| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 419,671 | Δ1 bp | coding (81/1320 nt) | brnQ → | branched chain amino acid transporter BrnQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 419,670 | 0 | T | . | 91.7% | 81.6 / 0.8 | 24 | coding (80/1320 nt) | brnQ | branched chain amino acid transporter BrnQ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (7/15); minor base G (2/0); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTG > NC_000913/419539‑419798 | aTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTcc > 1:685966/1‑143 (MQ=255) gCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATg > 1:505952/1‑143 (MQ=255) gCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑CACTGTCCCTCCAATg > 2:248065/1‑143 (MQ=255) aaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGGAACA‑GCTTTTCCCTCCAGTGGCCGGc > 2:485662/1‑143 (MQ=255) acaccaCCATAGCCGCAGGCAGTTCTGTGTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTTGGCTTTACGCCATTCTCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACaggcaggc < 2:123158/143‑1 (MQ=255) aacaCCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGCTATCATCGCTTTGGGCTTTATGACCCTCGCGTTGTTCGCCGGCGCAGGTAAC‑GAATTTTGCCTCCAATGGTCCGCTTCCAGGCCGGCGa > 2:230373/1‑143 (MQ=255) aTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCt < 2:773742/143‑1 (MQ=255) cacaGGCAGTATGATTTATCACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGa < 2:149076/143‑1 (MQ=255) acaGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACCGGCCGGCGAAACCGTCTggtc > 1:284625/1‑141 (MQ=255) aTGATTTATGACCCGTCAATTCAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCATGCGAACACGTCTGGACTGCGGCAtt < 2:322319/143‑1 (MQ=255) aTCAATTAAGTTCGCGCGCTATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCATTGGGCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTa < 1:179846/143‑1 (MQ=255) gATCGCGCGCTATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTg < 1:159474/143‑1 (MQ=255) cgctatCACCGCTCTGGTCTTTAAGACCCTTTCTTGGTTCATCGGCGCAGGTAACA‑GATTTTCCCTCCATTCGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGcc < 2:585635/140‑1 (MQ=255) atcagcGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTa < 1:563816/143‑1 (MQ=255) atcGCTCTGGGCTTTATGACATTTGAGTTGTTCGGTATTGCAGTTTTCAGT‑TTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTa > 1:537069/1‑143 (MQ=255) tttATGACATTTGCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTCTTCACGGTGGTGgcg > 1:102301/1‑143 (MQ=255) aTGCCATTTGCGTTGGTCGTCGGCGCAGGTAACA‑TATTCTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATCACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTg < 1:611651/143‑1 (MQ=255) tttGCGTTGTTCGTCGGCGCAGGTAACA‑TATATTCCGTTCAAGCGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCaaaa < 1:606604/143‑1 (MQ=255) gttGCGTTGTTCGTCGGCGCAGGTAACA‑TACTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGTCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCaaaa < 1:337737/142‑1 (MQ=255) gCGTTGTTCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCCGCTTACAGGCAGGCGAACACGTCTGGGCTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGtt < 1:57770/143‑1 (MQ=255) gttgttCGGCCGCGCAGGTAACA‑GATGGCCCCTCCGAGGGTCGGCTTACAGGCAGGCGAACACGGCTGGACTGCGGCATTCGGCTTCGTCATTGCTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTgg < 2:439299/143‑1 (MQ=255) ttgttCGTCGGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTggc < 1:612898/143‑1 (MQ=255) tcgtcgGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGgcggcg < 1:159412/143‑1 (MQ=255) tcgGCGCAGGTAACA‑TATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGGTGGCCTGCCGGTATTAACGGTAGTGGCGCGGGCAAAAGTTGGCGGCGgtg > 1:606865/1‑143 (MQ=255) | ATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTGGCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTG > NC_000913/419539‑419798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |