Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,231	C→A	E26* (GAA→TAA)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,231	0	C	A	100.0%	149.0 / NA	47	E26* (GAA→TAA)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/28);  total (19/28)

GCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCC  >  NC_000913/4640097‑4640356                                                                                                                                       |                                                                                                                              gcgcAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCAGAGTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCGTCGAAAACATCATAGCCTTACGCTTCGa                                                                                                                       <  2:180753/143‑1 (MQ=255)      gTTCACGCGCTAAGATAAGACCGTTCTTACCCGGCAGGCTGAGATCCACGATCACCAGGTGGCGGTCACGATCAGAGAGGACCTAATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATa                                                                                                                  <  2:636830/143‑1 (MQ=255)         cACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACAAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCAGCTGTCGCATCGAAAAGATCATAGCATTACGCGCCGACAATACtc                                                                                                               >  2:574816/1‑142 (MQ=255)          aCGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACttt                                                                                                              <  1:575891/143‑1 (MQ=255)               cTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAAc                                                                                                         <  1:274469/143‑1 (MQ=255)                 aaCAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgt                                                                                                       <  2:33863/143‑1 (MQ=255)                        gaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCgt                                                                                                <  1:685678/143‑1 (MQ=255)                        gaCCGTTCTCACCCGGCAGATTGATATCCATGCTCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCgt                                                                                                <  2:739806/143‑1 (MQ=255)                               cTTACCCGACAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCa                                                                                         >  1:134818/1‑143 (MQ=255)                                   cccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:514525/143‑1 (MQ=255)                                   cccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:781396/143‑1 (MQ=255)                                   cccGGCAGATTGAGATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:56275/143‑1 (MQ=255)                                    ccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGCAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg                                                                                    >  2:215075/1‑143 (MQ=255)                                    ccGGCAGATTGATATCCATGATCAACAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCTGCGCCCTCTGTCGCTTCGCCAACATCCTAGCCTTACGCTTAGAAAATAATTTTCAACGTGTTAAGTGTTACAAACTCg                                                                                    >  1:30870/1‑143 (MQ=255)                                         aGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCACCTAGGCTTc                                                                               >  2:663716/1‑143 (MQ=255)                                            tagATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc                                                                            <  2:332443/141‑1 (MQ=255)                                              gATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa                                                                          >  2:10232/1‑143 (MQ=255)                                                  tCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAg                                                                      >  1:715120/1‑143 (MQ=255)                                                           aCCAGGTTGATGTCATATTCAGAGAGGATCCGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCGTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGCCTTCAACGATAAGAATGTGCgg                                                             <  2:350039/143‑1 (MQ=255)                                                              aGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCCTCTGTCGCTTCGCAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTCGCGTGCTACCAACACGTCTTCCACGATAAGCATGCGCGGAGt                                                          >  2:694177/1‑143 (MQ=255)                                                                 ttGATGTCATAGTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGACAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTGGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg                                                       <  2:529728/143‑1 (MQ=255)                                                                      gTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtgt                                                                                                     >  2:569563/1‑92 (MQ=255)                                                                      gTCATAAGCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtgt                                                                                                     <  1:569563/92‑1 (MQ=255)                                                                        cagatTCAGGGAGTGTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGGTAAGAATGTGCGGGGTCTGCATGttt                                                <  1:678600/140‑1 (MQ=255)                                                                           atTAAGAGAAGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCt                                             <  1:380961/143‑1 (MQ=255)                                                                                gagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCCACGTGTTGCGTGTTACCAACTCGTCTTCCACGATAGGGATGTGCGGGGTGTGCAGGATTGCTAccaa                                        >  2:488422/1‑141 (MQ=255)                                                                                gagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa                                        <  1:541148/143‑1 (MQ=255)                                                                                   agGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                     >  1:191391/1‑143 (MQ=255)                                                                                      aTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGc                                  <  2:134818/143‑1 (MQ=255)                                                                                       tctgtgccATTCCCTCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGcc                                 <  2:184969/136‑1 (MQ=255)                                                                                       tCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCCACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATCTTTGCTACCTaaacggcc                                 >  1:36714/1‑138 (MQ=255)                                                                                       gcTGGTGCATTTCCGCGTCGTCTGTCGCTTCGAAGACATCATAGCCTTACGCTTCGAAAATACTTGTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGcc                                 <  2:667152/142‑1 (MQ=255)                                                                                         tGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCaa                               <  1:368602/143‑1 (MQ=255)                                                                                          gATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACGTAAATTGGCAAc                              >  1:437581/1‑143 (MQ=255)                                                                                              cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaaa                          <  1:258992/143‑1 (MQ=255)                                                                                              cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaaa                          <  2:59525/143‑1 (MQ=255)                                                                                                    cgcgCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCCATGGCAAGAAGGGGTGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                    >  1:775191/1‑143 (MQ=255)                                                                                                     gcgcCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCCACTACATCgtgcc                   >  1:69715/1‑139 (MQ=255)                                                                                                       gcCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAg                 >  1:627069/1‑143 (MQ=255)                                                                                                          gtCTGTCTCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGaa              <  2:488530/142‑1 (MQ=255)                                                                                                           tCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAg             <  1:371344/143‑1 (MQ=255)                                                                                                             tctCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCGAAATTGCCAACTAAATCGAAACAGGAAGTa           <  1:279219/141‑1 (MQ=255)                                                                                                             tGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTa           >  1:716812/1‑143 (MQ=255)                                                                                                               gcgcgTCGAAAACATCATAGCCTTACGCTGCGAAGATACTTTTCAACGTGGTGCGGATCACCACCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTGTGCTACCTAAATGGCCAACTAAATCGAAACAGGAAGTACa         <  2:586283/142‑1 (MQ=255)                                                                                                                  cTTCGAAAACATCATAGCCTTACGCTGCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaaaa      <  2:179572/143‑1 (MQ=255)                                                                                                                      gAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGGATGTGCGGGGTCTGCATGTTTGCTACCTACATTGCCCACTCAATCGAAACAGGAAGTAAAAAAGTca  >  1:475652/1‑142 (MQ=255)                                                                                                                      gAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAAGATAAGAATGTGCGGGGACTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTAGAAAAGAcc  >  2:190946/1‑143 (MQ=255)                                                                                                                                       |                                                                                                                              GCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCC  >  NC_000913/4640097‑4640356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑