Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,184,219 A→G 7.5% M83T (ATG→ACG)  ribB ← 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,184,2190AG 7.5% 169.3 / 2.5 63M83T (ATG→ACG) ribB3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (2/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGG  >  NC_000913/3184080‑3184359
                                                                                                                                           |                                                                                                                                            
tGACGGTTTTGCGCCATCGGCAATCGCCGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACcatca                                                                                                                                           >  1:899343/1‑143 (MQ=255)
  aCGGTTTTGCGCCCTCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCAtt                                                                                                                                         <  1:843084/143‑1 (MQ=255)
  aCGGCTTTGCGCCCGCGGCAACCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCAtt                                                                                                                                         <  1:129995/143‑1 (MQ=255)
    ggTTTTTCGCCCTCGGCAATCGCGGCGCGGACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCCGCTGCTTCTATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTgg                                                                                                                                       <  2:43082/143‑1 (MQ=255)
                   gCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTAAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTCGGTTCTAGCATCATTGGCAGAGCGAGGTGttt                                                                                                                        >  2:486065/1‑143 (MQ=255)
                       gcgcgGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACgg                                                                                                                    <  1:226168/142‑1 (MQ=255)
                         gCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCg                                                                                                                  >  1:738021/1‑143 (MQ=255)
                              gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCGTCATTGGCAGATCGAGTTGTTTACGGCGATCtt                                                                                                             >  1:591886/1‑143 (MQ=255)
                              gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCGTCATTGGCAGATCGAGTTGTTTACGGCGATCtt                                                                                                             >  1:4151/1‑143 (MQ=255)
                                gAACGGTCGTAATACGGTCAGTGGCATAAACACCGGTAGTCACACCTTAAGGTGCTACAAGGGGCAGGGGAAAACCGAGGCGAGAGGCGCTGGGGGGAGGGTCGACCATCATGGGAAGAGCGAGTTGTGTACGGCGATCTGCa                                                                                                           <  1:1080680/143‑1 (MQ=255)
                                gAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCa                                                                                                           <  1:991417/143‑1 (MQ=255)
                                   cGGTCGTAATACCGTCAGCGGCAGAAACCCCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTa                                                                                                        <  1:249786/143‑1 (MQ=255)
                                     gTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCaataat                                                                                                      >  1:573311/1‑143 (MQ=255)
                                           aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAg                                                                                                <  2:37015/143‑1 (MQ=255)
                                           aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAg                                                                                                <  1:1044488/143‑1 (MQ=255)
                                            tACGGTCAGCGGCAGAAACACCGGTAGGCACACCTTCAGCTGCTTCAATGGTCCCGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAgg                                                                                               <  1:10674/143‑1 (MQ=255)
                                               ggTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGACCTTCAGTAATGCACAGGCaa                                                                                            >  2:1088506/1‑143 (MQ=255)
                                                gTCAGCGGCAGAAACACCGGTAGTCACACCTTGAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTCACGGCGATCTTCAGTAATGCACAGGCaaa                                                                                           >  2:581157/1‑143 (MQ=255)
                                                gTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCaaa                                                                                           <  1:767644/143‑1 (MQ=255)
                                                     cGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGCTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATa                                                                                      >  1:868804/1‑143 (MQ=255)
                                                           aaaCACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTa                                                                                <  1:530916/143‑1 (MQ=255)
                                                             acacCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCGAACAATCCCGCTAcc                                                                              >  2:356392/1‑143 (MQ=255)
                                                               acCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGCGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACAGt                                                                            >  1:366824/1‑143 (MQ=255)
                                                                ccGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTg                                                                           <  1:877428/143‑1 (MQ=255)
                                                                 cGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTgg                                                                          <  2:726200/143‑1 (MQ=255)
                                                                  ggTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGc                                                                         <  2:738021/143‑1 (MQ=255)
                                                                       tCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATg                                                                    <  1:932922/143‑1 (MQ=255)
                                                                        cacaCCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCCTAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTAACGTAGACAGCGCCAGACCATACCGCTACCGTGGCGAATgg                                                                   >  2:778519/1‑143 (MQ=255)
                                                                         acacCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGAAGGATTAACCATCGTCGTTCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGt                                                                  >  1:253478/1‑143 (MQ=255)
                                                                         acacCTTCAGCTGATTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGACTGGt                                                                  >  2:1105127/1‑143 (MQ=255)
                                                                          cacCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTc                                                                 >  1:763084/1‑143 (MQ=255)
                                                                               tccgctgctTCAATGGTCACGATAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTCCCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAgcgc                                                            <  2:994855/140‑1 (MQ=255)
                                                                                   ctgctTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCCCAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATc                                                        >  1:843432/1‑143 (MQ=255)
                                                                                     gctTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTGATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTg                                                      <  1:568737/143‑1 (MQ=255)
                                                                                       ttCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCCTCCTTGGCAGATCGAGTTCTTTACGGCGATCTTCAGTAATGCACAAGCAAACAATACCGCTTCCGTGGCCAATCCTCAGCGCCacccgcc                                                    >  1:1027745/1‑137 (MQ=255)
                                                                                        tCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACAATAATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTACTGCCACGGCAAGCAATACCGGTACAGTGGCGATTGGTCAGCGCCATCTGCTc                                                   >  1:410399/1‑143 (MQ=255)
                                                                                            tGGTCACGGTAACACCGGTGCCATAGGCGCTGGTGTCATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACa                                               <  2:560575/143‑1 (MQ=255)
                                                                                                 aCGGTGAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGCAGGGTCAGCGCCATCTGCTCAACAGTCAt                                          >  1:124546/1‑143 (MQ=255)
                                                                                                 aCGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGACTGGTCAGCGCCATCTGCTCAACAGTCAt                                          >  2:860661/1‑143 (MQ=255)
                                                                                                 aCGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCAt                                          <  1:793561/143‑1 (MQ=255)
                                                                                                    gTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGt                                       >  1:1004910/1‑143 (MQ=255)
                                                                                                      aaaaACGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGttt                                     <  1:738453/143‑1 (MQ=255)
                                                                                                            ggTGCCATAGGCGCGGGTGGTATTTGCTACCGTCAGTGGCAGATCGAGTTGTTTACGGCGATCTTCAGGAATGCACAGGCAAACAAGACCGCGACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCg                               <  2:811923/143‑1 (MQ=255)
                                                                                                             gTGCCGTAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCgg                              <  1:52951/143‑1 (MQ=255)
                                                                                                             gTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACGATACTGCTACCCTGGCAAATCGTCAGCGCCATCTGCTCAACAGTCATGGGGTCTGCCgg                              >  1:804308/1‑143 (MQ=255)
                                                                                                                ccATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGaa                           <  1:164406/143‑1 (MQ=255)
                                                                                                                    aGGCGCTGGTGTTATTTTCTACCGTCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATc                       <  2:591886/143‑1 (MQ=255)
                                                                                                                    aGGCGCTGGTGTTATTTTCTACCGTCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATc                       <  2:4151/143‑1 (MQ=255)
                                                                                                                     ggCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACCGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCGCAACAGTCGTGGTTTCTGCCGGGAAGATCa                      >  1:337123/1‑143 (MQ=255)
                                                                                                                      gcgcTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCat                     >  1:255788/1‑143 (MQ=255)
                                                                                                                      gcgcTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCat                     <  1:314159/143‑1 (MQ=255)
                                                                                                                      gcgcTGGTGTTATTTTCTACCATCATTGGCAGACCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCACCAGTCATGGTTTCTGCCGGGAAGATCat                     >  2:645927/1‑143 (MQ=255)
                                                                                                                         cTGGGGTTATTTTCTACCATCTTTGGCAGGTCGAGGTGTTCACGGCGATGTTCGGTAACGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATc                  <  2:957311/143‑1 (MQ=255)
                                                                                                                              gtTATTTTCCACCATCATGGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCtt             <  1:108482/143‑1 (MQ=255)
                                                                                                                                 cttttCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGt          <  2:364450/142‑1 (MQ=255)
                                                                                                                                 aTTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGt          >  1:1059432/1‑143 (MQ=255)
                                                                                                                                 aTTTTCCACCATCATTGCCAGATCGAGTTGTTTACGGCGATCGTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGt          <  1:321217/143‑1 (MQ=255)
                                                                                                                                   tttCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCg                                                                                   >  2:43558/1‑68 (MQ=255)
                                                                                                                                   tttCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCg                                                                                   <  1:43558/68‑1 (MQ=255)
                                                                                                                                    ttCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACCGTCATGGTTTCTGCCGGGCAGATCATATCCCCCTCGtttt       >  1:27199/1‑143 (MQ=255)
                                                                                                                                     tCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACCGTCATCCCCGCGGCCGGGAAGCTCATATCACCTTCGTTTTc      >  1:1041538/1‑143 (MQ=255)
                                                                                                                                     tCTACCATCAGTGGCAGATCGAGTTGCTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTc      <  1:500947/143‑1 (MQ=255)
                                                                                                                                       tACCATCATGGGCAGATCGAGGTGTTCACGGCGAGCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCAc    <  1:1017832/143‑1 (MQ=255)
                                                                                                                                         ccATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACgg  >  1:804028/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATCTTCAGTAATGCACAGGCAAACAATACCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGG  >  NC_000913/3184080‑3184359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑