| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,352,184 | C→G | 41.0% | R281P (CGC→CCC) | arcB ← | sensory histidine kinase ArcB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,352,184 | 0 | C | G | 41.0% | 20.1 / 48.2 | 49 | R281P (CGC→CCC) | arcB | sensory histidine kinase ArcB |
| Reads supporting (aligned to +/- strand): ref base C (13/15); new base G (5/15); total (19/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
GCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGCCCGTCC > NC_000913/3352043‑3352321 | gCCGGAACATGGATGGTCTTGAGGTATCATTCGGTGTTGGCGGTGAGTGCGGTATCCAGCAGAATGCGTCTCAGACCGACTATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTcgcg < 2:1069177/143‑1 (MQ=255) aaCGTGGATGGTCTTGCGATAGTCTTCCTGCTCGGCGGTGAGTGCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTgg < 2:449785/143‑1 (MQ=255) aaCATGAATGGTCTTGAGATATTTTTCCTGCTTGGCGGTGAGTTCGGTATCCAGTAGAATGCGGCTCAGACTGACGTTATCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTgg < 1:425938/143‑1 (MQ=255) aCATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGcggccggc < 1:698323/143‑1 (MQ=255) aCATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCCcggccggc > 1:739576/1‑143 (MQ=255) cgtggctggTCTTGAGATATTTTGCCTGCTCGGCGGTGCGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGcc < 2:170116/141‑1 (MQ=255) atggatggTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGccc > 2:476399/1‑143 (MQ=255) ggatggTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGt < 1:688066/143‑1 (MQ=255) ggatggTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCCGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCAGTGTACGCAATTCGTGACTGATGGTGGAGATCAACCGCGTTTTGTCGGGGCTGGCCCgc > 2:989892/1‑142 (MQ=255) tCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGCTAAACGTCGTTTTGTCGCGGCTAGCCCGTTCAAgc > 1:387509/1‑143 (MQ=255) ttGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAgcgc > 1:349795/1‑143 (MQ=255) tttCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATAc < 1:393183/143‑1 (MQ=255) tttCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGCCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGCTGGTGGAGATAAACGGCGGTTTGTCGCGGCTGGCCCGTCGAAGCGCATCCTggtcc > 1:284360/1‑139 (MQ=255) cggTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGATCCGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGGATGGTCAAAGCTGGCTCGTTCAAGCGCATCCTGATACCGCGTACGCTCg > 2:488324/1‑143 (MQ=255) gttGAGTGCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCgg < 1:913392/141‑1 (MQ=255) aGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGTCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAAt < 1:587959/143‑1 (MQ=255) aGTCCGGTAGCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGACATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAAt < 1:188132/143‑1 (MQ=255) tCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTc < 1:476399/143‑1 (MQ=255) gAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGccc < 1:629679/143‑1 (MQ=255) taTGCGGCTCAGGCCGACGATACCGTTCAGTTGTGCACGCGTTTCGTGACTGATGGTGGAGATACACGTCGTTTTGTCCCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCa < 2:150212/142‑1 (MQ=255) tGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATc > 2:867756/1‑143 (MQ=255) ggcgcggACCGACGATGCCGCTCGGCGGTGGACGGAATTCGTGGCTGATGGGGGAGATAATCGTCGTTTTGTCGCGGTTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCaaa < 1:337762/137‑1 (MQ=255) gCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGCTACCGCTTACGCTCGGTGATGTCGCGACCACAGCCCATCAAAc > 2:22369/1‑143 (MQ=255) gCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAAc > 1:299923/1‑143 (MQ=255) tCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAGACCg > 1:618182/1‑143 (MQ=255) cAGACCGGCGATACCGGTCAGCGGTGTACGCAATTCGTGACTGATGGGGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGt < 2:245881/143‑1 (MQ=255) cAGACCGACGCTACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGt < 2:1006531/143‑1 (MQ=255) gACCGTCGATACCGTGCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGa < 2:489663/143‑1 (MQ=255) gACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCAGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCACACCGtaa > 1:550050/1‑141 (MQ=255) aCCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGATGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGAGCGAGGCCCAGCACACCGTGAc > 1:71945/1‑143 (MQ=255) ccGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGACTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACg < 1:848797/143‑1 (MQ=255) cgaCACCTTTCAGCGGCGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTa < 1:273424/143‑1 (MQ=255) gaTACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTAc > 2:370909/1‑143 (MQ=255) aTACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAATCGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCTCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTAcc < 1:221142/143‑1 (MQ=255) tACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTAccc > 1:758444/1‑143 (MQ=255) ttCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCACGTTCAAGCGCATCCTGATACCGCTGACGCTCGGTCATGTCGCGCCCGAAGCCCAGCAAACCGTGACGTTTACCCAggcc > 2:325195/1‑142 (MQ=255) ggTGTCCGCAATTCGTGCCTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCAGCCTGACACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCgta < 1:209399/143‑1 (MQ=255) cAATGCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGGGCCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCa < 2:366560/143‑1 (MQ=255) aTTCGTGACTGATGGTGGAGCTAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACt < 2:349795/143‑1 (MQ=255) ttCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACtt < 1:901918/143‑1 (MQ=255) cTGATGGTGGAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGAt < 1:867756/143‑1 (MQ=255) tggtggAGTTAAACGTCGTTTTGGCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCa < 2:258253/143‑1 (MQ=255) tggAGATAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAg < 1:758135/143‑1 (MQ=255) gaggTAAACGTCGTTTTGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCa < 1:343690/143‑1 (MQ=255) agaTAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGCCGTTTACCCACGCGGTCGTAGCACGGCACCTTACGGCTTTCAAAGCAg > 2:743548/1‑143 (MQ=255) gttAAACGTCGTTTCGTCGGGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAgg < 2:1003956/141‑1 (MQ=255) cgtggtTTGGTCGGGGCGGGGCCGGGCAAGCGCAACCTGAGACCGCTGACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTgc < 1:32886/143‑1 (MQ=255) cgtcgtGTCGTCGGCGCTGGCGCGTCCAAGCGCCACCCGATACCGCCTACGCTCCGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTGACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTgc < 1:586816/143‑1 (MQ=255) ctttgGTGGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGccc < 2:618182/142‑1 (MQ=255) ttttGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTCCCCACGCGGTCGTAGTCCGGCACTTTACGGATTTCAAAGCAGGCGTTGCGCCCg > 1:963137/1‑143 (MQ=255) ttGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCACTCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGCCCGTc > 2:69493/1‑143 (MQ=255) tGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACCGATTTCAAAGCAGGCTTTGCGCCCGTcc > 1:1001185/1‑143 (MQ=255) | GCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGCCCGTCC > NC_000913/3352043‑3352321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |