Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	97.7%	127.7 / NA	45	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (22/21);  minor base C (0/1);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041492‑3041767                                                                                                                                             |                                                                                                                                        cgccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                       >  1:544100/1‑143 (MQ=255)        tGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgc                                                                                                                                <  1:367867/143‑1 (MQ=255)          cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACCATCgcgc                                                                                                                              >  2:15485/1‑143 (MQ=255)          cGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                              >  1:128096/1‑143 (MQ=255)           gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCACATCGCGcc                                                                                                                             >  2:460984/1‑143 (MQ=255)           gACGCCAAAGGTAAAATGTGGAGCAATGTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                             <  1:135492/143‑1 (MQ=255)                cAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg                                                                                                                        >  1:544990/1‑143 (MQ=255)                 aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga                                                                                                                       >  1:244361/1‑143 (MQ=255)                                aGCAATTTACGTCTGTTCCGCGACGGCGATGGCATTGCATGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctgcc                                                                                                        >  2:168676/1‑142 (MQ=255)                                 gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCtt                                                                                                       <  2:438935/143‑1 (MQ=255)                                    ctttACGTCTTTTCCGCTACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGCACCGGAGCTGTCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                    <  1:777429/142‑1 (MQ=255)                                    aggtACGGCGGTGCCGAGAGGGTGAGGGCGTGGCATGGATGTAACGGCGTAGCGGGGGTGAACCGCAGCTGAATGAACTGAATGAATATGCGGTATTCTCTAAAGCGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                    <  2:468325/140‑1 (MQ=255)                                       gaCGTCGGTGCCGCGACGGGGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGtt                                                                                                 <  2:573736/142‑1 (MQ=255)                                           tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                             >  2:438459/1‑143 (MQ=255)                                                             tGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                                                           <  1:721044/143‑1 (MQ=255)                                                                cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGcgcgccgcgc                                                                        >  2:339759/1‑143 (MQ=255)                                                                   ggCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                     <  1:726359/142‑1 (MQ=255)                                                                     cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTCAACAGACCATCGCTCCAGACCACCAGCGCCTCCTGCATAGTGCTGCCACTTTTCAGACGCGCCCCGCGCTAGCa                                                                   >  1:593170/1‑143 (MQ=255)                                                                      aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                  >  1:44943/1‑143 (MQ=255)                                                                      aTGGATTGAACGGCGCAGCATGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCTAACGACGAGCGTGTGCTGCTTGGTGTTGCCGGACTTCAGGCGCCCGGCGCGCCGGCac                                                                  >  2:690787/1‑142 (MQ=255)                                                                          aTTGGACGGCGCAGCGTGCGTGAGCCGCAGCTGACTGAACTGAAAAACTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGGCGAGCGTGTGCTGCCTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                              <  2:382677/143‑1 (MQ=255)                                                                           ttGAGCGGCGCAGCGGGCGTGAACCGCGGCTGACTGAACTGAAAATATATGCGGTATTCTCGAAGGGGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                             <  2:724002/143‑1 (MQ=255)                                                                             gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                                                           <  1:575419/143‑1 (MQ=255)                                                                                  gcgcAGCGTGCGTGAACCGCAGCTGACTGAACTGGAAAGATATGCGGTCTTCTATTAAGAGGTCATCGAGTCGGACGACGAGCGTGTGCTGCTTGGTGTTGCAGGTTTTCATGCGAGGGCCGCGCTGGCAAATCTCTTTAGCg                                                      <  2:14849/143‑1 (MQ=255)                                                                                  gcgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGAGCAAGACGACGAGCGTGTGCTGCTAGGTGTAGCCGGTGTTCAGGCGAGCGCCGCGATGGCAACACGCTTTACCg                                                      >  2:206731/1‑143 (MQ=255)                                                                                   cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa                                                     >  1:114660/1‑143 (MQ=255)                                                                                    gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCGGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCGTTAGCGaa                                                    >  1:217126/1‑143 (MQ=255)                                                                                      aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACt                                                  <  2:321506/143‑1 (MQ=255)                                                                                              tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                    >  2:56752/1‑85 (MQ=255)                                                                                              tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                    <  1:56752/85‑1 (MQ=255)                                                                                                  ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGGGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCCTCgaaagg                                      >  1:374723/1‑142 (MQ=255)                                                                                                            ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            >  2:302138/1‑143 (MQ=255)                                                                                                            ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGACAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  2:34718/143‑1 (MQ=255)                                                                                                              caactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGt                          <  1:15485/142‑1 (MQ=255)                                                                                                                 ctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaa                       <  1:431983/143‑1 (MQ=255)                                                                                                                  tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa                      >  1:679429/1‑143 (MQ=255)                                                                                                                  tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCGGTAGCGAACTGCCTGCGAAAGAAAAACAGGCAGTCaaa                      >  1:1173/1‑143 (MQ=255)                                                                                                                  tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGATTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAGGAAAAACCGGTAGGCaaa                      >  2:499176/1‑143 (MQ=255)                                                                                                                   gaaAAAATATGCGGGATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag                     <  2:572632/143‑1 (MQ=255)                                                                                                                     aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                   <  2:544100/143‑1 (MQ=255)                                                                                                                      aaaaTATGCGGTATTCGCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTATTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTTAGCGAACTGCCTACGAAAGAAAAACAGGTAGTCAaagaag                  >  2:246390/1‑143 (MQ=255)                                                                                                                          tatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc              <  2:544990/143‑1 (MQ=255)                                                                                                                           atGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGcgcg             <  1:433135/143‑1 (MQ=255)                                                                                                                                   ttCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt     <  1:550396/143‑1 (MQ=255)                                                                                                                                     ctctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc   <  1:302138/143‑1 (MQ=255)                                                                                                                                      tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTATGCt  >  2:779930/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                        CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041492‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑