‑143 (MQ=255)
                                                  agCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTg                                                                                         <  2:126251/143‑1 (MQ=255)
                                                       aCCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCCACATGGAAAAATGGCAgc                                                                                    >  1:177523/1‑142 (MQ=255)
                                                         cAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATa                                                                                  >  1:769724/1‑143 (MQ=255)
                                                            gcgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAt                                                                               <  2:598870/143‑1 (MQ=255)
                                                             cgcACCGCGTGTCTTCGTGGGCAGGAACCAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:170106/143‑1 (MQ=255)
                                                             cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  1:829508/143‑1 (MQ=255)
                                                             cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:907642/143‑1 (MQ=255)
                                                             cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                              <  2:560303/143‑1 (MQ=255)
                                                                tgcacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTGCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACGCATCGCATCGCCTAAATGGAAAATTGGCAGATACACTGcc                                                                           <  2:25794/141‑1 (MQ=255)
                                                                accacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGACGTGGAGCAACACGCCAGCGTCATCCCATCCACCAGAAACCAGCGCCTAAACGGAAAATTGGCCGATCCATTGcc                                                                           >  2:779173/1‑143 (MQ=255)
                                                                     gTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccac                                                                      >  1:980725/1‑143 (MQ=255)
                                                                         aTTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGc                                                                  >  2:135545/1‑143 (MQ=255)
                                                                             gtgtGCAGGAACAAGACAACCGCAGCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTAc                                                              <  1:32254/143‑1 (MQ=255)
                                                                               gtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCAGAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCACCTAAATGGAAAATTGGCAGGTACATTGCCACCACCAGCGTACCa                                                            >  1:306819/1‑143 (MQ=255)
                                                                                 gCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaat                                                          >  2:954386/1‑143 (MQ=255)
                                                                                  cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAACGGAAAATTGGCAGATACATTGCCACCCCCCGCGTAGCaatc                                                         >  2:1028817/1‑142 (MQ=255)
                                                                                   aGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaataa                                                        <  1:847776/143‑1 (MQ=255)
                                                                                          aGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATtcctcc                                                 >  2:436124/1‑143 (MQ=255)
                                                                                           gAAAACCGCATCTTCAACAACCTGTAATCTCCCAGCGCTGGCGTGGAGCAACACGCCAGCGTTACCCCATCACACTCATCGCATCGCGTAAAGGGCAAATTGGCAGATGCATTGCCACCACCAGCGTACCACTAGTTCCTccc                                                >  1:132882/1‑143 (MQ=255)
                                                                                                  gCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAACTGGAAAATTGGCAGATACATTGCCACCACCAGCGTCCCCCTACTCCCCCCCGTTCTGa                                         >  1:762686/1‑143 (MQ=255)
                                                                                                   cATCTCCCACGACCTGGAATCTCCCCCCGCTGGCGTGGCGGAACGCGCCAGCGGTATCCCATCCCACTCGTCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGAt                                        <  2:1059237/143‑1 (MQ=255)
                                                                                                    aTCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATc                                       <  1:1000510/143‑1 (MQ=255)
                                                                                                     tCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                      <  1:565659/143‑1 (MQ=255)
                                                                                                         caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATAATCAGCaa                                  >  1:708219/1‑143 (MQ=255)
                                                                                                               cctgccaTCGCCCACCGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAAGTCCTCCCGTTATGATCAGCAACGCCgg                            <  2:736532/137‑1 (MQ=255)
                                                                                                                   taaccccccAGCGCCGGCGGGGAGCAACACGCCAGCGTTATCCCACCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATTCCTCCCGTTATGATCAGCAACGCCGGTTcc                        <  1:817795/137‑1 (MQ=255)
                                                                                                                         cccAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCCATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAg                  >  2:928376/1‑143 (MQ=255)
                                                                                                                           cAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGc                >  1:421744/1‑143 (MQ=255)
                                                                                                                                cTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCCGATACATTGCCACCCCCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGCCTGcaa           >  1:523063/1‑141 (MQ=255)
                                                                                                                                cTGGCGTGGCGCAACACGCCAGCGGTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGa           <  1:999031/143‑1 (MQ=255)
                                                                                                                                 tGGCCTGGACCCACCCGCCCGCGTTATCCCTTCCCACTCCTCGCATCGCCTAAATGGAAAATTGTCATATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAg          <  2:894302/143‑1 (MQ=255)
                                                                                                                                      tGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTAt     >  2:351277/1‑143 (MQ=255)
                                                                                                                                        gAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGGTCCAGTCAGGCTGCGAGGTCATcc   >  2:368998/1‑143 (MQ=255)
                                                                                                                                         aGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCGAAATGGAAAATTGGCAGATACATTGCCACCACCACCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTACGGCTTCGATGttcaccg  >  2:187422/1‑138 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCG  >  NC_000913/114370‑114649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑