| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 97.4% | 108.4 / NA | 40 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (20/18); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA > NC_000913/125258‑125525 | tgaGATTGCGGCGTAGGTGCTGACGTGTATAGCGTGACCGCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga < 2:369122/141‑1 (MQ=255) aGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACATCCTTCACCGACCTCGCGCGGGTGGGTCGGGAAAGAGAACGCCGGAACATGCTGCACCCGCCGGAAACTCCGCGCGGCCCGTATAGCGCTCAGGCGATCAacgagc > 2:555118/1‑141 (MQ=255) ttACGGCGTAGGTTCTGACGTTTATAGCGTGAACTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCGGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTc > 1:11935/1‑143 (MQ=255) aCGGCGTAGGTTCTGACGTTTATGGCGTGACCTCCTTCACCGAGCTGGCGCGGGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCg < 2:758752/143‑1 (MQ=255) cGGCGTAGGTTCTGACGGTTAGAGCGTGACCTCCGTCACCGAGCTGGCGCGCGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGCAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCgg < 2:56733/143‑1 (MQ=255) ggCGTAGGTTCTGACGTTTATAGCGTGACATCCTGCACCGAGCTAGCGCGTGATGGTCAGGATTGTGAAAGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGGGTATCGCTTGGGTGATGAACGGCGCCCCCGc > 1:719349/1‑143 (MQ=255) gACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc > 1:645953/1‑143 (MQ=255) ataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtct > 1:164824/1‑141 (MQ=255) agaGCGTGCCCTCCCGCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAATCTCCTCTCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtat < 1:701076/141‑1 (MQ=255) ctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGt > 1:263677/1‑143 (MQ=255) ctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCCCCGGCAGTGGCGCCTACCGACTATATGAAACTGt > 2:112553/1‑143 (MQ=255) ctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCCACGACGGTGGCATATACCGACTATATGAAACTga > 2:46501/1‑142 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGGATATCGCTTAGGTGATGAACGACACTCCGGCAGTGGCATCTACCCACTATATGAAACTGtt > 1:698485/1‑143 (MQ=255) cctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:79296/143‑1 (MQ=255) cctCCAGCGAGCGGACGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGAGGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:752196/143‑1 (MQ=255) cGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc < 1:203534/143‑1 (MQ=255) cGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGGGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGc > 2:739569/1‑143 (MQ=255) tgCTGGCGCGTGATGGTCAGGATTGGGGGCGCTGGAACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAg < 2:152151/142‑1 (MQ=255) cTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCAGGCAGTAGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt > 2:566730/1‑143 (MQ=255) cgcgcGTGATGGCCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 1:540551/142‑1 (MQ=255) tGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGCGCAGGTCCGTACtt > 2:180478/1‑143 (MQ=255) tGATGGGCAGCAGTGTGAACGCTGGAACAGGCCGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACtt < 1:625572/143‑1 (MQ=255) tGACGGTCCGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACtt < 1:56794/143‑1 (MQ=255) cAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtga > 1:248647/1‑70 (MQ=255) cAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtga < 2:248647/70‑1 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCAGGAAACTCCGCGCGTTCCGTATAACGATAAGCAGATGAGCGCCGCTCCGGCAGTGGCATCTACCGTCTATATAAAACTGTTCGCTGAGCAGGCCCGTACTAACGTAGCGGc > 2:514621/1‑143 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTCCTTACGGACCGGCTgacgcc > 1:466044/1‑141 (MQ=255) gCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAc < 1:499812/143‑1 (MQ=255) ctgcCCCCGCTGGAAACGCCGCGCGTGCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTggg < 1:482966/143‑1 (MQ=255) tgcACCCGCTGGAAACTCCGCGCGTTCCGTATATAGCGTAGGTGATGAACGACGCTCCGGCAGTGGCAACTACCGACTATCTGAGACTGTACGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGCCCACCGCGTACTAGGt > 2:272306/1‑143 (MQ=255) ggCTGGAAACGCCGCGCGTGCCGTGTATCGCTTAGGTGATGAACGACGGTCCGGCAGTGGCATCTACCGACTAGATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 2:9080/142‑1 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACCGTTCGCTGAACAGGTCCGTACTTACGTACCGGATGACGGCTACCGGGTACTGGGTACTGAt > 2:218500/1‑143 (MQ=255) gAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGCACTGATGGCtt > 2:709378/1‑143 (MQ=255) cgcgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTCCGTACCGGCTGCCGACTACCGCGTACTGGGCACCGAGGGCTTCGGTCGt > 2:331545/1‑143 (MQ=255) ttCCGTATATCGCTTAGGTGATGAACGACGCTCCGACAGTGGCAGCGGCCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTGGCGCGTGCTGGGGACTGATGGCTGCGGTGGGTCCGAc > 2:55417/1‑143 (MQ=255) ccGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAg < 1:431696/143‑1 (MQ=255) ccGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACCTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAg < 1:657808/143‑1 (MQ=255) cGTATACCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGc < 1:509470/143‑1 (MQ=255) tCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGCCAGCCGTgag > 1:232310/1‑143 (MQ=255) cGCTTAGGGGATGAACGACGCTCCGGCAGTGGCGTCTACCGATTATATGAAACTGTGCGCTGGGCCGGTCCGTACTTACGTACAGGCTGACGACTACCGCGTACTGGGTGCTGATGGCTTCGGGCGTTCCGACAGCCgaggga > 2:417118/1‑138 (MQ=255) | AAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA > NC_000913/125258‑125525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |