| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 128.7 / ‑6.7 | 54 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (21/27); total (26/28) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTG > NC_000913/125248‑125522 | gaTCCTGGCGAAAGATGACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAgg < 1:418761/143‑1 (MQ=255) tCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGGGATGGTCAGGATTGTGAACGCTGGAACATGCTGAACCCGCTGGAAACTCCGCGCGTTCCGTAGACCGCTTAGGtg > 2:564479/1‑143 (MQ=255) cTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTACCGAATATCGCAAAGGagat > 1:572964/1‑143 (MQ=255) tGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGATGGCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCGGTATATCGGTTGGGtgatg > 1:990563/1‑143 (MQ=255) cGAAAGATTACTGCGTAGGTTCTGCCGTTTATAGCTTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac < 1:830932/143‑1 (MQ=255) cGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGCGATGAac > 2:923081/1‑143 (MQ=255) aaggacgaCGGTGCAGGTTCTGACGTTGATAGCGTGACCTCCTTCACCGCGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga < 1:812564/136‑1 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGATCCGTATGTCGCTCAGGTGacgaacga > 2:652048/1‑143 (MQ=255) aGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCGGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTATAGCGCTTGGGGGATGGacgacg > 2:112857/1‑143 (MQ=255) gTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCaat > 2:871820/1‑141 (MQ=255) gTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGt < 1:867134/143‑1 (MQ=255) tggcgACCTTTAGGGCGTGACTGCCTGGACCGCGCTGGCGCGTGGGCGGCAGAATGGTGAACGCTGGAACATGGTGCACCCTCTGGAAACGCGGCGCGTTCCGGATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAt < 2:924334/139‑1 (MQ=255) tGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCGCCCGCGGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGCTGAACGACGCTACGGCAGCGGCATCTa > 2:139403/1‑143 (MQ=255) gACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGAGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 1:203875/143‑1 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac > 2:953958/1‑119 (MQ=255) aCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac < 1:953958/119‑1 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGGCGCTCCGGCAGTGCCATCTACCGa > 2:696234/1‑143 (MQ=255) tttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACAAAGCCCTTTCCGTAGATCGCTGAGGCGATGAACGCCGCTCCGGCAGTGGCATCTACCGAc > 2:127617/1‑143 (MQ=255) ttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACt < 1:629842/143‑1 (MQ=255) ataGCGTGACCTCCTTCCCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtat < 2:65424/143‑1 (MQ=255) ctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCCGCAGTGGCATCTACCCACAATATGAAACTGt > 2:955815/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt < 1:767750/143‑1 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt > 1:174372/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAAGCTTATGTGCCGTTTATCATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATAGCGCTTAGGTGATGGACGACGCTCCGGCCGTGGCATCTACCGACTACATGAAACTGtt > 2:789539/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGCAACTCCGCGCGTTCCGTATATCGCGTAGGTGATGCACGACGCCCCCGCAGCGGCATCTACCGACTACATGAAACTGTCCg > 2:1072415/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATAGGAAGCTGTTCg > 2:585302/1‑143 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg > 1:491704/1‑143 (MQ=255) ccgcACCGGGCTGGCGGGTGCCTGTCAGGATCGTGCACGCTGGCACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 2:1012537/140‑1 (MQ=255) tCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCt < 2:409280/143‑1 (MQ=255) ccGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTAGCTGAg > 1:365811/1‑143 (MQ=255) cGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc < 2:910297/143‑1 (MQ=255) ccggCGCGTGATGGGCAGGATTGTGAACGCTGGCACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 1:122070/141‑1 (MQ=255) cTGGCGCGTGCTGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATCTCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 1:673843/143‑1 (MQ=255) cgcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGt > 1:512219/1‑143 (MQ=255) gcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGTTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTa < 1:696234/143‑1 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGCGATGAACGACGCTCCGGCAGTGGCATCTACCGACTCTATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAc > 2:390219/1‑143 (MQ=255) cGCTGGAACATGCTGCACCCGCTGGAGACTCCGCGCGTTCCGTATAGCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATCTGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGGCGACTa > 2:872223/1‑143 (MQ=255) ggAACATCCTGCCCCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATCAACGACGCTCCGGCAGTGGCATCTACGGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgc < 1:923081/143‑1 (MQ=255) gAACATGCTGCACCCGCTCGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg < 1:798013/143‑1 (MQ=255) ccATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACTTACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTa < 1:871820/142‑1 (MQ=255) ctgcACCCGCTGGAAGCGCCGCGCGTTCCGTCTATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTggg < 1:832266/143‑1 (MQ=255) tgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTggct > 1:232352/1‑141 (MQ=255) cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAc > 1:449668/1‑143 (MQ=255) ccGCTGGAAACTCCGCGCATTCCGTATATCGCTTAGGTGCTGAACGACGCGCCGGCAGTGGCATCTACCGACCATCTGAAACTGTTCGCTGAGCAGGGCCGTACTTACGTACCGGCCGGCGACTAGCGCGGACCGGCTCCTGa > 2:506742/1‑143 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATATCGCAAAGGTTATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 1:461858/143‑1 (MQ=255) gcaccTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCAGCTGACGACTACCGCGTACTGGGTACTGATGGCtt < 2:935599/139‑1 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTCATGAAAGGCGCGCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg < 1:750136/143‑1 (MQ=255) ttcgcgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTCGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc < 1:326741/141‑1 (MQ=255) tCCGCGCGTTCCGTGTATCGCTTAGTGTATGAACGACTCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc < 1:850054/143‑1 (MQ=255) gcgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGCCTACCGCGTCCTGGGTACTGATGGCTTCGGCCGtt > 1:590911/1‑143 (MQ=255) cgcgTCCCGTACATCGCTTAGGGGATGAACGACGCTCCGGCAGTGCCATCTACCGACTACATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc < 1:112857/143‑1 (MQ=255) gcgTTCCGTATATCGCTTAGGTGATGATCTACGCTCCGGCAGTGGAATCTACCGACTCTATGAAACTGTTCGCTGAGAAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTcc < 1:152362/143‑1 (MQ=255) tCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACa < 1:778224/143‑1 (MQ=255) tatCGCTTAGGTGATGAACGACGCTCCGGCCGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTg > 2:1082341/1‑143 (MQ=255) | GATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTG > NC_000913/125248‑125522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |