Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	86.6 / NA	29	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/14);  total (15/14)

TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132655‑2132926                                                                                                                                     |                                                                                                                                            gagagGGTAGATAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   <  2:499980/142‑1 (MQ=255)            cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGCATTGTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                        <  2:195100/143‑1 (MQ=255)               cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTcc                                                                                                                     >  1:462228/1‑143 (MQ=255)                tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  1:636207/143‑1 (MQ=255)                cgctatTTCTTGCTGGCGCGGCCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  2:454491/140‑1 (MQ=255)                       ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg                                                                                                             >  1:780856/1‑143 (MQ=255)                                     tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac                                                                                               >  2:697103/1‑143 (MQ=255)                                          tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCAGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTATCCACGGTTCGCCGTACTCCACCACCATcc                                                                                          >  1:504315/1‑143 (MQ=255)                                           ttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACGCCCATCCg                                                                                         >  1:189352/1‑143 (MQ=255)                                                     gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTCACGGTGACGATGGGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                               <  1:649846/143‑1 (MQ=255)                                                        tgctctAAATGCGGATGTGCCCGGTGAATTTTTTCGTGGGCGTGCTGATCTGCTTTTCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                            <  2:133385/138‑1 (MQ=255)                                                        tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                            <  1:781412/143‑1 (MQ=255)                                                            gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGCGTCGCCGTACTCCCCCCCCATCCGCCGGGAGATGCCGCAAt                                                                        >  1:66102/1‑143 (MQ=255)                                                                  gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATAGAGACGTATATGTCGTAATCGtgtg                                                                  >  2:714870/1‑143 (MQ=255)                                                                    ggAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAGGCTGTCGTAATCCTGTGcc                                                                >  2:258491/1‑143 (MQ=255)                                                                         tccccGGAGAACTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           <  1:376345/141‑1 (MQ=255)                                                                           cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:588160/143‑1 (MQ=255)                                                                              gcagagTTTTTTCGCTGACCAGGTGATCTGCATTTCCCCGTGATTGATATGCACGCTCTGCGTCGCCTCTCCCCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  1:60198/141‑1 (MQ=255)                                                                                          cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                          <  2:576250/143‑1 (MQ=255)                                                                                            gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGCCGTAATCCTGTGCCGCTCTCAGCTCGGCACCGaacaga                                        >  2:691677/1‑141 (MQ=255)                                                                                                 gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTCAGGTATCGAACAAacaccc                                   >  1:727601/1‑141 (MQ=255)                                                                                                  tggggATCTGCTTTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACGTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                                  <  2:446317/139‑1 (MQ=255)                                                                                                  gtgtgtTCTGCATTCCACCGTGGTTGATATGCGGGCCCGGCGGCTCCTCTGCTACTTTCCACGGTGCGCCGTTCTCCTCGTCCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                                  <  2:736202/141‑1 (MQ=255)                                                                                                    gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAGCCTCCGCAGGAAGATGTCGTAATCCTGTCCCGCTGTCCGTTGGGTATCCaccaaacagcattt                                >  2:108808/1‑137 (MQ=255)                                                                                                      gctCTGCATTTCACCGTGCTCGATGTGCCGGCTCTGCGTCGCCTCTTCGACTTTCCCTGGTTCGCCGTCCGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                              <  2:486673/141‑1 (MQ=255)                                                                                                            cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCATGAAGATGTCGTAATACTGTGCCGCTTTCAATTCGGTGTAGAACAAACACACTTGGAAAcaca                        >  2:422621/1‑140 (MQ=255)                                                                                                                             tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGCAGATGTCTTAATCGTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCCTGGCCCGGTAACGAcc       >  1:533945/1‑143 (MQ=255)                                                                                                                                  aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCACGCCCAGGTACAGACCCGGtc  >  2:759118/1‑142 (MQ=255)                                                                                                                                  aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCCGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTCAAGACCTgggt  >  1:759338/1‑141 (MQ=255)                                                                                                                                     |                                                                                                                                            TAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132655‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑