Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,101,541	G→C	11.4%	P28R (CCG→CGG)	yggN ←	putative EcfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,541	0	G	C	11.4%	158.1 / 11.5	58	P28R (CCG→CGG)	yggN	putative EcfF
Reads supporting (aligned to +/- strand):  ref base G (32/19);  new base C (5/2);  total (37/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

CCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT  >  NC_000913/3101401‑3101682                                                                                                                                             |                                                                                                                                              cgtgcGCCGGCTCGCGCGGGGCGGCAGGCGGGGAATATCGCTTACCGTTATACATCCCGTGGCCGTCGGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACgcggc                                                                                                                                             <  1:859477/139‑1 (MQ=255) ccTTCGCCTGCTCGCCCTGGCCGGCTTCGCTGGGATATTGCTTCCCGTTATACCTCACGTGGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACgcggc                                                                                                                                             <  1:856071/143‑1 (MQ=255)       cccgcgcgcgCTGGGCGGCAGTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACg                                                                                                                                       <  2:113940/137‑1 (MQ=255)       ccTTCTCCCGCTGGGCGGCAGTCAGGGAAGATTGCTTACCGTTCTACGTCACGTTGCCGTCTGGCGTGACCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACg                                                                                                                                       <  1:438359/143‑1 (MQ=255)        cTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGc                                                                                                                                      >  1:1018839/1‑143 (MQ=255)         tGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGCTTTCGATCACTTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACgat                                                                                                                                     <  2:780413/143‑3 (MQ=255)           cTCGCGCTGGGCGGCATTCGGGGAATATTGCTTACCGTTATCCATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCCGCGTGACGATgt                                                                                                                                   <  1:726688/143‑2 (MQ=255)           cTCGCGCTGGGCGGCATTCAGGGCATATTGCTTAACGTCATACATCACGTTGCCGACTGGCGTGAGCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGATCTGCGGGCCGACAGCAACACCGTCACACCGCGAGAccgcgc                                                                                                                                   >  2:139355/1‑138 (MQ=255)           cTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGc                                                                                                                                   >  1:1005204/1‑143 (MQ=255)            tCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGCTTGCCGTTTTCGCCCTTCACCTGCACGGTTCGCGGGCTGACAATCACATCCTCACGCGGCGCGACGcgccc                                                                                                                                  >  2:242295/1‑139 (MQ=255)                gcTGGGCGGCACTCAGGGAATATTGCTTACCGTTATCCATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCCGCACTgg                                                                                                                              <  2:592907/143‑1 (MQ=255)                    ggcggcATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCCCCTCCCCGGTTTGCGAGCTGACAATCACATCGCCACGCGGCGTGACGCTGCCCCGGTAGt                                                                                                                          >  2:216407/1‑143 (MQ=255)                      cggcATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCg                                                                                                                        >  1:433000/1‑143 (MQ=255)                          cttCAGGGACTATTGCTTCCCGTTATCCCTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCTCGCTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGt                                                                                                                    <  1:101989/142‑1 (MQ=255)                               gggAATATTGCTTACCGTTATACTTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCg                                                                                                               <  1:376182/143‑1 (MQ=255)                               gggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCCCATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGGCTGAGCg                                                                                                               >  1:335047/1‑143 (MQ=255)                               gggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTCGTCGCCGGCGGGACCg                                                                                                               >  2:23614/1‑143 (MQ=255)                                ggAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCgg                                                                                                              >  2:901362/1‑143 (MQ=255)                                     atctcTTCCCGTTATCCCTCCCGTTGCCGTCTGGCGTGATCCCCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACACTCATATCCTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAtt                                                                                                         <  1:758202/139‑1 (MQ=255)                                      ttGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTCCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTg                                                                                                        >  1:99711/1‑143 (MQ=255)                                              cGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGCGCGGTCATTGCCGTCACt                                                                                                >  2:167935/1‑143 (MQ=255)                                                ttATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCCGTCATTGCAGTCCCTGa                                                                                              >  1:1089934/1‑143 (MQ=255)                                                  ataCATCACGTTGCCGTCCGGCGTGATCCCCTGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                            <  2:867057/143‑1 (MQ=255)                                                  ataCATCACGTCGCCGTCTGGCGTGATCACCAGATTGCCGTCTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa                                                                                            <  1:676473/143‑1 (MQ=255)                                                      aTCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgc                                                                                        >  1:1040658/1‑143 (MQ=255)                                                        cACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCCGTCATTGCCGACAATGAAAGtgctg                                                                                      >  2:168796/1‑143 (MQ=255)                                                        cACGTTGCCGTCTGGCGTGATCACCAGATTCCCGCTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCACTGCCGTCACTGAAAGtgctg                                                                                      >  2:673512/1‑143 (MQ=255)                                                                cGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGCGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTCGTCGGCGTGAGCGGCCATCGCCGTCACTGCAAGGACTGCCCCcagc                                                                              >  2:331068/1‑143 (MQ=255)                                                                 gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCTCATCGGCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCcagca                                                                             <  2:1095388/143‑1 (MQ=255)                                                                 gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTTGTCGGCGTGAGCGGTCATTGCCGTCACTGGAATTGCTGCCGCcagca                                                                             >  1:782127/1‑143 (MQ=255)                                                                        gTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCAgttt                                                                      >  2:51547/1‑143 (MQ=255)                                                                          gATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTgc                                                                    <  1:319557/143‑1 (MQ=255)                                                                              aCCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGcat                                                                >  2:1074646/1‑143 (MQ=255)                                                                               ccAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGAGcctc                                                               >  2:1056734/1‑143 (MQ=255)                                                                                caagttGCCGCTCTCGCCCTTCACCGGCACGGGTGGCGGGCTGACAATCACATCGCCACGCGGCGTGACGTTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGcatca                                                              <  1:56852/139‑1 (MQ=255)                                                                                        cGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTCTGCGCATCATAGTCTTc                                                      >  1:782949/1‑143 (MQ=255)                                                                                         gTTTTCGCCCTCCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTcc                                                     <  1:464602/143‑1 (MQ=255)                                                                                         gTTTTCGCCCCTCGCCTGCACGGTTTGCGGGCTGACAGTCACGTCGTCACGCGGCGTGACGCGGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTcc                                                     <  1:357297/143‑1 (MQ=255)                                                                                          tggtCGCCCTTCACCGGCACGGTTTGCGGGCTGACAATCACATCGCCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTccc                                                    <  1:627787/140‑1 (MQ=255)                                                                                                cccTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTAACGCGACGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCGGTCACTGAAAGTGCTGCCGCCAGCAGCAATGTGCGGATCATGGACTGCCCTCAAGa                                              >  2:86274/1‑143 (MQ=255)                                                                                                   ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCAGTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATGTTGCGCATCATAGTCTGCCCGCAAGaaaa                                           >  2:853252/1‑143 (MQ=255)                                                                                                    tCACCGGCACGGTTTGCGGGCTGACAATCGCACCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaa                                          <  1:473350/143‑1 (MQ=255)                                                                                                           cACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                   >  1:1012732/1‑143 (MQ=255)                                                                                                             cGGTTGTCGGGCGGACACTGCCATCGTCACGCGGCGGGACGCTGCGCTCGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCa                                 <  1:126280/143‑1 (MQ=255)                                                                                                                tttGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                              >  1:836537/1‑143 (MQ=255)                                                                                                                    cGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCGGGGTTAGTCGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGGCGGCGCCGGCGGGGGATTGCGCATCAGAGTCTTCCCGCCACAAGAAAGCAGCCCACCAtttc                          >  2:20746/1‑142 (MQ=255)                                                                                                                         gggcAATCACATCGGCACGGGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                     <  2:811049/140‑1 (MQ=255)                                                                                                                         ggACAAGCACATCGTCACGCGGCGTGACGCTGCGCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                     <  2:868626/142‑1 (MQ=255)                                                                                                                             aaTCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGCAAGTGCTGCCGCCAGCAGCCTTTCGCGCATCATCGTCTCCCCTCAAGAAAAAATCAGGCCAGCATATGCTGGcccaa                 >  1:39183/1‑141 (MQ=255)                                                                                                                               tCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCGTAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATATGCTGGCCCCAGa               >  1:613174/1‑143 (MQ=255)                                                                                                                                cacaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt              <  2:1040658/143‑1 (MQ=255)                                                                                                                                cacaTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGACCTCCCTCCTGAATATATCAGGCCAGCATTTGCTGGCCCCAGAt              >  1:750014/1‑143 (MQ=255)                                                                                                                                  cagcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGCCCCCAGCTTg            >  2:349857/4‑143 (MQ=255)                                                                                                                                  caTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg            >  1:539055/1‑143 (MQ=255)                                                                                                                                  caTCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg            >  1:17578/1‑143 (MQ=255)                                                                                                                                   agcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCTTCAAGAAAAAATCAGGCCAGCATTTGCTGGACCCAGATTGa           >  1:527780/3‑143 (MQ=255)                                                                                                                                   agcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa           >  2:512466/3‑143 (MQ=255)                                                                                                                                    gcGTCACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCCGCATCAGGTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGGTTGAt          >  1:929499/2‑143 (MQ=255)                                                                                                                                       tCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATGAGGCGAGCACTTGCTGGCCCCAGATTGATAAc       >  2:1066213/1‑143 (MQ=255)                                                                                                                                       tCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCAGACACCAGATTGATAAc       >  1:146568/1‑143 (MQ=255)                                                                                                                                          cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa    <  1:306603/143‑1 (MQ=255)                                                                                                                                           gcggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAACAAATAATTCCTGCATTTGCTGGCCCCCGATTGATAACAAAg   >  2:486484/1‑143 (MQ=255)                                                                                                                                           gcggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGGTTGATAACAAAg   >  1:754158/1‑143 (MQ=255)                                                                                                                                           gcggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGACCCAAAATGGATAACacaa   >  2:253589/1‑140 (MQ=255)                                                                                                                                           gcggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGCCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg   >  1:813401/1‑143 (MQ=255)                                                                                                                                            cggcgTGACGCTGCCCTGGTAGCCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt  <  1:729158/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                              CCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT  >  NC_000913/3101401‑3101682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑