Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,969	C→G	V55L (GTT→CTT)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,969	0	C	G	100.0%	133.6 / NA	43	V55L (GTT→CTT)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/26);  total (17/26)

CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661831‑2662108                                                                                                                                           |                                                                                                                                            cgccCTGGCCGCCGCCTTTCCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACCGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa                                                                                                                                         <  2:327184/143‑1 (MQ=255)                 tttACCCTGTCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTCCGCAGAc                                                                                                                         <  2:597438/143‑1 (MQ=255)                      ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGACAGCAGACCCTTTTTACGCAGACgggca                                                                                                                    >  1:709574/1‑141 (MQ=255)                                   ggCATCTACAGACTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa                                                                                                       <  1:413628/143‑1 (MQ=255)                                     cATCTACAGACTCGTCAACTGCGCTAAGTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa                                                                                                     <  1:345658/143‑1 (MQ=255)                                      aTCTACAGATTCGTCAACGGCGCTAGTTGCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt                                                                                                    <  1:165460/143‑1 (MQ=255)                                       tCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATa                                                                                                   <  1:475842/143‑1 (MQ=255)                                          aCAGATTCGACAACGGCGCTAACTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCATGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa                                                                                                <  1:437995/143‑1 (MQ=255)                                            aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                        <  2:43161/133‑1 (MQ=255)                                            aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                        >  1:43161/1‑133 (MQ=255)                                            aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                              <  1:647194/143‑1 (MQ=255)                                            aGATTCGTCAACGGCGCTAATTACGTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                              <  1:52077/143‑1 (MQ=255)                                              cttCGTCAACGGCGCTAATTACTTCCCCAACGGCGACGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                            <  1:579795/142‑1 (MQ=255)                                              aTTCGTCACCGGCGCTAATTACTTCGCCAACCGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg                                                                                            <  2:781849/143‑1 (MQ=255)                                                   tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                       >  1:732342/1‑143 (MQ=255)                                                        ggCGCTAATTACGTCGCCAACGGCGATGCTGCTGGCATCGTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                  <  2:407085/143‑1 (MQ=255)                                                              aaTTACGTCGGCAGCGGCGAGGCTGCTGGCATCTGTGCCTAACAGATAACCTTCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGtt                                                                            <  2:120128/143‑1 (MQ=255)                                                                 tACGTCTCCAACGGCGATGCTGCTTGCATCTTGGCCTAACAAATTTCCTTCTCCTGGTCCACGTACGCTGGATAGCAGACCATTTTTACGCAGACTGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg                                                                         <  2:674243/143‑1 (MQ=255)                                                                    ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                      <  1:67435/143‑1 (MQ=255)                                                                           aCGGCGATGCTGCTGACATCGTTGCCTAACAGAGAACCACCGCCTGGTGCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGCTGACGCAGGGAAATCCCCTGACGTTCGGAAATATCAGc                                                               <  2:640923/143‑1 (MQ=255)                                                                           aCGGCGAGGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc                                                               <  1:204017/143‑1 (MQ=255)                                                                             ggCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTGTACGCAGACGGGAAAACAGTTGCTCCAGATAAGAAAGGGAAATTCCCTGACGATCGGAAATATCAGCCa                                                             >  2:128501/1‑143 (MQ=255)                                                                                     tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAcc                                                     >  1:146322/1‑143 (MQ=255)                                                                                       ctgGCATCTTTGCCTAACCGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGAAGGGAAAACATTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCgg                                                   <  1:200456/143‑1 (MQ=255)                                                                                                ttGCCTAACGGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTc                                          <  2:413323/143‑1 (MQ=255)                                                                                                  gCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag                                        <  1:661863/143‑1 (MQ=255)                                                                                                   ccTAACAGATAACCACCGCCGGGTCCACGTACGCTGGAAAGCAGACCATTTTTACCCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                       <  1:90672/143‑1 (MQ=255)                                                                                                     tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCATCGGTACCGGGCCCGCTTCAGAGt                                     >  1:332210/1‑143 (MQ=255)                                                                                                         aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAAGAGGTGGTCCAGATAAGGAAGGCAAATTCCCTGACGTTCGGAAATATCAGCGAACGGTACCGGGCCCGCTTCAGAGtggcg                                 >  2:201478/1‑139 (MQ=255)                                                                                                          gATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                                <  2:144262/143‑1 (MQ=255)                                                                                                             aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATAACAGCCAACGGTACCGGGGCCCCTGCAGAGTTGAgcgga                             >  2:304984/1‑141 (MQ=255)                                                                                                                caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCCGAGTGGAGAGCCACg                          >  2:493536/1‑143 (MQ=255)                                                                                                                caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCACGCTGCAGAGTTGAGCGCAACg                          >  1:153776/1‑143 (MQ=255)                                                                                                                caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAAGGAAATTCCCTGACGTTAGGAAATCTCACCCAACAATACCAGGCCCGCATCAAAGTTGAGAGTAAca                          >  2:342669/1‑142 (MQ=255)                                                                                                                 accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAGAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGGCCGCTTCAGAGTTGAGCGTAACGt                         >  2:180372/1‑143 (MQ=255)                                                                                                                          tCCACGTACGCTGGAAAGCAGCTCATTGTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCCACGGTACCGGGCCCGCTTCAGAGTTGAGCCCAACGTCAAGCATTg                <  2:658856/143‑1 (MQ=255)                                                                                                                             gcGTACGCCGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCgg             <  2:620350/142‑1 (MQ=255)                                                                                                                             aCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCgg             >  1:614343/1‑143 (MQ=255)                                                                                                                             aCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCTCTTCAGAGTTGAGCGCAACGTCAAGCATTGCgg             >  1:402367/1‑143 (MQ=255)                                                                                                                                 aCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGCAAAGAAAGAGAAATTCCCTCCCGTTCGGAAATATCGGCCAACGGTACCGGGCCGGCTTCAGAGTTGAGCGCCACGTCACGCATTGCAGTCAc         >  1:179974/1‑143 (MQ=255)                                                                                                                                   gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg       >  1:290367/1‑143 (MQ=255)                                                                                                                                       gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa   >  1:761138/1‑143 (MQ=255)                                                                                                                                        aaaGCAGACCCTTTTTTCGCATGCTCGAAAACTGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAg  <  2:665286/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661831‑2662108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑