| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 786,462 | (A)5→4 | coding (830/1053 nt) | aroG → | 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 786,458 | 0 | A | . | 97.1% | 129.5 / 0.1 | 36 | coding (826/1053 nt) | aroG | 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (18/15); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
CCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGT > NC_000913/786319‑786595 | ccATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCGCGTGGCTGAAGTGAAAGAAGGGCCGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaa < 1:356505/143‑1 (MQ=255)ccATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaa < 1:310664/143‑1 (MQ=255) agagcATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaagc < 2:492188/139‑3 (MQ=255) atCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAAAAGCACTTTAAGGGCAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaagcag > 1:570450/1‑139 (MQ=255) cATTCTGCGCGTCGGTAAGGAGCCTTACTACGGCACGAAGCACGTTGCTGAAGTGAAAGAAGGGTTGAACATAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAgat < 2:572525/143‑1 (MQ=255) ttCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAgatgg < 1:2984/143‑1 (MQ=255) gcggcgGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGttt < 1:236587/143‑1 (MQ=255) cggTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGttt < 1:400692/139‑1 (MQ=255) cggTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGttt > 2:400692/1‑139 (MQ=255) aaaGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAA‑CAGATGGGTGTTTGTGCTgcc > 1:457058/1‑141 (MQ=255) agagCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGTAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACACCTC‑CAAAGCCGATGGATGCTTGTGCTGACGt > 2:731819/1‑143 (MQ=255) ggtgaaCGACAGCGCGAAGGACGTCGCTGGAGTGAAAGGAGTGCTGAACCAAGCAGGCCTGCCAGGACCGGCGATGGTCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTg < 2:348051/139‑1 (MQ=255) ccTAACTACAGCGCGAAGCACGTTGCTGAAGTGACAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACACGTCCAAACAATTC‑CAAACCAGAGGGATGTTTGTGCTGACGCTTGc > 2:25514/1‑143 (MQ=255) accTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCcag < 1:758508/141‑1 (MQ=255) tACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTCACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGCCGTTTGCcagcag > 2:178150/1‑143 (MQ=255) ggcAGCGCGAAGCACGTGCCTGGACGGATAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCTACCAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCcagcag < 2:228110/141‑1 (MQ=255) cAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGCACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑CAAAGCAGATGGACGTTTGTGCTGACGTTTCCccgcagct > 2:171495/1‑141 (MQ=255) gAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCgg > 1:734282/1‑143 (MQ=255) tGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑ACAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCatta > 2:361793/1‑143 (MQ=255) aaaGAAGGGCTGAACAAAGCAGGCATGCAAGCACGGGGGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATGGCCGGGGGCGAAAAGGCCatgatt > 1:403832/1‑143 (MQ=255) agGGCTGAACAAAGCAGGCCGGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGt < 1:460054/143‑1 (MQ=255) tGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGAGGCGAAAAGGCCATTATTGGCGTGAtgg > 2:449501/1‑143 (MQ=255) aaCAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAtggtg > 1:464238/1‑143 (MQ=255) aaaGCAGGCCTGCCAGCACAGGTGATGGTCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGaa < 1:153031/143‑1 (MQ=255) aGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACCCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAGGGTGGaatg > 1:497855/1‑141 (MQ=255) gCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAGGCCATc > 2:688512/1‑143 (MQ=255) gCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATc < 1:401393/143‑1 (MQ=255) tGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCAATTATTGGCGTGATGGTGGAACGCCCTCtgg > 1:335738/1‑143 (MQ=255) gccacgacaGGTGATGATCGCTCCCAGCCATGCTAACTCGTGAAAACAGTT‑GAAAAGCAGATCGATGTTCGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCtggt < 1:212133/137‑1 (MQ=255) tgatCGATTTCAGCCATGCTAACTCGTCCAAACAATTC‑GAAAGCAGATGGATGTTTGTGCTGACGTGTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATGATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCaga < 2:759732/143‑1 (MQ=255) tCGATTTCAGCCATGCTAACTCGTCCAAAAAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGAAGGTGGAAAGCCATCTGGCGGACGGCAATCAGAGcc > 2:683902/1‑143 (MQ=255) gATTTCAGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTc < 1:515816/143‑1 (MQ=255) aGCCATGCTAACTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCGGGTGGAAGGCAATCAGAGCCTAgagacc > 1:502461/1‑141 (MQ=255) gCTAACTCGTCCAAACAATTC‑AATAGCAGATGGATGTTTGTGCTGACGTTTGCCAGGAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAg < 1:734696/143‑1 (MQ=255) aaCTCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCg < 1:43339/143‑1 (MQ=255) tCGTGCAAGCACTTC‑AAAAGAAGATGGATGTTGGTGCTGACGTTTGCCAGCAGATGGCCAGTCGCTAAAGTTCCATTCTGGGCGTGATGGTGGGAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTg < 2:192823/143‑1 (MQ=255) tCGTCCAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTg > 2:431895/1‑143 (MQ=255) gccAAACAATTCTGG‑AGCAGATGGATGTGTGTGCTGATGTTTGCCACAAGATCGCCTGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGGAAGCCAGTTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGcc < 2:88562/142‑1 (MQ=255) ccAAACAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGACATTATTGGCGTGATGGTGG‑AAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTa > 1:267976/1‑143 (MQ=255) cAATTC‑AAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGCAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGGGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCAGGCCTaagag > 2:81542/1‑139 (MQ=255) | CCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGT > NC_000913/786319‑786595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |