| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,637,646 | +T | coding (5/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 95.1% | 117.7 / 0.2 | 41 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (14/25); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
AATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATC > NC_000913/3637504‑3637770 | aaTTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑t > 1:712551/1‑143 (MQ=255) atataAGATAATTATTTTTTGAGTGAAATACCTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑ttaca < 2:257384/143‑5 (MQ=255) aGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACAtttgt < 2:354627/143‑1 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCACAATGGCGTAACGTCCTATG‑CTT‑ACAtttgtt > 1:82737/1‑143 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACAtttgtt > 1:137499/1‑143 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACAtttgtt > 1:137496/1‑143 (MQ=255) tAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACAtttgtttg < 1:28972/143‑1 (MQ=255) aaTTATTTTTTGAGTAAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGc > 2:660687/1‑143 (MQ=255) ttattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTg > 1:182895/1‑143 (MQ=255) attTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGGCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGc > 1:291719/1‑143 (MQ=255) gcgtgtAATCCAGACAGGGGGCACAGCCAAAAACGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTAGCATGGAtt < 2:729091/141‑1 (MQ=255) attGAAACTCATACAGGGCGCAAAACAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGAttt < 2:655470/141‑1 (MQ=255) gTGAAAGCCATACAGGGGGCAAATCAAAAAAAGTCTATATTGCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTg < 1:538012/143‑1 (MQ=255) tGAAATCCATACAGGGGGCCAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGACAATGATACTGCGCCGCGTTCATGTCCTCCAAAGGACGTAACGTCCGATG‑CTT‑CCATTTCATCACAGGCCTGGCTTTGc > 2:23794/1‑143 (MQ=255) tCCATACAGGGGGCAAATCAACAAAAGTCTCTTTTTCATTTTGCGCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAAGGTCCTATG‑CTT‑ACATTTGTTTGCTGGCATGGATTTGCATAcc > 2:684480/1‑143 (MQ=255) gggCAGACCGAAAAAAGTCCTGATTTCACGTTGCCCGCGGCGCGAAAGTGACTGAGAATGCGCGGCGTGCGTGCCCTCAAGCTGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCCTACCGGGCTGttat < 1:589848/143‑1 (MQ=255) tCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTACTATGAT‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGATGTTATTAtttct > 1:628949/1‑143 (MQ=255) aGTCGATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTgg < 1:606917/143‑1 (MQ=255) atTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGTATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgt < 1:189712/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtctg > 1:714064/1‑143 (MQ=255) tttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTc < 2:572339/143‑1 (MQ=255) tGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACCTTGGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTa < 1:491493/143‑1 (MQ=255) ctggccgcgAAAGTCGCTGATAATGCGCCGCCTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TCACATTTGTCTGCTGGCCTGGAGTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 2:766980/140‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 2:182895/143‑1 (MQ=255) gcgcctcgAAAGTCACTGATAACTCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAg < 2:33208/143‑1 (MQ=255) gcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAg < 2:622021/143‑1 (MQ=255) gcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAGGCCacc > 2:257737/1‑141 (MQ=255) aaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAAt < 1:555946/143‑1 (MQ=255) aaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATg < 1:77385/143‑1 (MQ=255) aaGTCACTGATAACCCGCCGCGTTCCTCTCCTCAAAACGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATg < 1:43885/143‑1 (MQ=255) tCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTTCTTGCACTGGCTTTTGTGCTGTTCTACGAACCCATCCATTGtt > 1:455150/1‑143 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 1:357351/143‑1 (MQ=255) tactGCGCGCCGTTCAGTTCCTCAAAATGGCGTATCGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGTCGGGTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 2:251264/140‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:660687/143‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 2:243264/143‑1 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:312059/143‑1 (MQ=255) aaTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCACTGGTTTCCATGac > 2:607722/1‑143 (MQ=255) gccTCAAAATGGCGTAACGTCCTATG‑CTT‑GTATTTATATGCCTGCCTGGATTTGCATACCTGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGc < 2:549552/142‑1 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTCCGAAGCCATCAATGGTTTCCCTGACGCAGCCAACGCCGTGGcga > 1:619194/1‑141 (MQ=255) tCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGCAGCCATCAATGGTTTCCATGAAACAGCCAACGCCGTAGCAAc > 2:649675/1‑143 (MQ=255) aaTGGCGTAAAGTTCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGtt < 2:43871/143‑1 (MQ=255) agggCGTACCGTCCTATG‑CTT‑ACATTTGTGTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTa < 2:646267/141‑1 (MQ=255) ggggTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGCATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATc < 1:397000/140‑1 (MQ=255) | AATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATC > NC_000913/3637504‑3637770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |